METHOD OF INDUCING BETA CELLS FROM URINE-DERIVED CELLS USING SMALL MOLECULES

    公开(公告)号:US20180016556A1

    公开(公告)日:2018-01-18

    申请号:US15538465

    申请日:2015-12-22

    IPC分类号: C12N5/071 C12N5/074

    摘要: The disclosure relates to a method of producing induced beta cells from urine-derived cells, the method comprising providing urine-derived cells; inducing the urine-derived cells by culturing said urine-derived cells in a primary induction culture medium comprising an effective amount of at least one small molecule reprogramming factor(s) for a first period of time to obtain induced endoderm cells; inducing the induced endoderm cells by culturing said induced endoderm cells in a secondary induction culture medium comprising an effective amount of at least one small molecule reprogramming factor(s) for a second period of time to obtain induced pancreatic precursor cells; and inducing the induced pancreatic precursor cells by culturing said pancreatic precursor cells in a tertiary induction culture medium comprising an effective amount of at least one small molecule reprogramming factor(s) for a third period of time to obtain induced beta cells.