公开(公告)号：US06274384B1

公开(公告)日：2001-08-14

申请号：US09346039

申请日：1999-07-06

申请人： Timothy W. Starzl ,  David W. Nunnery ,  MaryBeth Robinson ,  H. John Hanlin

发明人： Timothy W. Starzl ,  David W. Nunnery ,  MaryBeth Robinson ,  H. John Hanlin

IPC分类号： G01N33543

CPC分类号： G01N33/525 ,  Y10S435/808 ,  Y10S436/805 ,  Y10T436/113332 ,  Y10T436/116664

摘要： A system for detecting a specific substance or analyte of interest is provided that includes one or more sensing units and an instrument for analyzing the sensing units. Each sensing unit preferably includes a substrate, an attachment layer and at least one capture layer that includes a ligand layer. In one embodiment, the attachment layer is tripartite and includes a lower binding surface held to the substrate and an upper binding surface that holds the ligand layer, together with an insulating layer disposed between these two surfaces. The lower binding surface provides a durable and stable attachment to the substrate. The upper binding surface holds the ligand layer and does not jeopardize the integrity or viability thereof. The insulating layer prevents any unwanted interaction between the lower and upper binding surfaces. Each sensing unit is supported on a test piece received by the instrument. The instrument controllably positions the test piece using marks and/or codes on the test piece. The instrument measures a difference in mass in the sensing unit in connection with determining whether or not the analyte of interest is present. A light beam is used in making this measurement. In one embodiment, multiple reflections of the light beam on the same sensing unit are utilized to improve the sensitivity of the instrument. Mass enhancement techniques are also preferably utilized to effectively amplify the detected signal that is indicative of the analyte's presence. The system can also include a device for heating, humidifying and mixing materials of the sensing units, as well as preventing cross-contamination of the sensing units.

摘要翻译： 提供了用于检测感兴趣的特定物质或分析物的系统，其包括一个或多个感测单元和用于分析感测单元的仪器。 每个感测单元优选地包括基底，附着层和包括配体层的至少一个俘获层。 在一个实施例中，附着层是三方的并且包括保持在基底上的下部结合表面和保持配体层的上部结合表面以及设置在这两个表面之间的绝缘层。 下部结合表面提供了对基底的持久和稳定的附着。 上部结合表面保持配体层，并且不危及其完整性或可行性。 绝缘层防止下部和上部结合表面之间的任何不需要的相互作用。 每个感测单元都支持在由仪器接收的测试片上。 仪器可以使用标记和/或代码可靠地将测试片定位在测试片上。 在确定感兴趣的分析物是否存在的情况下，仪器测量感测单元中的质量差异。 使用光束进行此测量。 在一个实施例中，利用相同感测单元上的光束的多次反射来提高仪器的灵敏度。 还优选使用质量增强技术来有效地放大指示分析物存在的检测信号。 该系统还可以包括用于加热，加湿和混合感测单元的材料的装置，以及防止感测单元的交叉污染。

公开(公告)号：US5958704A

公开(公告)日：1999-09-28

申请号：US820365

申请日：1997-03-12

申请人： Timothy W. Starzl ,  David W. Nunnery ,  MaryBeth Robinson ,  H. John Hanlin

发明人： Timothy W. Starzl ,  David W. Nunnery ,  MaryBeth Robinson ,  H. John Hanlin

IPC分类号： G01N21/64 ,  G01N33/52 ,  G01N33/543 ,  G01N33/53

CPC分类号： G01N33/525 ,  Y10S435/808 ,  Y10S436/805 ,  Y10T436/113332 ,  Y10T436/116664

摘要： A system for detecting a specific substance or analyte of interest is provided that includes one or more sensing units and an instrument for analyzing the sensing units. Each sensing unit preferably includes a substrate, an attachment layer and at least one capture layer that includes a ligand layer. In one embodiment, the attachment layer is tripartite and includes a lower binding surface held to the substrate and an upper binding surface that holds the ligand layer, together with an insulating layer disposed between these two surfaces. The lower binding surface provides a durable and stable attachment to the substrate. The upper binding surface holds the ligand layer and does not jeopardize the integrity or viability thereof. The insulating layer prevents any unwanted interaction between the lower and upper binding surfaces. Each sensing unit is supported on a test piece received by the instrument. The instrument controllably positions the test piece using marks and/or codes on the test piece. The instrument measures a difference in mass in the sensing unit in connection with determining whether or not the analyte of interest is present. A light beam is used in making this measurement. In one embodiment, multiple reflections of the light beam on the same sensing unit are utilized to improve the sensitivity of the instrument. Mass enhancement techniques are also preferably utilized to effectively amplify the detected signal that is indicative of the analyte's presence. The system can also include a device for heating, humidifying and mixing materials of the sensing units, as well as preventing cross-contamination of the sensing units.

