公开(公告)号：US20160215271A1

公开(公告)日：2016-07-28

申请号：US14904315

申请日：2014-05-21

Applicant: Timo VESIKARI ,  Vesna BLAZEVIC ,  UMN PHARMA INC.

Inventor： Masahiro OKADA ,  Akiko MUKAI ,  Tomonori NISHINO ,  Daisuke ARINOBU ,  Hiroyuki TO ,  Mamoru SATOH

IPC: C12N7/00

CPC classification number: C12N7/00 ,  A61K39/12 ,  A61K2039/5256 ,  A61K2039/5258 ,  C07K14/005 ,  C12N2710/14043 ,  C12N2770/16022 ,  C12N2770/16023 ,  C12N2770/16034 ,  C12N2770/16051

Abstract: The present invention aims at establishing a means for obtaining highly pure virus-like particles by a simple method. There is provided a production method for culture containing virus-like particles, comprising: transforming insect cells with baculovirus vector containing a viral nucleic acid sequence; and cultivating the insect cells, wherein the culture is obtained after continuing cultivating the insect cells until the viability of the insect cells reaches 10% or less.

Abstract translation: 本发明旨在通过简单的方法建立获得高纯度病毒样颗粒的方法。 提供含有病毒样颗粒的培养物的制备方法，包括：用含有病毒核酸序列的杆状病毒载体转化昆虫细胞; 培养昆虫细胞，其中在继续培养昆虫细胞直到昆虫细胞的存活率达到10％以下之前获得培养物。

公开(公告)号：US20160168543A1

公开(公告)日：2016-06-16

申请号：US14904364

申请日：2014-05-21

Applicant: Timo VESIKARI ,  Vesna BLAZEVIC ,  UMN PHARMA INC.

Inventor： Masahiro OKADA ,  Akiko MUKAI ,  Tomonori NISHINO ,  Daisuke ARINOBU ,  Hiroyuki TO ,  Mamoru SATOH

IPC: C12N7/00

CPC classification number: C12N7/00 ,  C12N2770/16023 ,  C12N2770/16051

Abstract: The present invention aims at establishing a novel means for purifying norovirus VLPs. There is provided a purification method of norovirus virus-like particles, comprising: contacting solution containing norovirus virus-like particles with support for hydroxyapatite chromatography, binding the virus-like particles to the support, subsequently washing the support with buffer, and then eluting the virus-like particles from the support with buffer containing phosphate, wherein the phosphate concentration of the buffer used for elution is less than 10 mM.

Abstract translation: 本发明旨在建立一种纯化诺如病毒VLP的新方法。 提供了诺如病毒病毒样颗粒的纯化方法，其包括：将含有诺如病毒病毒样颗粒的溶液与羟基磷灰石层析用载体接触，将病毒样颗粒结合到载体上，随后用缓冲液洗涤载体， 来自含有磷酸盐缓冲液的载体的病毒样颗粒，其中用于洗脱的缓冲液的磷酸盐浓度小于10mM。