公开(公告)号：US11820970B2

公开(公告)日：2023-11-21

申请号：US17480078

申请日：2021-09-20

Applicant: University of Washington

Inventor： David Baker ,  David Younger ,  Eric Klavins

IPC: C12N15/81 ,  C12N15/10

CPC classification number: C12N15/1055

Abstract: The present invention relates to methods and compositions for the high throughput screening of protein-protein interactions in yeast liquid culture. Protein fusions non-native to yeast may be expressed to replace endogenous sexual agglutination proteins and mediate library-by-library interrogation of protein interactions. The methods and compositions of the invention can be utilized for the characterization of protein interaction networks in high throughput for both binding affinity and specificity, which is crucial for understanding cellular functions, screening therapeutic candidates, and evaluating engineered protein networks.

公开(公告)号：US11136573B2

公开(公告)日：2021-10-05

申请号：US17091847

申请日：2020-11-06

Applicant: University of Washington

Inventor： David Baker ,  David Younger ,  Eric Klavins

IPC: C12N15/81 ,  C12N15/10

Abstract: The present invention relates to methods and compositions for the high throughput screening of protein-protein interactions in yeast liquid culture. Protein fusions non-native to yeast may be expressed to replace endogenous sexual agglutination proteins and mediate library-by-library interrogation of protein interactions. The methods and compositions of the invention can be utilized for the characterization of protein interaction networks in high throughput for both binding affinity and specificity, which is crucial for understanding cellular functions, screening therapeutic candidates, and evaluating engineered protein networks.

公开(公告)号：US10988759B2

公开(公告)日：2021-04-27

申请号：US16856506

申请日：2020-04-23

Applicant: University of Washington

Inventor： David Baker ,  David Younger ,  Eric Klavins

IPC: C12N15/10

Abstract: The present invention relates to methods and compositions for the high throughput screening of protein-protein interactions in yeast liquid culture. Protein fusions non-native to yeast may be expressed to replace endogenous sexual agglutination proteins and mediate library-by-library interrogation of protein interactions. The methods and compositions of the invention can be utilized for the characterization of protein interaction networks in high throughput for both binding affinity and specificity, which is crucial for understanding cellular functions, screening therapeutic candidates, and evaluating engineered protein networks.

公开(公告)号：US20170369892A1

公开(公告)日：2017-12-28

申请号：US15630817

申请日：2017-06-22

Applicant: University of Washington

Inventor： Eric Klavins ,  Arjun Khakhar ,  Jennifer L. Nemhauser

IPC: C12N15/82 ,  C12N15/01 ,  C12N15/10 ,  C12N15/63 ,  C12N9/22 ,  C12N15/00

CPC classification number: C12N15/82 ,  C12N9/22 ,  C12N15/00 ,  C12N15/01 ,  C12N15/102 ,  C12N15/63 ,  C12N15/8213 ,  C12N15/8217 ,  C12N15/8238 ,  C12N15/8239 ,  C12N15/8286 ,  Y02A40/162

Abstract: Synthetic signal transduction systems are provided. The synthetic signal transduction system may be a hormone degradable CRISPR-based transcription factor including a nuclease null Cas9 protein, a nuclear localization signal, a phytohormone degron, and a transcriptional regulation domain. Methods of generating non-naturally occurring plants are also provided. The methods may include expressing a synthetic signal transduction system in a plant. Non-naturally occurring plants formed by the methods are also provided.