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1.
公开(公告)号:US20220112471A1
公开(公告)日:2022-04-14
申请号:US17277661
申请日:2019-08-20
Applicant: TSINGHUA UNIVERSITY
Abstract: Disclosed is an NADH-dependent amino acid dehydrogenase and an application thereof in increasing lysine yield. The amino acid dehydrogenases are aspartate dehydrogenase derived from Pseudomonas aeruginos, aspartate semialdehyde dehydrogenase derived from Tistrella mobilis, dihydropyridine dicarboxylic acid reductase derived from Mycobacterium tuberculosis, and diaminopimelate dehydrogenase derived from Tepidanaerobacter acetatoxydans. The amino acid sequences thereof are as shown in SEQ ID NOs: 1, 3, 5, and 7, respectively. NADH or both NADH and NADPH can be used as co-factors of the amino acid dehydrogenase to synthesize lysine, thereby reducing the demand for NADPH in the cell, and significantly increasing the production of lysine or pentanediamine.
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2.
公开(公告)号:US20230391716A1
公开(公告)日:2023-12-07
申请号:US18035625
申请日:2022-01-07
Applicant: Tsinghua University
IPC: C07C273/16 , B01D53/78 , B01D53/56
CPC classification number: C07C273/16 , B01D53/78 , B01D53/56 , B01D2258/0283
Abstract: The present disclosure relates to a method and a system for preparing urea by coupling denitration with electrocatalytic reduction. Specifically, the method of the present disclosure comprises: introducing an absorption solution containing nitrite and nitrate, which is generated by subjecting flue gas containing nitric oxide to a denitration step, into a separation step to separate the nitrite and the nitrate, thereby obtaining a solution rich in the nitrite and the nitrate; and introducing the solution rich in the nitrite and the nitrate generated in the separation step into an electrocatalysis step, and carrying out the electrocatalytic reduction in the presence of carbon dioxide or carbonate ions to generate a urea solution. The system of the present disclosure comprises: a denitration device, a separation device connected to the denitration device, and an electrocatalytic device connected to the separation device. The method and system of the present disclosure can be used for the deep removal of nitrogen oxides from industrial flue gas at a low temperature, and can achieve the recycling of nitrogen.
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