公开(公告)号：US20240345086A1

公开(公告)日：2024-10-17

申请号：US18579635

申请日：2022-07-20

Applicant: UCL Business LTD

Inventor： Kevin Mills ,  Wendy Heywood ,  Ivan Doykov ,  James Moon

IPC: G01N33/569 ,  G01N33/68

CPC classification number: G01N33/56983 ,  G01N33/6848 ,  G01N2333/165 ,  G01N2469/20

Abstract: The present invention relates to a high throughput targeted proteomics-based method for identifying and quantifying one or more binding molecules in a biological fluid of an individual. The present invention involves the use of a plurality of bait molecules to capture the binding molecules. The invention uses proteomics techniques, such as mass spectrometry, for the identification and quantification of the binding molecules. The methods of the invention can be used to identify and quantify antibodies produced by an individual, and proteins expressed by an individual, following exposure to antigens, such as viral antigens, particularly coronavirus antigens and more particularly SARS-CoV-2. Exposure may be via vaccination or following natural infection. The methods of the invention can be used to identify and quantify molecules which bind to a range of proteins of interest, such as autoantigens and neoantigens, as well as to viral vectors.