公开(公告)号：US09683982B2

公开(公告)日：2017-06-20

申请号：US14678156

申请日：2015-04-03

Applicant: Universität Leipzig

Inventor： Josef Käs ,  Jochen Guck

IPC: G01N33/483 ,  G01N33/50 ,  G01L1/24

CPC classification number: G01N33/4833 ,  G01L1/24 ,  G01L1/241 ,  G01N33/5011

Abstract: In a method for diagnosing and/or prognosis of cancers, diagnosing origin of tumor cells, optimizing cancer therapy, and screening active substances for oncology, the mechanical properties of tumor cells and reference cells are analyzed under mechanical load that causes linear or non-linear deformation of the loaded cell. The engineering strain of the cells caused by directed mechanical stress being introduced is used to determine metastasis risk and the presence of uncontrollably proliferating and/or invasive cells, or the origin of the tumor. The metastasis risk is determined based on the proportion of cells in the sample exhibiting engineering strain in a direction opposite to the stressing direction. The risk of the presence of uncontrollably proliferating cells for non-linear deformation of the cell is determined in the sample based on the mean value of the engineering strain in the direction of cell stressing.

公开(公告)号：US20150276706A1

公开(公告)日：2015-10-01

申请号：US14678156

申请日：2015-04-03

Applicant: Universität Leipzig

Inventor： Josef Käs ,  Jochen Guck

IPC: G01N33/483 ,  G01N33/50

CPC classification number: G01N33/4833 ,  G01L1/24 ,  G01L1/241 ,  G01N33/5011

Abstract: In a method for diagnosing and/or prognosis of cancers, diagnosing origin of tumor cells, optimizing cancer therapy, and screening active substances for oncology, the mechanical properties of tumor cells and reference cells are analyzed under mechanical load that causes linear or non-linear deformation of the loaded cell. The engineering strain of the cells caused by directed mechanical stress being introduced is used to determine metastasis risk and the presence of uncontrollably proliferating and/or invasive cells, or the origin of the tumor. The metastasis risk is determined based on the proportion of cells in the sample exhibiting engineering strain in a direction opposite to the stressing direction. The risk of the presence of uncontrollably proliferating cells for non-linear deformation of the cell is determined in the sample based on the mean value of the engineering strain in the direction of cell stressing.

Abstract translation: 在癌症诊断和/或预后的方法中，诊断肿瘤细胞的起源，优化癌症治疗和筛选肿瘤学活性物质，在机械载荷下分析肿瘤细胞和参照细胞的机械性质，其导致线性或非线性 加载单元的变形。 由导向的机械应力引起的细胞的工程菌株用于确定转移风险和不可控制的增殖和/或侵入性细胞的存在或肿瘤的起源。 转移风险基于在与应激方向相反的方向显示工程应变的样品中的细胞比例来确定。 基于工程应变在细胞应激方向上的平均值，在样品中确定存在不可控制的增殖细胞用于细胞非线性变形的风险。