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公开(公告)号：US20110287494A1
公开(公告)日：2011-11-24
申请号：US13060986
申请日：2009-09-01
CPC分类号： C12P41/002 , C12N9/0028 , C12P17/10 , C12R1/465
摘要： Novel enzymes that stereoselectively reduce imine derivatives were isolated and purified, and polynucleotides encoding the enzymes were cloned. Optically active amine derivatives were produced by acting on imine derivatives with a culture of microorganisms having the ability to stereoselectively reduce the compounds, microbial cells or processed products thereof, and/or imine reductases thereof, followed by collecting the generated optically active amine derivatives. The present invention enables, for example, production of an optically active compound represented by formula (IV): (wherein R group represents an alkyl group having one to three carbon atoms; and n represents an integer of 1 to 4).
摘要翻译： 分离和纯化立体选择性还原亚胺衍生物的新型酶，并克隆编码酶的多核苷酸。 通过用具有立体选择性还原化合物，微生物细胞或其加工产物和/或亚胺还原酶的能力的微生物培养物作用于亚胺衍生物，然后收集所产生的光学活性胺衍生物来制备光学活性胺衍生物。 本发明例如可以制备由式（IV）表示的光学活性化合物：其中R基团表示具有一至三个碳原子的烷基，n表示1至4的整数。
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2.发明授权Nucleic acid encoding a polypeptide having aminotransferase activity, vectors and host cells comprising the nucleic acid 有权编码具有氨基转移酶活性的多肽的核酸，载体和包含该核酸的宿主细胞公开(公告)号：US09481870B2
公开(公告)日：2016-11-01
申请号：US13876400
申请日：2011-09-28
申请人： Toru Nagasawa , Toyokazu Yoshida , Kouichi Ishida , Noriyuki Ito , Shigeru Kawano , Yoshihiko Yasohara
发明人： Toru Nagasawa , Toyokazu Yoshida , Kouichi Ishida , Noriyuki Ito , Shigeru Kawano , Yoshihiko Yasohara
CPC分类号： C12N9/1096 , C12P17/10 , C12P41/006 , C12Y206/01 , Y02P20/52
摘要： A method for efficiently producing an optically active amino compound useful as an intermediate for pharmaceutical preparations, agricultural chemicals, or the like, from a ketone compound is provided. Specifically, a polypeptide having high resistance to a water-soluble organic solvent and novel transaminase activity for generating (S)-1-benzyl-3-pyrrolidinone with high optical purity of 93% or more, a gene encoding the same, and a transformant expressing the gene at a high level are also provided herein.
摘要翻译： 提供了从酮化合物有效地制备用作药物制剂，农药等的中间体的光学活性氨基化合物的方法。 具体地说，对水溶性有机溶剂具有高抗性的多肽和具有93％以上的光学纯度的（S）-1-苄基-3-吡咯烷酮的新型转氨酶活性，编码该多肽的基因，以及转化体 本文还提供了表达高水平的基因。
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公开(公告)号：US20130210092A1
公开(公告)日：2013-08-15
申请号：US13876400
申请日：2011-09-28
申请人： Toru Nagasawa , Toyokazu Yoshida , Kouichi Ishida , Noriyuki Ito , Shigeru Kawano , Yoshihiko Yasohara
发明人： Toru Nagasawa , Toyokazu Yoshida , Kouichi Ishida , Noriyuki Ito , Shigeru Kawano , Yoshihiko Yasohara
IPC分类号： C12N9/10
CPC分类号： C12N9/1096 , C12P17/10 , C12P41/006 , C12Y206/01 , Y02P20/52
摘要： A method for efficiently producing an optically active amino compound useful as an intermediate for pharmaceutical preparations, agricultural chemicals, or the like, from a ketone compound is provided. Specifically, a polypeptide having high resistance to a water-soluble organic solvent and novel transaminase activity for generating (S)-1-benzyl-3-pyrrolidinone with high optical purity of 93% or more, a gene encoding the same, and a transformant expressing the gene at a high level are also provided herein.
摘要翻译： 提供了从酮化合物有效地制备用作药物制剂，农药等的中间体的光学活性氨基化合物的方法。 具体地说，对水溶性有机溶剂具有高抗性的多肽和具有93％以上的光学纯度的（S）-1-苄基-3-吡咯烷酮的新型转氨酶活性，编码该多肽的基因，以及转化体 本文还提供了表达高水平的基因。
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