公开(公告)号：US5618735A

公开(公告)日：1997-04-08

申请号：US405549

申请日：1995-03-16

申请人： Tom Saul ,  Georges Der-Balian ,  Paul Kenney ,  Heidi Mathis ,  Shirley Johnson ,  Hans Ribi ,  Tom Witty

发明人： Tom Saul ,  Georges Der-Balian ,  Paul Kenney ,  Heidi Mathis ,  Shirley Johnson ,  Hans Ribi ,  Tom Witty

IPC分类号： G01N33/53 ,  G01N33/543 ,  G01N33/545 ,  G01N33/58 ,  G01N33/78

CPC分类号： G01N33/54353 ,  G01N33/54306 ,  G01N33/545 ,  G01N33/582 ,  G01N33/78 ,  Y10S435/968 ,  Y10S436/805

摘要： Methods and compositions are provided for the detection of analytes. The method employs a fluorescence production layer which comprises a fluorescent polymerized polyunsaturated lipid layer in association with a ligand which is a member of a specific binding pair, where the ligand is competitive with the analyte for the complementary binding member or is a complementary binding member. By providing for a fluorescence modulation reagent which binds to the fluorescence production layer in proportion to the amount of analyte in the sample, by measuring the resulting fluorescence after carrying out the assay methodology, the amount of analyte can be determined quantitatively.

摘要翻译： 提供了检测分析物的方法和组成。 该方法使用荧光生产层，其包含与作为特异性结合对成员的配体相结合的荧光聚合多不饱和脂质层，其中配体与用于互补结合成员的分析物竞争或是互补结合成员。 通过提供与荧光产生层成比例的荧光调节剂，其与样品中分析物的量成比例，通过在进行测定方法后测量所得到的荧光，可以定量测定分析物的量。