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    NOVEL EXPRESSION VECTORS AND USES THEREOF
    2.
    发明申请
    NOVEL EXPRESSION VECTORS AND USES THEREOF 审中-公开
    新兴表达载体及其用途

    公开(公告)号：US20090252707A1

    公开(公告)日：2009-10-08

    申请号：US12360615

    申请日：2009-01-27

    Applicant: KAI KROHN ,  VESNA BLAZEVIC ,  MARJA TAHTINEN ,  MART USTAV ,  URVE TOOTS ,  ANDRES MANNIK ,  ANNAMARI RANKI ,  ENE USTAV

    Inventor： KAI KROHN ,  VESNA BLAZEVIC ,  MARJA TAHTINEN ,  MART USTAV ,  URVE TOOTS ,  ANDRES MANNIK ,  ANNAMARI RANKI ,  ENE USTAV

    IPC: A61K48/00 ,  A61K35/76 ,  A61K31/713 ,  A61P31/18

    CPC classification number: C07K14/005 ,  A61K48/00 ,  A61K2039/53 ,  C12N15/85 ,  C12N2800/108 ,  C12N2830/42 ,  C12N2840/20 ,  C12N2840/203

    Abstract: A method for treating an HIV disease in a subject in need of said treatment, comprising administering to the subject a therapeutically effective amount of a DNA vaccine comprising an expression vector and a pharmaceutically acceptable excipient, where the expression vector comprises: (a) a heterologous promoter operatively linked to a DNA sequence encoding a nuclear-anchoring protein, where the nuclear-anchoring protein comprises: (i) a DNA binding domain which binds to a specific DNA binding sequence, and (ii) a functional domain of the Bovine Papilloma Virus Type 1 E2 protein, where the functional domain binds to a nuclear component; (b) a multimerized DNA sequence that forms a binding site for the nuclear anchoring protein; and (c) at least one expression cassette comprising a DNA sequence encoding a protein or peptide that stimulates an immune response specific to the protein or peptide; where the expression vector lacks an origin of replication functional in mammalian cells.

    Abstract translation: 一种用于治疗需要所述治疗的受试者中的HIV疾病的方法，包括给予受试者治疗有效量的包含表达载体和药学上可接受的赋形剂的DNA疫苗，其中所述表达载体包含：（a）异源 启动子可操作地连接到编码核锚定蛋白的DNA序列，其中核 - 锚定蛋白包含：（i）结合特异性DNA结合序列的DNA结合结构域，和（ii）牛乳头状瘤病毒的功能结构域 1型E2蛋白，其中功能结构域结合核成分; （b）形成核锚定蛋白的结合位点的多聚化DNA序列; 和（c）至少一个表达盒，其包含编码蛋白质或肽的DNA序列，所述DNA序列刺激对所述蛋白质或肽特异性的免疫应答; 其中表达载体缺乏在哺乳动物细胞中起作用的复制起点。

    Expression vectors and uses thereof
    4.
    发明授权
    Expression vectors and uses thereof 有权
    表达载体及其用途

    公开(公告)号：US07498314B2

    公开(公告)日：2009-03-03

    申请号：US10138098

    申请日：2002-05-03

    Applicant: Kai Krohn ,  Vesna Blazevic ,  Marja Tähtinen ,  Mart Ustav ,  Urve Toots ,  Andres Männik ,  Annamari Ranki ,  Ene Ustav

    Inventor： Kai Krohn ,  Vesna Blazevic ,  Marja Tähtinen ,  Mart Ustav ,  Urve Toots ,  Andres Männik ,  Annamari Ranki ,  Ene Ustav

    IPC: A61K48/00 ,  A61K31/711 ,  C07H21/04 ,  C12N5/10 ,  C12N1/21 ,  C12P21/00

    CPC classification number: C07K14/005 ,  A61K48/00 ,  A61K2039/53 ,  C12N15/85 ,  C12N2800/108 ,  C12N2830/42 ,  C12N2840/20 ,  C12N2840/203

    Abstract: The present invention relates to novel vectors, to DNA vaccines and gene therapeutics containing said vectors, to methods for the preparation of the vectors and DNA vaccines and gene therapeutics, and to therapeutic uses of said vectors. More specifically, the present invention relates to novel vectors comprising an expression cassette of a gene of a nuclear-anchoring protein, which contains a DNA binding domain capable of binding to a specific DNA sequence and a functional domain capable of binding to a nuclear component and a multimerized DNA sequence forming a binding site for the nuclear-anchoring protein, and optionally an expression cassette of a gene, genes or a DNA sequence or DNA sequences of interest. The present invention further relates to DNA vaccines and gene therapeutics containing the novel vectors, to methods for the preparation of the novel vectors and the DNA vaccines and gene therapeutics.

