公开(公告)号：US06207378B1

公开(公告)日：2001-03-27

申请号：US09136411

申请日：1998-08-19

申请人： Tsuneo Yamane ,  Hideo Nakano ,  Masashi Ouchi ,  Reiko Okumura ,  Satoshi Sekiguchi

发明人： Tsuneo Yamane ,  Hideo Nakano ,  Masashi Ouchi ,  Reiko Okumura ,  Satoshi Sekiguchi

IPC分类号： C12Q168

CPC分类号： C12P21/02 ,  C12Q1/686 ,  C12Q2527/143

摘要： A method for amplifying desired nucleic acid molecules by PCR which comprises the steps of isolating and purifying a group of nucleic acid molecules including desired nucleic acid molecules to be amplified, then carrying out PCR while establishing such a condition that the nucleic acid molecules capable of being amplified, except for primers, present in a PCR reaction solution are constituted by only the desired nucleic acid molecules and that the concentration of each primer used is limited to a level of not more than 100 nM; a method for producing a protein in a cell-free protein-synthesis system containing a cell-free extract which comprises using the nucleic acid of a single kind produced by the method described above and a method for establishing a protein library which comprises the steps of separately carrying out the method for amplifying desired nucleic acid molecules by PCR to obtain at least two kinds of nucleic acid molecules and separately carrying out the foregoing method for producing a protein using each of the at least two kinds of the amplified nucleic acids as a template to thus establish a protein library which comprises at least two kinds of proteins encoded by the resulting at least two nucleic acids respectively.

摘要翻译： 一种通过PCR扩增所需核酸分子的方法，该方法包括以下步骤：分离和纯化包括要扩增的所需核酸分子的核酸分子组，然后进行PCR，同时建立这样的条件，使得核酸分子能够 存在于PCR反应溶液中的引物除了扩增，仅由所需的核酸分子构成，使用的引物的浓度限制在不超过100nM的水平; 一种含有无细胞提取物的无细胞蛋白质合成系统中的蛋白质的制备方法，该方法包括使用通过上述方法制备的单一种核酸和建立蛋白质文库的方法，该方法包括以下步骤： 分别进行通过PCR扩增所需核酸分子的方法，以获得至少两种核酸分子，并且使用所述至少两种扩增的核酸作为模板分别进行上述制备蛋白质的方法 从而建立蛋白质文库，该蛋白质文库分别包含由所得至少两个核酸编码的至少两种蛋白质。

公开(公告)号：US20130345401A1

公开(公告)日：2013-12-26

申请号：US14002430

申请日：2012-03-02

申请人： Satoshi Sekiguchi ,  Manabu Takahisa ,  Masahiro Tomita

发明人： Satoshi Sekiguchi ,  Manabu Takahisa ,  Masahiro Tomita

IPC分类号： C07K14/75

CPC分类号： C07K14/75 ,  A01K67/04 ,  A01K2217/052 ,  A01K2227/706 ,  A01K2267/01

摘要： Disclosed is a novel means that enables mass production of highly safe fibrinogen at low cost. The transgenic silkworm of the present invention expresses the fibrinogen subunit Aα, Bβ and γ chains in the silk gland cells and produces fibrinogen having coagulation activity in the cocoon filament. Preferably, the transgenic silkworm expresses the subunits in the middle silk gland cells and produces fibrinogen in the sericin layer of the cocoon filament. By recovering fibrinogen from the cocoon of the transgenic silkworm of the present invention, highly safe fibrinogen can be mass-produced at low cost.

摘要翻译： 公开了一种能够以低成本大量生产高度安全的纤维蛋白原的新颖手段。 本发明的转基因蚕在丝腺细胞中表达纤维蛋白原亚基Aalpha，Bbeta和γ链，并产生在茧丝中具有凝结活性的纤维蛋白原。 优选地，转基因蚕在中间丝腺细胞中表达亚单位，并在茧丝的丝胶蛋白层中产生纤维蛋白原。 通过从本发明的转基因蚕的茧回收纤维蛋白原，可以低成本大量生产高度安全的纤维蛋白原。

公开(公告)号：US09447167B2

公开(公告)日：2016-09-20

申请号：US14002430

申请日：2012-03-02

申请人： Satoshi Sekiguchi ,  Manabu Takahisa ,  Masahiro Tomita

发明人： Satoshi Sekiguchi ,  Manabu Takahisa ,  Masahiro Tomita

IPC分类号： C12N15/00 ,  C07K14/75 ,  A01K67/04

CPC分类号： C07K14/75 ,  A01K67/04 ,  A01K2217/052 ,  A01K2227/706 ,  A01K2267/01

摘要： Disclosed is a novel means that enables mass production of highly safe fibrinogen at low cost. The transgenic silkworm of the present invention expresses the fibrinogen subunit Aα, Bβ and γ chains in the silk gland cells and produces fibrinogen having coagulation activity in the cocoon filament. Preferably, the transgenic silkworm expresses the subunits in the middle silk gland cells and produces fibrinogen in the sericin layer of the cocoon filament. By recovering fibrinogen from the cocoon of the transgenic silkworm of the present invention, highly safe fibrinogen can be mass-produced at low cost.

摘要翻译： 公开了一种能够以低成本大量生产高度安全的纤维蛋白原的新颖手段。 本发明的转基因蚕在丝腺细胞中表达纤维蛋白原亚基Aα，Bβ和γ链，并产生在茧丝中具有凝血活性的纤维蛋白原。 优选地，转基因蚕在中间丝腺细胞中表达亚单位，并在茧丝的丝胶蛋白层中产生纤维蛋白原。 通过从本发明的转基因蚕的茧回收纤维蛋白原，可以低成本大量生产高度安全的纤维蛋白原。

公开(公告)号：US20080074983A1

公开(公告)日：2008-03-27

申请号：US11862083

申请日：2007-09-26

申请人： Ryoichi Kawasaki ,  Satoshi Sekiguchi

发明人： Ryoichi Kawasaki ,  Satoshi Sekiguchi

IPC分类号： G11B7/00

CPC分类号： G11B7/0903 ,  G11B7/1353 ,  G11B2007/0013

摘要： An optical pickup device for reading a signal recorded in an optical disc including a first signal recording layer and a second signal recording layer, comprising: a laser diode configured to emit laser light; and a diffraction grating configured to be applied with laser light emitted from the laser diode, and to generate a main beam including zero order light, and a sub beam including plus first order light and minus first order light, such that a ratio of light amount of the sub beam to that of the main beam is substantially 1:7.

摘要翻译： 一种用于读取记录在包括第一信号记录层和第二信号记录层的光盘中的信号的光学拾取装置，包括：被配置为发射激光的激光二极管; 以及衍射光栅，被配置为施加从激光二极管发射的激光，并且产生包括零级光的主光束和包括正一级光和负一级光的子光束，使得光量比 的子光束大致为1：7。