公开(公告)号：US20110118132A1

公开(公告)日：2011-05-19

申请号：US13012831

申请日：2011-01-25

申请人： Theodore Winger ,  Allen E. Eckhardt ,  Carrie Graham ,  Ramakrishna Sista ,  Vamsee K. Pamula ,  Tong Wang ,  Yalin Xiong ,  Ning Wu ,  Sanjay Saha ,  Gajendrasinh Balvantsinh Raolji ,  Raveendra Dayam

发明人： Theodore Winger ,  Allen E. Eckhardt ,  Carrie Graham ,  Ramakrishna Sista ,  Vamsee K. Pamula ,  Tong Wang ,  Yalin Xiong ,  Ning Wu ,  Sanjay Saha ,  Gajendrasinh Balvantsinh Raolji ,  Raveendra Dayam

IPC分类号： C40B30/00 ,  C12Q1/00

CPC分类号： C12Q1/00

摘要： The invention is directed to droplet actuator devices and assay methods. The method may include, among other things, a method of conducting enzymatic assays using umbelliferone substrates with cyclodextrins in droplets in oil, the method including incubating a droplet in oil, the droplet preferably comprising an umbelliferone substrate, a sample, and a cyclodextrin compound. The methods may further include a method of substantially eliminating cross-contamination between droplets in enzymatic containing substrate-based bioassays. The method may include immobilization of the enzymatic substrate including forming an inclusion complex with the substrate for stabilizing the substrate within an aqueous environment, wherein the inclusion complex may be formed using cyclodextrins. In yet another embodiment, the invention may provide a method of enhancing hydrolysis of enzymatic substrates. The methods may further include a method of forming an inclusion complex with the substrate for stabilizing the substrate within an aqueous environment, wherein the substrate may be a 4-MU- or HMU containing substrate. The inclusion complex may be formed using cyclodextrin.

摘要翻译： 本发明涉及液滴致动器装置和测定方法。 该方法尤其可以包括使用具有环糊精的伞形酮底物在油中的液滴进行酶测定的方法，所述方法包括将液滴在油中孵育，所述液滴优选包含伞形酮底物，样品和环糊精化合物。 所述方法还可以包括基本上消除含酶底物生物测定中的液滴之间的交叉污染的方法。 该方法可以包括固定酶底物，包括与底物形成包合物以使其在水性环境中稳定，其中包合物可以使用环糊精形成。 在另一个实施方案中，本发明可提供增强酶底物水解的方法。 所述方法还可以包括在水性环境中与基材形成包含配合物以稳定基材的方法，其中所述基材可以是含有4-MU-或HMU的底物。 包合物可以使用环糊精形成。