公开(公告)号：US20240299513A1

公开(公告)日：2024-09-12

申请号：US18572241

申请日：2022-06-22

申请人： The Trustees of Columbia University in the City of New York

发明人： Remi J. Creusot ,  Rebuma Firdessa Fite ,  Jorge Postigo Fernandez

IPC分类号： A61K39/00 ,  A61P3/10

CPC分类号： A61K39/0008 ,  A61P3/10 ,  A61K2039/53 ,  A61K2039/55555

摘要： This disclosure describes a nucleic acid construct that contains sequences for an Endotope construct, a STAT1c, and miR142 target sites. In one example, disclosed is composition comprising an Endotope construct and a STAT1 construct including a nucleic acid sequence encoding a constitutively active STAT1 (e.g. STAT1c), wherein the Endotope and the STAT1 constructs each include miR142 target sites. In alternative examples, disclosed is a single construct that includes the Endotope construct and STAT1 construct along with miR142 target sites. The nucleic acid constructs can be packaged into polycationic molecules or liposome to create nanoparticles for efficient cell transfection.