公开(公告)号：US10023844B2

公开(公告)日：2018-07-17

申请号：US14904364

申请日：2014-05-21

Applicant: UMN PHARMA INC. ,  Timo Vesikari ,  Vesna Blazevic

Inventor： Masahiro Okada ,  Akiko Mukai ,  Tomonori Nishino ,  Daisuke Arinobu ,  Hiroyuki Ito ,  Mamoru Satoh

IPC: C12N7/02 ,  C12N7/00

CPC classification number: C12N7/00 ,  C12N2770/16023 ,  C12N2770/16051

Abstract: The present invention aims at establishing a novel means for purifying norovirus VLPs. There is provided a purification method of norovirus virus-like particles, comprising: contacting solution containing norovirus virus-like particles with support for hydroxyapatite chromatography, binding the virus-like particles to the support, subsequently washing the support with buffer, and then eluting the virus-like particles from the support with buffer containing phosphate, wherein the phosphate concentration of the buffer used for elution is less than 10 mM.