公开(公告)号：US20150267256A1

公开(公告)日：2015-09-24

申请号：US14438074

申请日：2013-10-30

Applicant: UNIVERSITÄTSSPITAL BASEL

Inventor： Jochen Kinter ,  Michael Sinnreich

IPC: C12Q1/68

CPC classification number: C12Q1/6883 ,  C12Q1/6858 ,  C12Q1/6888 ,  C12Q2600/16 ,  C12Q2525/204 ,  C12Q2537/143 ,  C12Q2549/119

Abstract: The present invention relates to a method for the simultaneous amplification of a plurality of different nucleic acid target sequences comprising the steps of providing a plurality of different nucleic acid polymers as templates, each template comprising a specific target sequence and a primer annealing sequence located downstream of the target sequence, and amplifying the template by a polymerase dependent amplification reaction using a primer oligonucleotide comprising a primer sequence which is at least essentially complementary to the primer annealing sequence. The method is characterized in that for the polymerase dependent amplification reaction a set of primer oligonucleotides is used, said set comprising at least two primer oligonucleotides which are able to anneal to the primer annealing sequence of the same template and which differ from each other in the efficiency for the polymerase dependent amplification reaction to take place.

Abstract translation: 本发明涉及同时扩增多个不同核酸靶序列的方法，包括以下步骤：提供多种不同的核酸聚合物作为模板，每个模板包含特定的靶序列和位于 靶序列，并使用包含与引物退火序列至少基本上互补的引物序列的引物寡核苷酸，通过聚合酶依赖性扩增反应扩增模板。 该方法的特征在于，对于聚合酶依赖性扩增反应，使用一组引物寡核苷酸，所述组包含至少两个引物寡核苷酸，其能够退火到相同模板的引物退火序列，并且在 聚合酶依赖性扩增反应发生的效率。