公开(公告)号：US20120231536A1

公开(公告)日：2012-09-13

申请号：US13046744

申请日：2011-03-13

申请人： Tzong-Yuan WU ,  Ming-Kun Liu

发明人： Tzong-Yuan WU ,  Ming-Kun Liu

IPC分类号： C12N15/63 ,  C07H21/04

CPC分类号： C12N15/86 ,  C12N2710/14143 ,  C12N2840/203

摘要： An improved baculovirus vector capable of expressing genes in mammalian or insect host cells, and the uses thereof are disclosed. The improved baculovirus vector includes in sequence: a promoter; a first nucleic acid operably linked to the promoter for expressing a first protein in the mammalian or insect host cells; a chimera internal ribosomal entry site (IRES) comprising a portion of an enterovirus (EV) IRES sequence at least 90% identical to SEQ ID NO: 1 and a portion of a Rhopalosiphum padi virus (RhPV) IRES sequence at least 90% identical to SEQ ID NO: 2; and a second nucleic acid operable linked to the portion of the RhPV IRES sequence for expressing a second protein in the mammalian or insect host cells.

摘要翻译： 公开了能够在哺乳动物或昆虫宿主细胞中表达基因的改良的杆状病毒载体及其用途。 改良的杆状病毒载体依次包括：启动子; 与所述启动子可操作地连接以在哺乳动物或昆虫宿主细胞中表达第一蛋白质的第一核酸; 嵌合体内部核糖体进入位点（IRES），其包含与SEQ ID NO：1至少90％相同的一部分肠病毒（EV）IRES序列和一部分与Rhopalosiphum padi病毒（RhPV）IRES序列至少90％相同的 SEQ ID NO：2; 以及可操作地连接到所述RhPV IRES序列的部分以在哺乳动物或昆虫宿主细胞中表达第二蛋白质的第二核酸。

公开(公告)号：US08367404B2

公开(公告)日：2013-02-05

申请号：US13046744

申请日：2011-03-13

申请人： Tzong-Yuan Wu ,  Ming-Kun Liu

发明人： Tzong-Yuan Wu ,  Ming-Kun Liu

IPC分类号： C12N15/00 ,  C07H21/04 ,  C12N5/00

CPC分类号： C12N15/86 ,  C12N2710/14143 ,  C12N2840/203

摘要： An improved baculovirus vector capable of expressing genes in mammalian or insect host cells, and the uses thereof are disclosed. The improved baculovirus vector includes in sequence: a promoter; a first nucleic acid operably linked to the promoter for expressing a first protein in the mammalian or insect host cells; a chimera internal ribosomal entry site (IRES) comprising a portion of an enterovirus (EV) IRES sequence at least 90% identical to SEQ ID NO: 1 and a portion of a Rhopalosiphum padi virus (RhPV) IRES sequence at least 90% identical to SEQ ID NO: 2; and a second nucleic acid operable linked to the portion of the RhPV IRES sequence for expressing a second protein in the mammalian or insect host cells.

摘要翻译： 公开了能够在哺乳动物或昆虫宿主细胞中表达基因的改良的杆状病毒载体及其用途。 改良的杆状病毒载体依次包括：启动子; 与所述启动子可操作地连接以在哺乳动物或昆虫宿主细胞中表达第一蛋白质的第一核酸; 嵌合体内部核糖体进入位点（IRES），其包含与SEQ ID NO：1至少90％相同的一部分肠病毒（EV）IRES序列和一部分与Rhopalosiphum padi病毒（RhPV）IRES序列至少90％相同的 SEQ ID NO：2; 以及可操作地连接到所述RhPV IRES序列的部分以在哺乳动物或昆虫宿主细胞中表达第二蛋白质的第二核酸。

公开(公告)号：US07303704B2

公开(公告)日：2007-12-04

申请号：US10388584

申请日：2003-03-17

申请人： Cheng-Tsung Chang ,  Ming-Kun Liu

发明人： Cheng-Tsung Chang ,  Ming-Kun Liu

IPC分类号： C04B400/00 ,  C04B35/00

CPC分类号： C03C11/007

摘要： The present invention discloses a method of manufacturing lightweight ceramics by mixing several raw materials such as a powdered glass material, a powdered alkali silicate, and a water-soluble polymer in a certain proportion to form a dry substrate, and then adding water to mold the substrate into a desired shape; after the water in the substrate is baked dry, heat the substrate until the ceramic is formed. The oxide and hydroxide in the alkali silicate are used to lower the softening point of the powdered glass material, and when the powdered glass material is in the semi fluid state, the air and crystal water brought by the alkali silicate will expand and form air bubbles due to the high temperature. In the meantime, the water-soluble polymer at the high temperature will also be converted into a gas which will escape and form close pores inside the finished goods, and pores with open passages on the surface of the finished goods, such that the finished goods will have the lightweight, insulating, refractory, and water-resisting features.

摘要翻译： 本发明公开了一种轻质陶瓷的制造方法，其特征在于，以一定比例混合粉末状玻璃材料，粉状碱性硅酸盐，水溶性聚合物等原料，形成干燥基材，然后加入水 基底成为所需形状; 将基材中的水烘干后，加热基材直到形成陶瓷。 碱性硅酸盐中的氧化物和氢氧化物用于降低玻璃粉末的软化点，当玻璃粉末处于半流体状态时，由碱性硅酸盐引起的空气和结晶水将膨胀并形成气泡 由于高温。 同时，高温下的水溶性聚合物也将转化为成品中的逸出和形成紧密孔的气体，以及在成品表面上具有开放通道的孔，使成品 将具有轻质，绝缘，耐火和防水的特点。