公开(公告)号：US12195707B2

公开(公告)日：2025-01-14

申请号：US17960099

申请日：2022-10-04

Applicant: UNITED ARAB EMIRATES UNIVERSITY

Inventor： Saleha Al-Mardeai ,  Sulaiman Al-Zuhair ,  Bart Van Der Bruggen ,  Emad Elnajjar ,  Boguslaw Kruczek

IPC: C12N9/24 ,  C12M1/00 ,  C12M1/12 ,  C12M1/40 ,  C12N9/42 ,  C12P7/10 ,  C13K1/04

Abstract: The membrane bioreactor for simultaneous enzymatic cellulose hydrolysis and product separation is a vessel having a lower hydrolysis reaction chamber and an upper distilled water chamber separated by a semipermeable membrane attached to the top of the lower reaction chamber. The membrane is supported on a stainless steel mesh and sealed to the mesh by epoxy glue to prevent leakage. A peristaltic pump is connected to the reaction chamber and maintains a flow of distilled water through the membrane and the upper chamber, the effluent being collected in a beaker or other product collection vessel. The reaction chamber is agitated at a moderate rate by a magnetic stirrer, and the upper chamber is agitated more rigorously by a mechanical stirrer. A thermocouple and temperature controller and a buffer solution, respectively, maintain temperature and pH in the reaction chamber optimal for enzymatic hydrolysis of cellulose.