公开(公告)号：US20230193320A1

公开(公告)日：2023-06-22

申请号：US17948313

申请日：2022-09-20

Applicant: UNIVERSITÄT BASEL

Inventor： Mara KORNETE ,  Lukas JEKER

IPC: C12N15/90 ,  C12N15/10 ,  A61P35/00 ,  A61K35/17 ,  A61K35/28 ,  C12N5/0783 ,  C12N5/0789

CPC classification number: C12N15/902 ,  C12N15/102 ,  A61P35/00 ,  A61K35/17 ,  A61K35/28 ,  C12N5/0636 ,  C12N5/0647

Abstract: The invention relates to a method to determine a homology directed repair (HDR) event within a eukaryotic cell, wherein the cell expresses a first isoform of a surface protein, which is different from a second isoform of said surface protein with regard to an amino acid marker. The method comprises the steps of inducing a DNA double strand break, providing a HDR template DNA construct comprising the amino acid marker corresponding to the second isoform of the surface protein and subsequently determining the expression of the first or second isoform of said surface protein on said cell, wherein expression of the second isoform indicates a successful HDR event. The invention also relates to a method for editing a genomic location of interest within a eukaryotic cell, and to a method of selectively depleting or enriching an edited cell in a composition of non-edited and edited cells.

公开(公告)号：US20190136263A1

公开(公告)日：2019-05-09

申请号：US16096074

申请日：2017-04-25

Applicant: UNIVERSITÄT BASEL

Inventor： Mara KORNETE ,  Lukas JEKER

IPC: C12N15/90 ,  A61K35/28 ,  A61K35/17 ,  A61P35/00 ,  C12N5/0789 ,  C12N5/0783

Abstract: The invention relates to a method to determine a homology directed repair (HDR) event within a eukaryotic cell, wherein the cell expresses a first isoform of a surface protein, which is different from a second isoform of said surface protein with regard to an amino acid marker. The method comprises the steps of inducing a DNA double strand break, providing a HDR template DNA construct comprising the amino acid marker corresponding to the second isoform of the surface protein and subsequently determining the expression of the first or second isoform of said surface protein on said cell, wherein expression of the second isoform indicates a successful HDR event. The invention also relates to a method for editing a genomic location of interest within a eukaryotic cell, and to a method of selectively depleting or enriching an edited cell in a composition of non-edited and edited cells.