公开(公告)号：US20240081302A1

公开(公告)日：2024-03-14

申请号：US18486336

申请日：2023-10-13

申请人： UNOGEN BIOTECHNOLOGY CO., LTD.

发明人： Tao CHEN ,  Bin YUE ,  Shuang LV ,  Wei ZHANG

IPC分类号： A01K67/027 ,  C07K16/06 ,  C12N9/22 ,  C12N15/85 ,  C12N15/90

CPC分类号： A01K67/0278 ,  C07K16/06 ,  C12N9/22 ,  C12N15/8509 ,  C12N15/907 ,  A01K2217/072 ,  A01K2217/15 ,  A01K2227/105 ,  A01K2267/01

摘要： A method of preparing an antibody carrying a universal site-directed coupling interface based on a genetically modified vertebrate is described. The method of constructs a vertebrate model, the vertebrate model is used to produce an antibody carrying a universal site-directed coupling interface. The method includes site-directly knocking in a coding gene of a specific recognition sequence of a certain ligase A or a coding gene of a certain intein A at a certain position A of a certain coding gene A of the immunoglobulin in the genome of the recipient animal, to obtain the vertebrate model. The modified mice can normally stimulate immune responses, produce a polyclonal antibody and a monoclonal antibody with a site-directed linking site. Antibodies with a site-directed linking site can normally carry out site-directed linking, and add various application groups to realize downstream applications of antibodies, such as ELISA, immunofluorescence staining, and immuno-PCR.