公开(公告)号：US20250034413A1

公开(公告)日：2025-01-30

申请号：US18716703

申请日：2022-12-06

Applicant: VIRGINIA COMMONWEALTH UNIVERSITY

Inventor： Lucas C. OLSON ,  Michael J. MCCLURE ,  Zvi SCHWARTZ ,  Tri M. NGUYEN ,  Barbara D. BOYAN

IPC: C09D11/02 ,  C12N5/077 ,  C12N9/64

Abstract: A method of decellularizing in an animal tissue includes digesting the animal tissue; treating the animal tissue with a surfactant: treating the animal tissue with at least one zwitterionic detergent to form a decellularized animal tissue; and treating the decellularized animal tissue with at least one advanced glycation end-product (AGE) inhibitor to reduce AGE crosslinking in the decellularized animal tissue. The decellularized animal tissue may be ground into a powder and dissolved in a digest solution to form a bioink composition useful for 3D printing an in vitro model of healthy or diseased tissue.