公开(公告)号：US20180208999A1

公开(公告)日：2018-07-26

申请号：US15880403

申请日：2018-01-25

申请人： The Chinese University of Hong Kong Office of Research and Knowledge Transfer Services ,  GRAIL, Inc.

发明人： Yuk-Ming Dennis Lo ,  Rossa Wai Kwun Chiu ,  Kwan Chee Chan ,  Peiyong Jiang ,  Wai Kei Lam

IPC分类号： C12Q1/6888 ,  C12Q1/6879 ,  C12Q1/6806 ,  C12Q1/6886 ,  C12Q1/686 ,  G16H10/40 ,  G16H50/70 ,  G16H50/20

CPC分类号： C12Q1/6888 ,  C12Q1/6806 ,  C12Q1/686 ,  C12Q1/6879 ,  C12Q1/6886 ,  C12Q2600/112 ,  C12Q2600/118 ,  C12Q2600/156 ,  C12Q2600/158 ,  C12Q2600/166 ,  G16B20/00 ,  G16B30/10 ,  G16B40/00 ,  G16H10/40 ,  G16H50/20 ,  G16H50/30 ,  G16H50/70 ,  Y02A90/26

摘要： Various embodiments are directed to applications (e.g., classification of biological samples) of the analysis of the count, the fragmentation patterns, and size of cell-free nucleic acids, e.g., plasma DNA and serum DNA, including nucleic acids from pathogens, such as viruses. Embodiments of one application can determine if a subject has a particular condition. For example, a method of present disclosure can determine if a subject has cancer or a tumor, or other pathology. Embodiments of another application can be used to assess the stage of a condition, or the progression of a condition over time. For example, a method of the present disclosure may be used to determine a stage of cancer in a subject, or the progression of cancer in a subject over time (e.g., using samples obtained from a subject at different times).

公开(公告)号：US20180142299A1

公开(公告)日：2018-05-24

申请号：US15816263

申请日：2017-11-17

申请人： SeraCare Life Sciences,Inc

发明人： Yves Konigshofer

IPC分类号： C12Q1/6883 ,  C12Q1/6855 ,  C12N15/10 ,  C12Q1/6879

CPC分类号： C12Q1/6883 ,  C12N15/1093 ,  C12Q1/6827 ,  C12Q1/6855 ,  C12Q1/6879 ,  C12Q2600/166 ,  C12Q2525/191 ,  C12Q2563/179

摘要： Disclosed are controls and reference materials for use in identifying any of a number of genotypes and/or for use in identifying or characterizing a disease or condition. The controls and reference materials may be particularly useful for diagnostic tests that utilize circulating cell-free DNA (cfDNA). Also disclosed herein are methods of generating large quantities of cfDNA that preserve the original cfDNA size and input sequences. In some embodiments, the methods comprise amplifying, digesting, and purifying cfDNA isolated from a subject. The cfDNA may be circulating tumor DNA (“ctDNA). The methods disclosed herein result in a cfDNA or ctDNA clone library that is significantly more “commutable” compared to existing methods.

公开(公告)号：US09328385B2

公开(公告)日：2016-05-03

申请号：US14000092

申请日：2012-02-20

申请人： Francesca Di Pasquale ,  Holger Engel ,  Sascha Strauss ,  Nicola Jo Thelwell

发明人： Francesca Di Pasquale ,  Holger Engel ,  Sascha Strauss ,  Nicola Jo Thelwell

IPC分类号： C12P19/34 ,  C12Q1/68

CPC分类号： C12Q1/6879 ,  C12Q1/6851 ,  C12Q1/6888 ,  C12Q2600/16 ,  C12Q2600/166 ,  C12Q2525/151 ,  C12Q2545/101 ,  C12Q2561/113

摘要： The present invention relates to a method for quantifying and/or detecting one or more nucleic acids of a genome in a sample, wherein in an amplification reaction, (i) a first nucleic acid is amplified, the locus that is amplified is a multicopy locus (MCL) within the genome, wherein the locus shares at least 80% sequence identity to a sequence according to SEQ ID NO. 1 over a stretch of 80 base pairs, and wherein the multicopy locus has copies on at least two different chromosomes, (ii) a second nucleic acid that has been added as an internal control (IC) is also amplified, and (iii) the amount of amplification product from the amplification of the first nucleic acid is determined.

