公开(公告)号：US20240226269A9

公开(公告)日：2024-07-11

申请号：US18278208

申请日：2022-02-18

申请人： UNIVERZITA PALACKEHO V OLOMOUCI ,  NEXIUM, S.R.O.

发明人： Petr MALY ,  Milan RASKA ,  Milan KUCHAR ,  Petr KOSZTYU ,  Jiri CERNY ,  Veronika DANIEL LISKOVA ,  Hana PETROKOVA

IPC分类号： A61K39/21 ,  A61P31/18 ,  C07K14/005

CPC分类号： A61K39/21 ,  A61P31/18 ,  C07K14/005 ,  A61K2039/575

摘要： The present invention provides polypeptides having a length of up to 180 amino acids and containing a sequence selected from sequences identical or differing at most in 5 amino acids from the sequence: KSELAVEILEKGQVRFWMQAX21X22X23X24GNAKVNYIFNEKEIFEGPKYKMHIDXsoXsiXszGIIEM FMEKLQDEDEGTYTFQLQX76X77X78X79X80NHSTVVLVGDVFKKLQKEAEFQRQEWIRKQG (SEQ ID NO. 2), wherein X21X22X23X24 is selected from KAQQ (SEQ ID NO. 33), LSVF (SEQ ID NO. 34), ATPS (SEQ ID NO. 35), EIMW (SEQ ID NO. 36), DGSS (SEQ ID NO. 37), LLPL (SEQ ID NO. 38), WMWW (SEQ ID NO. 39), MNLY (SEQ ID NO. 40), MWRN (SEQ ID NO. 41), IMME (SEQ ID NO. 42), KHQL (SEQ ID NO. 43), HWQF (SEQ ID NO. 44), YAGN (SEQ ID NO. 45) and HGQW (SEQ ID NO. 46); X50X51X52 is selected from RNT, IMF, GHE, PSW, RAN, YFW, ITL, QAM, DMR, WLW, QGE, VQY and VSL; X76X77X78X79X80 is selected from SHHLG (SEQ ID NO. 47), FMLMM (SEQ ID NO. 48), VILIL (SEQ ID NO. 49), IVTPL (SEQ ID NO. 50), DFIIW (SEQ ID NO. 51), MWSE (deletion) (SEQ ID NO. 52), LYYAW (SEQ ID NO. 53), MMIEY (SEQ ID NO. 54), WMTQT (SEQ ID NO. 55), PQLWL (SEQ ID NO. 56), EPIFL (SEQ ID NO. 57) and QTATY (SEQ ID NO. 58); and optionally further having an affinity tag attached to the N-terminus or to the C-terminus of the sequence. These polypeptides mimic HIV-1 Env glycoprotein epitopes and are useful in preparation of antibodies and vaccines.

公开(公告)号：US20240131147A1

公开(公告)日：2024-04-25

申请号：US18278208

申请日：2022-02-18

申请人： UNIVERZITA PALACKEHO V OLOMOUCI ,  NEXIUM, S.R.O.

发明人： Petr MALY ,  Milan RASKA ,  Milan KUCHAR ,  Petr KOSZTYU ,  Jiri CERNY ,  Veronika DANIEL LISKOVA ,  Hana PETROKOVA

IPC分类号： A61K39/21 ,  A61P31/18 ,  C07K14/005

CPC分类号： A61K39/21 ,  A61P31/18 ,  C07K14/005 ,  A61K2039/575

摘要： The present invention provides polypeptides having a length of up to 180 amino acids and containing a sequence selected from sequences identical or differing at most in 5 amino acids from the sequence: KSELAVEILEKGQVRFWMQAX21X22X23X24GNAKVNYIFNEKEIFEGPKYKMHIDXsoXsiXszGIIEM FMEKLQDEDEGTYTFQLQX76X77X78X79X80NHSTVVLVGDVFKKLQKEAEFQRQEWIRKQG (SEQ ID NO. 2), wherein X21X22X23X24 is selected from KAQQ (SEQ ID NO. 33), LSVF (SEQ ID NO. 34), ATPS (SEQ ID NO. 35), EIMW (SEQ ID NO. 36), DGSS (SEQ ID NO. 37), LLPL (SEQ ID NO. 38), WMWW (SEQ ID NO. 39), MNLY (SEQ ID NO. 40), MWRN (SEQ ID NO. 41), IMME (SEQ ID NO. 42), KHQL (SEQ ID NO. 43), HWQF (SEQ ID NO. 44), YAGN (SEQ ID NO. 45) and HGQW (SEQ ID NO. 46); X50X51X52 is selected from RNT, IMF, GHE, PSW, RAN, YFW, ITL, QAM, DMR, WLW, QGE, VQY and VSL; X76X77X78X79X80 is selected from SHHLG (SEQ ID NO. 47), FMLMM (SEQ ID NO. 48), VILIL (SEQ ID NO. 49), IVTPL (SEQ ID NO. 50), DFIIW (SEQ ID NO. 51), MWSE (deletion) (SEQ ID NO. 52), LYYAW (SEQ ID NO. 53), MMIEY (SEQ ID NO. 54), WMTQT (SEQ ID NO. 55), PQLWL (SEQ ID NO. 56), EPIFL (SEQ ID NO. 57) and QTATY (SEQ ID NO. 58); and optionally further having an affinity tag attached to the N-terminus or to the C-terminus of the sequence. These polypeptides mimic HIV-1 Env glycoprotein epitopes and are useful in preparation of antibodies and vaccines.

公开(公告)号：US20220402978A1

公开(公告)日：2022-12-22

申请号：US17642131

申请日：2020-09-08

申请人： UNIVERZITA PALACKEHO V OLOMOUCI

发明人： Petr MALY ,  Milan RASKA ,  Milan KUCHAR ,  Petr KOSZTYU ,  Jaroslav TURANEK ,  Hana PETROKOVA

IPC分类号： C07K14/16 ,  A61P31/18 ,  A61K39/21 ,  C12N15/113

摘要： A polypeptide mimicking epitope of glycoprotein gp120 of HIV-1 virus that is recognized by a paratope of broadly neutralizing antibody VRC01 and has the length up to 100 amino acid residues and contains an amino acid sequence: (SEQ ID NO. 1): X1YKNX2INX3AX4X5VX6X7VKRX8IDX9ILAX10LP X1 is selected from amino acids A, N, R; X2 is selected from amino acids A, R, D; X3 is selected from amino acids R, V, P; X4 is selected from amino acids V, L, S; X5 is selected from amino acids T, G, R; X6 is selected from amino acids G, T; X7 is selected from amino acids L, A; X8 is selected from amino acids V, I; X9 is selected from amino acids G, A, R; X10 is selected from amino acids R, A, G; with a directly attached alpha-helical structure at the N-terminus or C-terminus is disclosed.