公开(公告)号：US11293028B2

公开(公告)日：2022-04-05

申请号：US15765132

申请日：2016-10-03

申请人： University of Utah Research Foundation

发明人： Kelly T. Hughes ,  Fabienne Chevance

IPC分类号： C12N15/00 ,  C12N15/74 ,  C07K14/62 ,  C07K14/245

摘要： The present invention relates to the effects of codon context and synonymous codon changes on mRNA translation and methods of increasing protein production.

公开(公告)号：US10800818B2

公开(公告)日：2020-10-13

申请号：US15459191

申请日：2017-03-15

申请人： University of Utah Research Foundation

发明人： Kelly T. Hughes ,  Baldomero M. Olivera

IPC分类号： C07K14/245 ,  C07K14/255 ,  C07K14/34 ,  C07K14/435 ,  C07K14/46 ,  C12P21/02 ,  C12N15/62 ,  C12N15/70 ,  C12N15/74

摘要： Disclosed are compositions and methods for expressing and purifying a peptide of interest using a Flagellar Type III secretion system. Disclosed are nucleic acid sequences that contain a FlgM nucleic acid sequence, a cleavage site, and a nucleic acid sequence of interest. Also disclosed are polypeptides that contain FlgM, a cleavage site and a peptide of interest. Methods of producing polypeptides that have FlgM, a cleavage site and a peptide of interest are provided.

公开(公告)号：US09630997B2

公开(公告)日：2017-04-25

申请号：US14404919

申请日：2013-05-30

申请人： UNIVERSITY OF UTAH RESEARCH FOUNDATION

发明人： Kelly T. Hughes ,  Baldomero M. Olivera

IPC分类号： C07K14/245 ,  C12N15/74 ,  C07K14/255 ,  C07K14/34 ,  C07K14/435 ,  C07K14/46 ,  C12P21/02 ,  C12N15/62 ,  C12N15/70

CPC分类号： C07K14/245 ,  C07K14/255 ,  C07K14/34 ,  C07K14/43504 ,  C07K14/43518 ,  C07K14/43522 ,  C07K14/46 ,  C07K2319/036 ,  C07K2319/21 ,  C07K2319/50 ,  C12N15/62 ,  C12N15/70 ,  C12N15/74 ,  C12P21/02

摘要： Disclosed are compositions and methods for expressing and purifying a peptide of interest using a Flagellar Type III secretion system. Disclosed are nucleic acid sequences that contain a FlgM nucleic acid sequence, a cleavage site, and a nucleic acid sequence of interest. Also disclosed are polypeptides that contain FlgM, a cleavage site and a peptide of interest. Methods of producing polypeptides that have FlgM, a cleavage site and a peptide of interest are provided.

公开(公告)号：US20150225466A1

公开(公告)日：2015-08-13

申请号：US14404919

申请日：2013-05-30

申请人： UNIVERSITY OF UTAH RESEARCH FOUNDATION

发明人： Kelly T. Hughes ,  Baldomero M. Olivera

IPC分类号： C07K14/245 ,  C12P21/02 ,  C07K14/255 ,  C07K14/46 ,  C07K14/34 ,  C12N15/74 ,  C07K14/435

CPC分类号： C07K14/245 ,  C07K14/255 ,  C07K14/34 ,  C07K14/43504 ,  C07K14/43518 ,  C07K14/43522 ,  C07K14/46 ,  C07K2319/036 ,  C07K2319/21 ,  C07K2319/50 ,  C12N15/62 ,  C12N15/70 ,  C12N15/74 ,  C12P21/02

摘要： Disclosed are compositions and methods for expressing and purifying a peptide of interest using a Flagellar Type III secretion system. Disclosed are nucleic acid sequences that contain a FlgM nucleic acid sequence, a cleavage site, and a nucleic acid sequence of interest. Also disclosed are polypeptides that contain FlgM, a cleavage site and a peptide of interest. Methods of producing polypeptides that have FlgM, a cleavage site and a peptide of interest are provided.

摘要翻译： 公开了使用鞭毛III型分泌系统表达和纯化目标肽的组合物和方法。 公开了含有FlgM核酸序列，切割位点和感兴趣的核酸序列的核酸序列。 还公开了含有FlgM，切割位点和目的肽的多肽。 提供了产生具有FlgM，切割位点和目的肽的多肽的方法。