摘要翻译： 本发明涉及用于量化和/或检测样品中基因组的一种或多种核酸的方法，其中在扩增反应中，（i）第一个核酸被扩增，被扩增的基因座是多拷贝基因座 （MCL），其中所述基因座与SEQ ID NO：1的序列具有至少80％的序列同一性。 并且其中多拷贝基因座在至少两个不同染色体上具有拷贝，（ii）作为内部对照（IC）加入的第二个核酸也被扩增，并且（iii） 确定来自扩增第一核酸的扩增产物的量。

公开(公告)号：US20150203811A1

公开(公告)日：2015-07-23

申请号：US14472155

申请日：2014-08-28

申请人： BIOCERN, INC.

发明人： DAVID OKSENBERG ,  SERGEY KRYLOV ,  MICHAEL MUSHEEV

IPC分类号： C12N5/076 ,  C12N5/071

CPC分类号： C12Q1/6811 ,  C12N5/061 ,  C12N5/0612 ,  C12N15/115 ,  C12N2310/16 ,  C12N2320/10 ,  C12Q1/6879

摘要： The present invention provides an aptamer or pool of aptamers (nucleic acid sequences) that bind(s) to a target molecule on the surface, accessible from the surface or inside of a mammalian sperm cell and a method for producing the aptamers. The method comprises contacting a collection of different nucleic acid molecules with the target molecule under conditions favorable for binding between at least one of the nucleic acid molecules and the target molecule, to form at least one complex comprising the nucleic acid molecule bound to the target molecule, wherein each of the nucleic acid molecules comprises at least one segment of randomized nucleotide sequences. The complexes are then separated from the unbound nucleic acid molecules and unbound target molecules, and the bound nucleic acid molecule is recovered from the separated complex. The aptamers are used to separate sperm cells based upon sperm cell qualities, such as whether the cells contain X chromosomes or Y chromosomes.

公开(公告)号：US20150133332A1

公开(公告)日：2015-05-14

申请号：US14464749

申请日：2014-08-21

申请人： KellBenx, Inc.

发明人： Javad Khosravi ,  Leonard H. Kellner ,  Hassan Bennani

IPC分类号： G01N33/80

CPC分类号： G01N33/80 ,  C12Q1/6806 ,  C12Q1/6879 ,  G01N33/56966 ,  G01N33/57492 ,  G01N2333/805 ,  C12Q2563/131 ,  C12Q2563/149

摘要： The invention provides a method for isolating or enriching a rare cell from a biological fluid of a mammal employing an antibody that binds a cell-surface antigen of the rare cell. The immobilized antibody is incubated with a sample of biological fluid that includes the rare cells and a plurality of other cells so as to form an antibody-rare cell complex. The complex can be detected or isolated and subsequently analyzed by any of a variety of physical, chemical and genetic techniques.

摘要翻译： 本发明提供了使用结合稀有细胞的细胞表面抗原的抗体从哺乳动物的生物流体中分离或富集稀有细胞的方法。 将固定的抗体与包含罕见细胞和多个其他细胞的生物流体样品一起孵育，形成抗体稀有细胞复合体。 可以检测或分离复合物，并随后通过各种物理，化学和遗传技术中的任何一种进行分析。

公开(公告)号：US08709726B2

公开(公告)日：2014-04-29

申请号：US12401493

申请日：2009-03-10

申请人： Paul Andrew Oeth ,  Payam Mahboubi ,  Min Seob Lee

发明人： Paul Andrew Oeth ,  Payam Mahboubi ,  Min Seob Lee

IPC分类号： C12Q1/68 ,  C12P19/34 ,  C07H21/04

CPC分类号： C12Q1/6879 ,  C12Q2600/16

摘要： Provided herein are compositions, processes and kits for noninvasive, early determination of fetal sex from, and/or amount of fetal nucleic acid in, an extracellular nucleic acid sample from a pregnant female. Such compositions, processes and kits are useful for detection of low genomic copy numbers of male fetal nucleic acid in a high copy number background of female nucleic acid, thereby determining the sex of a fetus and/or amount of fetal nucleic acid in a sample.

