公开(公告)号：US20160137975A1

公开(公告)日：2016-05-19

申请号：US14904396

申请日：2014-07-10

申请人： UNIVERSITY OF PITTSBURGH - OF THE COMMONWEALTH SYSTEM OF HIGHER EDUCATION

发明人： Cyril Yeshwant Ramathal ,  Jens Dumuthy-Durruthy ,  Renee A. Reijo Pera ,  Kyle E. Orwig

IPC分类号： C12N5/076 ,  A61K9/00 ,  A61K35/52 ,  C12N5/074 ,  A61K35/545

CPC分类号： C12N5/061 ,  A61K9/0034 ,  A61K35/52 ,  A61K35/545 ,  C12N5/0696 ,  C12N2501/602 ,  C12N2501/603 ,  C12N2501/604 ,  C12N2501/605 ,  C12N2501/606 ,  C12N2501/608 ,  C12N2506/02 ,  C12N2506/1307 ,  C12N2506/45 ,  C12N2510/00

摘要： Methods are provided for the generation of male germ cells from somatic cells. Included are methods of non-integrative reprogramming for germ cell differentiation with a reduced risk of neoplasia during in vivo differentiation by the inclusion of VASA with the reprogramming factors. Also included are methods of generating male germ cells from reprogrammed pluripotent cells by direct injection of the reprogrammed cells into the seminiferous tubules. In some embodiments the somatic cells are derived from a male with oligospermia or azoospermia, which may be the result of a genetic abnormality in Azoospermia Factor (AZF) region.

摘要翻译： 提供用于从体细胞产生雄性生殖细胞的方法。 包括生物细胞分化的非综合重编程方法，通过包含VASA与重编程因子在体内分化过程中瘤形成风险降低。 还包括通过将重编程细胞直接注射到生精小管中从重编程的多能细胞产生雄性生殖细胞的方法。 在一些实施方案中，体细胞衍生自具有精子不足或无精子症的雄性，这可能是无精症因子（AZF）区域遗传异常的结果。