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    Amylase inhibitors
    1.
    发明授权
    Amylase inhibitors 失效
    淀粉酶抑制剂

    公开(公告)号：US5726291A

    公开(公告)日：1998-03-10

    申请号：US782176

    申请日：1997-01-14

    申请人： Toshiyuki Miyazaki ,  Toshihisa Morimoto ,  Ryuji Murayama

    发明人： Toshiyuki Miyazaki ,  Toshihisa Morimoto ,  Ryuji Murayama

    IPC分类号： A23L1/30 ,  A23L1/305 ,  A23L1/307 ,  A61K38/00 ,  A61K38/55 ,  A61P3/04 ,  A61P3/06 ,  A61P3/08 ,  A61P3/10 ,  A61P43/00 ,  C07K14/415 ,  C07K3/02 ,  A61K38/16 ,  C07K3/24 ,  C07K15/10

    CPC分类号： C07K14/415 ,  A23L33/17 ,  A61K38/00

    摘要： An amylase inhibitor consisting of a protein constructed of 244 amino acid residues having two subunits each identified as SEQ ID NO:1, in which a single band is observed at a mobility of 0.26 by polyacrylamide gel electrophoresis, and having a high inhibitory activity against human pancreatic .alpha.-amylase to inhibit an increase in blood glucose level, control an insulin secretion or maintain the duration in a feeling of satiety for diet. The amylase inhibitor can be used alone or in combination with other known amylase inhibitors.

    摘要翻译： 由由244个氨基酸残基构成的蛋白质组成的淀粉酶抑制剂，其具有两个各自鉴定为SEQ ID NO：1的亚基，其中通过聚丙烯酰胺凝胶电泳以0.26的迁移率观察到单个条带，并且对人体具有高的抑制活性 胰腺α-淀粉酶以抑制血糖水平的升高，控制胰岛素分泌或维持持续时间对于饮食的饱腹感。 淀粉酶抑制剂可以单独使用或与其它已知的淀粉酶抑制剂组合使用。

    Amylase inhibitors
    3.
    发明授权
    Amylase inhibitors 失效
    淀粉酶抑制剂

    公开(公告)号：US5444046A

    公开(公告)日：1995-08-22

    申请号：US216846

    申请日：1994-03-24

    申请人： Toshiyuki Miyazaki ,  Toshihisa Morimoto ,  Ryuji Murayama ,  Hiroshi Matsubara

    发明人： Toshiyuki Miyazaki ,  Toshihisa Morimoto ,  Ryuji Murayama ,  Hiroshi Matsubara

    IPC分类号： A23J1/12 ,  A23J3/18 ,  A61K38/00 ,  C07K14/415 ,  A61K38/16 ,  C07K1/18

    CPC分类号： C07K14/415 ,  A23J1/12 ,  A23J3/18 ,  A61K38/00

    摘要： An amylase inhibitor consisting essentially of a protein constructed of 248 amino acid residues having two subunits, each identified as SEQ ID NO:1, in which a single band is observed at a mobility of 0.26 by polyacrylamide gel electrophoresis. The amylase inhibitor can be extracted from wheat and purified by absorption on a cation exchange resin. It is useful for inhibiting an increase in blood glucose level, controlling insulin secretion, suppressing appetite, and as a food additive. The new amylase inhibitor can be used in combination with a protein composed of two subunits, each identified as SEQ ID NO:2, the total content of both proteins being not less than 20% by weight.

    摘要翻译： 淀粉酶抑制剂基本上由具有248个氨基酸残基构成的蛋白质组成，其具有两个亚基，每个被鉴定为SEQ ID NO：1，其中通过聚丙烯酰胺凝胶电泳以0.26的迁移率观察到单个条带。 可以从小麦中提取淀粉酶抑制剂，并通过在阳离子交换树脂上吸收进行纯化。 抑制血糖水平升高，抑制胰岛素分泌，抑制食欲，以及作为食品添加剂是有用的。 新的淀粉酶抑制剂可与由两个亚基组成的蛋白质组合使用，每个亚基各自鉴定为SEQ ID NO：2，两种蛋白质的总含量不小于20重量％。

    Processes for the preparation of amylase inhibitor
    5.
    发明授权
    Processes for the preparation of amylase inhibitor 失效
    制备淀粉酶抑制剂的方法

    公开(公告)号：US5332803A

    公开(公告)日：1994-07-26

    申请号：US49180

    申请日：1993-04-19

    申请人： Toshiyuki Miyazaki ,  Ryuji Murayama ,  Toshihisa Morimoto

    发明人： Toshiyuki Miyazaki ,  Ryuji Murayama ,  Toshihisa Morimoto

    IPC分类号： A61K38/55 ,  A23L29/00 ,  A61K38/00 ,  A61P3/08 ,  A61P3/10 ,  C07K1/00 ,  C07K1/14 ,  C07K1/18 ,  C07K1/32 ,  C07K14/415 ,  C12N9/99 ,  C07K3/02 ,  C07K3/24 ,  C07K3/28 ,  C07K15/10

    CPC分类号： C07K14/415 ,  A23L29/03 ,  A61K38/005

    摘要： A process for the preparation of an amylase inhibitor is disclosed which comprises the steps of:(a) extracting wheat, wheat flour or wheat gluten with water, a dilute acid, a dilute alkali or an aqueous alcohol to produce a solution containing the amylase inhibitor;(b) adding a polysaccharide to said solution to form an insoluble complex of the amylase inhibitor with the polysaccharide and separating the insoluble complex from the solution;(c) dissolving or dispersing said complex in a solution, then separating the polysaccharide from the solution to collect a solution containing the amylase inhibitor; and(d) treating the collected solution with a cation exchanger to recover the amylase inhibitor from fractions that have not been adsorbed on the cation exchanger.The amylase inhibitors produced by the above process possess a high amylase inhibitory activity but substantially no trypsin inhibitory activity, and are useful in the prophylaxis and treatment of diseases such as hyperglycemia, diabetes, hyperlipemia, arteriosclerosis and obesity.

    摘要翻译： 公开了一种制备淀粉酶抑制剂的方法，其包括以下步骤：（a）用水，稀酸，稀碱或含水醇提取小麦，小麦粉或小麦面筋以产生含有淀粉酶抑制剂的溶液 ; （b）向所述溶液中加入多糖以形成淀粉酶抑制剂与多糖的不溶性络合物，并从溶液中分离不溶性络合物; （c）将所述复合物溶解或分散在溶液中，然后从溶液中分离多糖以收集含有淀粉酶抑制剂的溶液; 和（d）用阳离子交换剂处理收集的溶液，以从未吸附在阳离子交换器上的级分回收淀粉酶抑制剂。 通过上述方法制备的淀粉酶抑制剂具有高淀粉酶抑制活性，但基本上没有胰蛋白酶抑制活性，并且可用于预防和治疗高血糖症，糖尿病，高脂血症，动脉硬化和肥胖症等疾病。