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    UNNATURAL POLYMERASE SUBSTRATES THAT CAN SUSTAIN ENZYMATIC SYNTHESIS OF DOUBLE STRANDED NUCLEIC ACIDS FROM A NUCLEIC ACID TEMPLATE AND METHODS OF USE
    2.
    发明申请
    UNNATURAL POLYMERASE SUBSTRATES THAT CAN SUSTAIN ENZYMATIC SYNTHESIS OF DOUBLE STRANDED NUCLEIC ACIDS FROM A NUCLEIC ACID TEMPLATE AND METHODS OF USE 有权
    可以从核酸模板维持双重拉伸的核酸的酶合成和使用方法的UNNATURAL POLYMERASE底物

    公开(公告)号：US20090269759A1

    公开(公告)日：2009-10-29

    申请号：US12355487

    申请日：2009-01-16

    申请人： Steven M. Menchen, JR. ,  Barnett Rosenblum ,  Paul Kenney ,  Shoeb I. Khan ,  Zhaochun Ma ,  Jer-Kang Chen ,  Joe Lam ,  Boli Huang

    发明人： Steven M. Menchen, JR. ,  Barnett Rosenblum ,  Paul Kenney ,  Shoeb I. Khan ,  Zhaochun Ma ,  Jer-Kang Chen ,  Joe Lam ,  Boli Huang

    IPC分类号： C12Q1/68 ,  C07F9/02

    CPC分类号： C07H21/04 ,  C07H19/073 ,  C07H19/173 ,  C12Q1/6874 ,  C12Q2523/107 ,  C12Q2525/186 ,  C12Q2525/197

    摘要： Nucleotide analogs that can sustain the enzymatic synthesis of double-stranded nucleic acid from a nucleic template are described. The nucleotide analogs include: (i) a base selected from the group consisting of adenine, guanine, cytosine, thymine, uracil and their analogs; (ii) a label attached to the base or analog of the base via a cleavable linker; (iii) a deoxyribose; and (iv) one or more phosphate groups. The linker and/or the label inhibits template directed polymerase incorporation of a further nucleotide substrate onto an extended primer strand. In addition, cleavage of the linker leaves a residue attached to the base which is not present in the natural nucleotide and which does not inhibit extension of the primer strand. The nucleotide analogs can therefore be used as reversible terminators in sequencing by synthesis methods without blocking the 3′ hydroxyl group. Methods of sequencing DNA using the substrates are also described.

    摘要翻译： 描述了可以维持来自核酸模板的双链核酸的酶合成的核苷酸类似物。 核苷酸类似物包括：（i）选自腺嘌呤，鸟嘌呤，胞嘧啶，胸腺嘧啶，尿嘧啶及其类似物的碱基; （ii）通过可切割连接体连接到碱基或类似物的标签; （iii）脱氧核糖; 和（iv）一个或多个磷酸酯基团。 接头和/或标记物将另外的核苷酸底物的模板定向聚合酶掺入到延伸的引物链上。 此外，接头的切割物留下连接到碱基上的残基，其不存在于天然核苷酸中，并且不抑制引物链的延伸。 因此，核苷酸类似物因此可以通过合成方法进行测序而不会阻断3'羟基作为可逆终止子。 还描述了使用底物测序DNA的方法。

    No rake platform
    5.
    发明授权
    No rake platform 有权

    公开(公告)号：US11107322B1

    公开(公告)日：2021-08-31

    申请号：US16739915

    申请日：2020-01-10

    申请人： Paul Kenney

    发明人： Paul Kenney

    IPC分类号： G07F17/32

    摘要： Methods, systems, and apparatus, including computer programs encoded on a computer storage medium, for implementing no rake line wagering. In one aspect, a method includes accessing rake line data for an event. The accessed rake line data is converted into a no rake line. The no rake line is published to a wagering platform accessible by multiple members. The wagering platform includes multiple separate wagering groups that each include multiple members. At a pre-specified time relative to a start time of the sports event, locking any wagers made within a particular group based on the no rake line. An outcome of the sporting event is obtained. From among the wagers, winning wagers and losing wagers in the particular group are determined based on the outcome of the sports event and the no rake line. Accounts of the members in the particular group that wagered on the sporting event are updated.

    Unnatural polymerase substrates that can sustain enzymatic synthesis of double stranded nucleic acids from a nucleic acid template and methods of use
    6.
    发明授权
    Unnatural polymerase substrates that can sustain enzymatic synthesis of double stranded nucleic acids from a nucleic acid template and methods of use 有权
    可以维持来自核酸模板的双链核酸酶的合成和使用方法的非天然聚合酶底物

    公开(公告)号：US08058414B2

    公开(公告)日：2011-11-15

    申请号：US12355487

    申请日：2009-01-16

    申请人： Steven Menchen ,  Barnett Rosenblum ,  Paul Kenney ,  Shoeb Khan ,  Zhaochun Ma ,  Jer-Kang Chen ,  Joe Y. Lam ,  Boli Huang

    发明人： Steven Menchen ,  Barnett Rosenblum ,  Paul Kenney ,  Shoeb Khan ,  Zhaochun Ma ,  Jer-Kang Chen ,  Joe Y. Lam ,  Boli Huang

    IPC分类号： C07G3/00 ,  C07H21/02 ,  C07H21/04 ,  C07H19/04 ,  C12Q1/68 ,  C12P19/34

    CPC分类号： C07H21/04 ,  C07H19/073 ,  C07H19/173 ,  C12Q1/6874 ,  C12Q2523/107 ,  C12Q2525/186 ,  C12Q2525/197

    摘要： Nucleotide analogs that can sustain the enzymatic synthesis of double-stranded nucleic acid from a nucleic template are described. The nucleotide analogs include: (i) a base selected from the group consisting of adenine, guanine, cytosine, thymine, uracil and their analogs; (ii) a label attached to the base or analog of the base via a cleavable linker; (iii) a deoxyribose; and (iv) one or more phosphate groups. The linker and/or the label inhibits template directed polymerase incorporation of a further nucleotide substrate onto an extended primer strand. In addition, cleavage of the linker leaves a residue attached to the base which is not present in the natural nucleotide and which does not inhibit extension of the primer strand. The nucleotide analogs can therefore be used as reversible terminators in sequencing by synthesis methods without blocking the 3′ hydroxyl group. Methods of sequencing DNA using the substrates are also described.

    摘要翻译： 描述了可以维持来自核酸模板的双链核酸的酶合成的核苷酸类似物。 核苷酸类似物包括：（i）选自腺嘌呤，鸟嘌呤，胞嘧啶，胸腺嘧啶，尿嘧啶及其类似物的碱基; （ii）通过可切割连接体连接到碱基或类似物的标签; （iii）脱氧核糖; 和（iv）一个或多个磷酸酯基团。 接头和/或标记物将另外的核苷酸底物的模板定向聚合酶掺入到延伸的引物链上。 此外，接头的切割物留下连接到碱基上的残基，其不存在于天然核苷酸中，并且不抑制引物链的延伸。 因此，核苷酸类似物因此可以通过合成方法进行测序而不会阻断3'羟基作为可逆终止子。 还描述了使用底物测序DNA的方法。