    Abstract translation: 本发明涉及新型载体，DNA疫苗和含有所述载体的基因治疗剂，用于制备载体和DNA疫苗和基因治疗剂的方法以及所述载体的治疗用途。 更具体地，本发明涉及包含核锚定蛋白的基因的表达盒的新载体，其包含能够结合特定DNA序列的DNA结合结构域和能够结合核成分的功能结构域， 形成核 - 锚定蛋白的结合位点的多聚DNA序列，以及任选的基因的表达盒，感兴趣的DNA序列或DNA序列。 本发明还涉及含有新载体的DNA疫苗和基因治疗剂，用于制备新载体和DNA疫苗和基因治疗剂的方法。

    PURIFICATION METHOD FOR VIRUS-LIKE PARTICLES
    7.
    发明申请
    PURIFICATION METHOD FOR VIRUS-LIKE PARTICLES 有权
    用于病毒颗粒的纯化方法

    公开(公告)号：US20160168543A1

    公开(公告)日：2016-06-16

    申请号：US14904364

    申请日：2014-05-21

    Applicant: Timo VESIKARI ,  Vesna BLAZEVIC ,  UMN PHARMA INC.

    Inventor： Masahiro OKADA ,  Akiko MUKAI ,  Tomonori NISHINO ,  Daisuke ARINOBU ,  Hiroyuki TO ,  Mamoru SATOH

    IPC: C12N7/00

    CPC classification number: C12N7/00 ,  C12N2770/16023 ,  C12N2770/16051

    Abstract: The present invention aims at establishing a novel means for purifying norovirus VLPs. There is provided a purification method of norovirus virus-like particles, comprising: contacting solution containing norovirus virus-like particles with support for hydroxyapatite chromatography, binding the virus-like particles to the support, subsequently washing the support with buffer, and then eluting the virus-like particles from the support with buffer containing phosphate, wherein the phosphate concentration of the buffer used for elution is less than 10 mM.

    Abstract translation: 本发明旨在建立一种纯化诺如病毒VLP的新方法。 提供了诺如病毒病毒样颗粒的纯化方法，其包括：将含有诺如病毒病毒样颗粒的溶液与羟基磷灰石层析用载体接触，将病毒样颗粒结合到载体上，随后用缓冲液洗涤载体， 来自含有磷酸盐缓冲液的载体的病毒样颗粒，其中用于洗脱的缓冲液的磷酸盐浓度小于10mM。

    Novel expression vectors and uses thereof
    8.
    发明申请
    Novel expression vectors and uses thereof 有权
    新型表达载体及其用途

    公开(公告)号：US20050026137A1

    公开(公告)日：2005-02-03

    申请号：US10476615

    申请日：2002-05-03

    Applicant: Kai Krohn ,  Vesna Blazevic ,  Marja Tahtinen ,  Mart Ustav ,  Urve Toots ,  Andres Mannjk ,  Annamari Ranki ,  Ene Ustav

    Inventor： Kai Krohn ,  Vesna Blazevic ,  Marja Tahtinen ,  Mart Ustav ,  Urve Toots ,  Andres Mannjk ,  Annamari Ranki ,  Ene Ustav

    IPC: A61K48/00 ,  C12N15/85 ,  C12Q1/70 ,  C12N15/86

    CPC classification number: C07K14/005 ,  A61K48/00 ,  A61K2039/53 ,  C12N15/85 ,  C12N2800/108 ,  C12N2830/42 ,  C12N2840/20 ,  C12N2840/203

    Abstract: The present invention relates to novel vectors, to DNA vaccines and gene therapeutics containing said vectors, to methods for the preparation of the vectors and DNA vaccines and gene therapeutics containing the vectors, and to therapeutic uses of said vectors. More specifically, the present invention relates to novel vectors comprising (a) an expression cassette of a gene of a nuclear-anchoring protein, which contains (i) a DNA binding domain capable of binding to a specific DNA sequence and (ii) a functional domain capable of binding to a nuclear component and (b) a multimerized DNA sequence forming a binding site for the anchoring protein, and optionally (c) one or more expression cassettes of a DNA sequence of interest. In particular the invention relates to vectors that lack a papilloma virus origin of replication. The nuclear-anchoring protein might be the E2 protein of Bovine Papilloma Virus type 1 or Epstein-Barr Virus Nuclear Antigen 1. The invention also relates to vectors that lack an origin of replication functional in a mammalian cell. The invention further relates to methods for expressing a DNA sequence of interest in a subject.

    Abstract translation: 本发明涉及新载体，DNA疫苗和含有所述载体的基因治疗剂，用于制备载体和DNA疫苗的方法以及含有载体的基因治疗剂以及所述载体的治疗用途。 更具体地说，本发明涉及包含（a）核锚定蛋白基因的表达盒的新载体，其包含（i）能够结合特定DNA序列的DNA结合结构域和（ii）功能性 能够结合核组分的结构域和（b）形成锚定蛋白的结合位点的多聚化DNA序列，以及任选（c）感兴趣的DNA序列的一个或多个表达盒。 特别地，本发明涉及缺乏乳头瘤病毒复制起点的载体。 核锚定蛋白可能是牛乳头瘤病毒1型或爱泼斯坦 - 巴尔病毒核抗原1的E2蛋白。本发明还涉及在哺乳动物细胞中缺乏复制起点的载体。 本发明还涉及在受试者中表达感兴趣的DNA序列的方法。

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