公开(公告)号：US20250109226A1

公开(公告)日：2025-04-03

申请号：US18581758

申请日：2024-02-20

Applicant: VIRGINIA COMMONWEALTH UNIVERSITY

Inventor： Youzhong GUO ,  Weihua AIU ,  Thi Kim Hoang TRINH

IPC: C08F228/02 ,  C07K1/14 ,  C07K17/08

Abstract: Provided is a functionalized polymer useful for extracting, isolating, and/or purifying membrane proteins with their native protein structures being retained. Also provided are a Native Cell Membrane Nanoparticle (NCMN) system which contains the above functionalized polymer and a membrane protein and a method to characterize and/assess the native structure of the membrane protein.

公开(公告)号：US20250026892A1

公开(公告)日：2025-01-23

申请号：US18581769

申请日：2024-02-20

Applicant: VIRGINIA COMMONWEALTH UNIVERSITY

Inventor： Youzhong GUO ,  Weihua QIU ,  Thi Kim Hoang TRINH

IPC: C08G81/02 ,  G01N33/68

Abstract: Provided is a functionalized polymer useful for extracting, isolating, and/or purifying membrane proteins with their native protein structures being retained. Also provided are a Native Cell Membrane Nanoparticle (NCMN) system which contains the above functionalized polymer and a membrane protein and a method to characterize and/assess the native structure of the membrane protein.

公开(公告)号：US20230295349A1

公开(公告)日：2023-09-21

申请号：US18001413

申请日：2021-06-02

Applicant: Virginia Commonwealth University

Inventor： Youzhong GUO

IPC: C07K17/08 ,  C07K1/14 ,  C07K1/36

CPC classification number: C07K17/08 ,  C07K1/14 ,  C07K1/36

Abstract: Provided are a Native Cell Membrane Nanoparticle (NCMN) system and methods of using the NCMN polymers to extract and/or purify one or more membrane proteins. The NCMN system includes at least one NCMN polymer and one or more membrane proteins in their native lipid bilayer membrane. A method of using the NCMN system to extract or isolate the one or more membrane proteins in the form of NCMN system without the use of a detergent while maintaining the protein’s native structure and functional activity is provided.