摘要翻译： 本文提供了从怀孕女性的细胞外核酸样品中非侵入性早期测定胎儿性别和/或胎儿核酸量的组合物，方法和试剂盒。 这样的组合物，方法和试剂盒可用于检测雌性核酸的高拷贝数背景下的雄性胎儿核酸的低基因组拷贝数，从而确定样品中胎儿的性别和/或胎儿核酸的量。

公开(公告)号：US20130203108A1

公开(公告)日：2013-08-08

申请号：US13752057

申请日：2013-01-28

申请人： XY, LLC

发明人： Kenneth M. Evans ,  Erik B. van Munster

IPC分类号： C12N5/076

CPC分类号： C12Q1/6879 ,  A61D19/00 ,  B03B9/00 ,  C12N5/061 ,  C12N5/0612 ,  G01F17/00 ,  G01N15/14 ,  G01N15/147 ,  G01N21/17 ,  G01N2015/149

摘要： Isolated non-naturally occurring populations of spermatozoa (15) having high purity and technologies to differentiate spermatozoa (28) based on characteristics such as mass, volume, orientation, or emitted light including methods of analysis and apparatus such as beam shaping optics (30) and detectors (32).

公开(公告)号：US08460872B2

公开(公告)日：2013-06-11

申请号：US13458341

申请日：2012-04-27

申请人： Anders Nygren

发明人： Anders Nygren

IPC分类号： C12Q1/68

CPC分类号： C12Q1/6851 ,  C12Q1/6879 ,  C12Q1/6881 ,  C12Q1/6883 ,  C12Q2600/118 ,  C12Q2600/154 ,  C12Q2600/16 ,  C12Q2600/166 ,  C12Q2521/331 ,  C12Q2525/204 ,  C12Q2537/16 ,  C12Q2545/107

摘要： The technology relates in part to quantification of a minority nucleic acid species from a nucleic acid sample. In some embodiments, methods for determining the amount of fetal nucleic acid (e.g. absolute amount, relative amount) in a maternal sample are provided.

摘要翻译： 该技术部分涉及从核酸样品中定量少数核酸物种。 在一些实施方案中，提供了用于确定母体样品中胎儿核酸量（例如绝对量，相对量）的方法。

公开(公告)号：US08450061B2

公开(公告)日：2013-05-28

申请号：US13457978

申请日：2012-04-27

申请人： Anders Nygren

发明人： Anders Nygren

IPC分类号： C12Q1/68

CPC分类号： C12Q1/6851 ,  C12Q1/6879 ,  C12Q1/6881 ,  C12Q1/6883 ,  C12Q2600/118 ,  C12Q2600/154 ,  C12Q2600/16 ,  C12Q2600/166 ,  C12Q2521/331 ,  C12Q2525/204 ,  C12Q2537/16 ,  C12Q2545/107

摘要： The technology relates in part to quantification of a minority nucleic acid species from a nucleic acid sample. In some embodiments, methods for determining the amount of fetal nucleic acid (e.g. absolute amount, relative amount) in a maternal sample are provided.

公开(公告)号：US20130122492A1

公开(公告)日：2013-05-16

申请号：US13295532

申请日：2011-11-14

申请人： Javad Khosravi ,  Leonard H. Kellner ,  Hassan Benanni

发明人： Javad Khosravi ,  Leonard H. Kellner ,  Hassan Benanni

IPC分类号： C12Q1/68 ,  G01N33/566 ,  C12N11/02

CPC分类号： G01N33/80 ,  C12Q1/6806 ,  C12Q1/6879 ,  G01N33/56966 ,  G01N33/57492 ,  G01N2333/805 ,  C12Q2563/131 ,  C12Q2563/149

摘要： The invention provides a method for isolating or enriching a rare cell from a biological fluid of a mammal employing an antibody that binds a cell-surface antigen of the rare cell. The immobilized antibody is incubated with a sample of biological fluid that includes the rare cells and a plurality of other cells so as to form an antibody-rare cell complex. The complex can be detected or isolated and subsequently analyzed by any of a variety of physical, chemical and genetic techniques.