公开(公告)号：US20080305102A1

公开(公告)日：2008-12-11

申请号：US11993774

申请日：2006-06-23

申请人： Toshihiro Nakajima ,  Yukihiro Kato ,  Tadayuki Yamadera ,  Tetsuya Amano

发明人： Toshihiro Nakajima ,  Yukihiro Kato ,  Tadayuki Yamadera ,  Tetsuya Amano

IPC分类号： A61K39/395 ,  C07H21/00 ,  C07K16/18 ,  C07K2/00 ,  C12Q1/68 ,  G01N33/50 ,  A61K31/53 ,  C07D403/04 ,  C07D251/54 ,  A61P35/00 ,  A61K38/00 ,  A61K48/00

CPC分类号： G01N33/5011 ,  A01K2267/0325 ,  A61K31/53 ,  A61K31/7088 ,  A61K38/1709 ,  A61K38/53 ,  C12N9/93 ,  C12N15/1135 ,  C12N2310/11 ,  C12N2310/14 ,  G01N2500/00 ,  G01N2800/102

摘要： Inhibition of synoviolin function was found to activate the cancer-suppressing protein p53. Substances inhibiting the function of synoviolin are useful as cancer therapeutic agents. The inhibition of synoviolin function was also found to lead to the inhibition of p53 ubiquitination, increased activity of p53 phosphorylation proteins, and the like. Based on these findings, the present invention provides methods capable of efficiently screening for cancer therapeutic agents. Further, it was also found that regulation of the autoubiquitination of synoviolin protein suppresses the proliferation of rheumatoid arthritis synovial cells. Substances regulating the autoubiquitination of synoviolin protein are useful as anti-rheumatic agents. Moreover, the present invention provides methods of efficiently screening for anti-rheumatic agents.

摘要翻译： 发现滑膜蛋白功能的抑制活化了癌症抑制蛋白p53。 抑制滑膜素功能的物质可用作癌症治疗剂。 还发现滑膜蛋白功能的抑制导致p53泛素化的抑制，p53磷酸化蛋白的活性增加等。 基于这些发现，本发明提供能够有效筛选癌症治疗剂的方法。 此外，还发现调节滑膜蛋白自身泛素化抑制类风湿性关节炎滑膜细胞的增殖。 调节滑膜蛋白自身泛素化的物质可用作抗风湿药。 此外，本发明提供了有效筛选抗风湿剂的方法。

公开(公告)号：US20080274479A1

公开(公告)日：2008-11-06

申请号：US11628412

申请日：2005-06-02

申请人： Toshihiro Nakajima ,  Sayumi Toriyama ,  Tadayuki Yamadera ,  Tomohiko Ohta ,  Satoshi Yamasaki ,  Naoko Yagishita ,  Lei Zhang ,  Rie Ikeda ,  Takeshi Sasaki ,  Tetsuya Amano

发明人： Toshihiro Nakajima ,  Sayumi Toriyama ,  Tadayuki Yamadera ,  Tomohiko Ohta ,  Satoshi Yamasaki ,  Naoko Yagishita ,  Lei Zhang ,  Rie Ikeda ,  Takeshi Sasaki ,  Tetsuya Amano

IPC分类号： G01N33/53

CPC分类号： C12Q1/25 ,  G01N2333/9015 ,  G01N2500/02

摘要： The present invention relates to an assay method for determining a auto-ubiquitination activity of synoviolin, comprising reacting synoviolin and ubiquitin in a reaction system containing them and determining an amount of ubiquitin binding to synoviolin, to a method of screening a substance capable of regulating such an activity, and to a kit for auto-ubiquitination assay of synoviolin.

摘要翻译： 本发明涉及一种用于测定滑膜蛋白的自身泛素化活性的测定方法，包括使滑膜蛋白和泛素在含有它们的反应体系中反应并确定与滑膜蛋白结合的泛素的量，以筛选能够调节这种蛋白质的物质的方法 一种活性，以及​​用于滑膜蛋白自动泛素化测定的试剂盒。