摘要翻译： 提供了用于检测感兴趣的特定物质或分析物的系统，其包括一个或多个感测单元和用于分析感测单元的仪器。 每个感测单元优选地包括基底，附着层和包括配体层的至少一个俘获层。 在一个实施例中，附着层是三方的并且包括保持在基底上的下部结合表面和保持配体层的上部结合表面以及设置在这两个表面之间的绝缘层。 下部结合表面提供了对基底的持久和稳定的附着。 上部结合表面保持配体层，并且不危及其完整性或可行性。 绝缘层防止下部和上部结合表面之间的任何不需要的相互作用。 每个感测单元都支持在由仪器接收的测试片上。 仪器可以使用标记和/或代码可靠地将测试片定位在测试片上。 在确定感兴趣的分析物是否存在的情况下，仪器测量感测单元中的质量差异。 使用光束进行此测量。 在一个实施例中，利用相同感测单元上的光束的多次反射来提高仪器的灵敏度。 还优选使用质量增强技术来有效地放大指示分析物存在的检测信号。 该系统还可以包括用于加热，加湿和混合感测单元的材料的装置，以及防止感测单元的交叉污染。

公开(公告)号：US20090069694A1

公开(公告)日：2009-03-12

申请号：US12286850

申请日：2008-10-02

申请人： David Amundson ,  H. John Hanlin ,  Larry Blankenship

发明人： David Amundson ,  H. John Hanlin ,  Larry Blankenship

IPC分类号： A61B8/14 ,  A61B1/04

CPC分类号： A61B5/0084 ,  A61B5/0086 ,  A61B8/12

摘要： A coronary access catheter system simplifies the insertion of objects into distal branches of the coronary sinus. The system incorporates a real-time forward-imaging means to view the os and the branches of the coronary sinus. Preferably, the catheter uses near-infrared light as the forward-imaging means, but it could also include ultrasound or electromagnetic transducer. As the image is viewed, the catheter tip can be steered into the coronary sinus os and deflected in a tight radius bend on the distal end to navigate the short radius, right angle turns found in the coronary sinus branches. At that point, a flexible sheath can be placed over the guide catheter or objects such as guidewires can be inserted into channels of the guide catheter. The system consists of a catheter and image acquisition unit, which displays the forward image.

摘要翻译： 冠状动脉导管系统简化了将物体插入冠状窦的远端分支。 该系统包含实时前进成像装置以观察冠状窦的手术和分支。 优选地，导管使用近红外光作为正向成像装置，但是也可以包括超声波或电磁换能器。 当观察图像时，导管尖端可被引导到冠状窦口中，并且以远端的紧密半径弯曲偏转，以导航在冠状窦分支中发现的短半径，直角转角。 此时，可以将柔性护套放置在引导导管上方，或诸如导线的物体可以插入引导导管的通道中。 该系统由导管和图像采集单元组成，显示正向图像。

公开(公告)号：US06178346B1

公开(公告)日：2001-01-23

申请号：US09178566

申请日：1998-10-23

申请人： David C. Amundson ,  H. John Hanlin

发明人： David C. Amundson ,  H. John Hanlin

IPC分类号： A61B500

CPC分类号： A61B5/0086 ,  A61B1/00165 ,  A61B1/042 ,  A61B1/063 ,  A61B1/0669 ,  A61B1/07 ,  A61B5/0075

摘要： A device and method for imaging an object that is situated within a fluid environment having suspended particles uses infrared illumination. In a representative application, a catheter having fiber optics is inserted into the vasculature of a patient. The fiber optics transmits infrared light to an optical head at a distal end of the catheter, which transmits the light into a bloody environment to an object to be imaged. Light reflecting from the object is collected and transmitted throughout the fiber optic to an infrared camera, so that an image is formed. Proper selection of the wavelength of infrared illumination allows objects to be imaged through what would otherwise be an opaque liquid. The invention has particular utility in the field of medical imaging.

摘要翻译： 用于成像位于具有悬浮颗粒的流体环境内的物体的装置和方法使用红外照明。 在代表性的应用中，将具有光纤的导管插入患者的脉管系统中。 光纤将红外光透射到导管的远端处的光学头，该光头将光传输到血液环境中，使其成像。 从物体反射的光被收集并通过光纤传输到红外相机，从而形成图像。 正确选择红外照明的波长可使物体通过不透明液体进行成像。 本发明在医学成像领域具有特殊的用途。