公开(公告)号：US11028383B2

公开(公告)日：2021-06-08

申请号：US16676253

申请日：2019-11-06

Applicant: UNIVERSITY OF WASHINGTON ,  UNIVERSITY OF UTAH

Inventor： Neil King ,  Wesley Sundquist ,  Joerg Votteler ,  Yang Hsia ,  David Baker ,  Jacob Bale ,  Marc Lajoie ,  Gabriel Butterfield ,  Elizabeth Gray ,  Daniel Stetson

IPC: C12N9/88 ,  C07K14/00 ,  C07K14/435

Abstract: The application discloses multimeric assemblies including multiple oligomeric substructures, where each oligomeric substructure includes multiple proteins that self-interact around at least one axis of rotational symmetry, where each protein includes one or more polypeptide-polypeptide interface (“O interface”); and one or more polypeptide domain that is capable of effecting membrane scission and release of an enveloped multimeric assembly from a cell by recruiting the ESCRT machinery to the site of budding by binding to one or more proteins in the eukaryotic ESCRT complex (“L domain”); and where the multimeric assembly includes one or more subunits comprising one or more polypeptide domain that is capable of interacting with a lipid bilayer (“M domain”), as well as membrane-enveloped versions of the multimeric assemblies.

公开(公告)号：US20230313167A1

公开(公告)日：2023-10-05

申请号：US18174041

申请日：2023-02-24

Applicant: University of Washington ,  University of Utah Research Foundation

Inventor： Neil King ,  Wesley Sundquist ,  Joerg Votteler ,  Yang Hsia ,  David Baker ,  Jacob Bale ,  Marc Lajoie ,  Gabriel Butterfield ,  Elizabeth Gray ,  Daniel Stetson

IPC: C12N9/88 ,  C07K14/00 ,  C07K14/435

CPC classification number: C12N9/88 ,  C07K14/00 ,  C07K14/435 ,  C12Y401/02014 ,  C07K2319/03 ,  C07K2319/06 ,  C07K2319/735

Abstract: The application discloses multimeric assemblies including multiple oligomeric substructures, where each oligomeric substructure includes multiple proteins that self-interact around at least one axis of rotational symmetry, where each protein includes one or more polypeptide-polypeptide interface (“O interface”); and one or more polypeptide domain that is capable of effecting membrane scission and release of an enveloped multimeric assembly from a cell by recruiting the ESCRT machinery to the site of budding by binding to one or more proteins in the eukaryotic ESCRT complex (“L domain”); and where the multimeric assembly includes one or more subunits comprising one or more polypeptide domain that is capable of interacting with a lipid bilayer (“M domain”), as well as membrane-enveloped versions of the multimeric assemblies.