公开(公告)号：US20210353556A1

公开(公告)日：2021-11-18

申请号：US17320946

申请日：2021-05-14

Applicant: Translate Bio, Inc.

Inventor： Shrirang Karve ,  Frank DeRosa ,  Natalia Vargas Montoya ,  Asad Khanmohammed

IPC: A61K9/51 ,  A61K31/713

Abstract: The present invention provides, among other things, methods of encapsulating messenger RNA in lipid nanoparticles without the use of flammable solvents, and compositions produced by these methods, for mRNA delivery in therapeutic use. The present invention is, in part, based on the surprising discovery that mRNA can be encapsulated with high efficiency, without using an ethanol solvent, in the presence of an amphiphilic polymer. Thus, the present invention provides safe, cost-effective, and efficient methods of producing LNP formulations from large scale manufacturing processes as well as in low volume formulations for therapeutic applications.

公开(公告)号：US20220218612A1

公开(公告)日：2022-07-14

申请号：US17611020

申请日：2020-05-14

Applicant: Translate Bio, Inc.

Inventor： Shrirang Karve ,  Frank DeRosa ,  Michael Hearlein ,  Ashish Sarode ,  Zarna Patel ,  Rebecca L. Ball ,  Natalia Vargas Montoya ,  Priyal Patel ,  Asad Khanmohammed

IPC: A61K9/127 ,  A61K9/00 ,  A61K31/7088 ,  A61K9/16

Abstract: The present invention provides an improved process for lipid nanoparticle formulation and mRNA encapsulation. In some embodiments, the present invention provides a process for enhanced encapsulation of messenger RNA (mRNA) in lipid nanoparticles comprising a step of heating the mRNA-encapsulated lipid nanoparticles in a drug product formulation solution.