公开(公告)号：US20240326012A1

公开(公告)日：2024-10-03

申请号：US18623906

申请日：2024-04-01

Applicant: Pierce Biotechnology, Inc.

Inventor： Ramesh Ganapathy ,  Christopher Etienne ,  Deven Etnyre ,  Ashok Salunkhe ,  Atul Deshpande

IPC: B01J20/24 ,  B01D15/34 ,  B01J20/30 ,  C07K1/14

CPC classification number: B01J20/24 ,  B01D15/34 ,  B01J20/3042 ,  C07K1/14

Abstract: The present specification relates to compositions, devices, apparatus, methods, kits and systems for sample preparation (e.g., separation, reduction or removal of small molecules from biomolecules in a sample). Exemplary small molecules that can be separated, reduced or removed have a molecular weight range of

公开(公告)号：US12071459B2

公开(公告)日：2024-08-27

申请号：US17717120

申请日：2022-04-10

Applicant: ZHEJIANG RISING BIOTECHNOLOGY CO., LTD.

Inventor： Jianxin Cao

IPC: C12N15/63 ,  C07K1/18 ,  C07K14/51 ,  C12N15/09 ,  C12N15/66 ,  C07K1/14 ,  C07K1/16

CPC classification number: C07K14/51 ,  C07K1/18 ,  C07K1/14 ,  C07K1/16 ,  C12N15/09 ,  C12N15/63 ,  C12N15/66

Abstract: The present invention relates to a field of biotechnology. It discloses a novel amino acid sequence of recombinant human bone morphogenetic protein-2 (rhBMP-2) and encoded nucleotide sequences thereof, and a method for preparing rhBMP-2. In the present invention, by further optimizing the nucleotide sequence of rhBMP-2, novel amino acid sequence of rhBMP-2 that can express good ectopic induced osteogenic activity and has good renaturation and purification effect is screened out. The engineered bacteria constructed by the present invention can induce the production of recombinant rhBMP-2 protein with an expression level of about 55%, and the produced target protein is more easily denatured and purified than the existing recombinant rhBMP-2, with better denaturation and purification effect and higher osteoinductive activity.

公开(公告)号：US20240279702A1

公开(公告)日：2024-08-22

申请号：US18569001

申请日：2022-06-29

Applicant: CORTEVA AGRISCIENCE LLC ,  FRAUNHOFER-GESELLSCHAFT ZUR FÖRDERUNG DER ANGEWANDTEN FORSCHUNG EV

Inventor： MATTHIAS BUNTRU ,  KRISHNA MADDURI ,  STEFAN SCHILLBERG ,  SIMON VOGEL

IPC: C12P21/00 ,  C07K1/14 ,  C12N5/04 ,  C12Q1/6895

CPC classification number: C12P21/00 ,  C07K1/14 ,  C12N5/04 ,  C12Q1/6895 ,  C12Q2600/13

Abstract: This disclosure concerns the systems, methods, and kits for the in vitro synthesis of biological macromolecules in a reaction utilizing maize or soybean cell lysates.

公开(公告)号：US12059684B2

公开(公告)日：2024-08-13

申请号：US17250217

申请日：2019-06-26

Applicant: The Emerther Company

Inventor： Yongmei Li ,  Li Li ,  Lianhao Zhao

IPC: C07K1/14 ,  B01L3/00

CPC classification number: B01L3/50855 ,  C07K1/14 ,  B01L2200/0668 ,  B01L2300/0609 ,  B01L2300/0829 ,  B01L2400/043

Abstract: A concentrating-enriching magnetic bead purifier includes a base, at least one first mixing sleeve socket, at least one second mixing sleeve socket, and a plurality of wells, and a controlling module. The first mixing sleeve socket includes a first magnetic rod, and a first magnetic rod sleeve. The second mixing sleeve socket includes a second magnetic rod and a second magnetic rod sleeve. A diameter of a cross section of the second magnetic rod sleeve is smaller than that of the first magnetic rod sleeve. The wells include at least one binding well, at least one washing well, and at least one elution well. The volume of the elution well is smaller than that of the binding well. The controlling module controls the first magnetic rod sleeve to only mix or adsorb in the binding well and the washing well and controls the second magnetic rod sleeve to mix or adsorb in the washing well and the elution well.

公开(公告)号：US20240239837A1

公开(公告)日：2024-07-18

申请号：US18155457

申请日：2023-01-17

Applicant: NAJRAN UNIVERSITY

Inventor： Mabkhoot Abdullah ALSAIARI ,  Nawshad MUHAMMAD ,  Sandleen FEROZ ,  George DIAS ,  Saeed A. ALSAREII ,  Farid A. HARRAZ

IPC: C07K1/14 ,  C07K14/47 ,  D01C3/00

CPC classification number: C07K1/14 ,  C07K14/4741 ,  D01C3/00

Abstract: A method for extracting keratin from wool fibers is described. The method includes treating the wool fibers in a solvent, washing, and drying to form cleaned wool fibers. The method incudes cutting the cleaned wool fibers and dispersing in an ionic liquid to form a slurry. The method further includes sonicating the slurry to form a first composition containing dissolved keratin. The method also includes mixing the first composition with water to precipitate the keratin from the first composition. Additionally, the method involves separating and collecting the precipitated keratin from the first composition, washing, and drying to form the keratin. The method for extracting keratin from natural fibers is also described.

公开(公告)号：US20240158527A1

公开(公告)日：2024-05-16

申请号：US18410949

申请日：2024-01-11

Applicant: Genentech, Inc.

Inventor： Yung-Hsiang KAO ,  Michael W. LAIRD ,  Melody Trexler SCHMIDT ,  Rita L. WONG ,  Daniel P. HEWITT

IPC: C07K16/28 ,  A61K39/395 ,  C07K1/14

CPC classification number: C07K16/2887 ,  A61K39/39591 ,  C07K1/14 ,  C07K2317/24

Abstract: The invention concerns methods and means for preventing the reduction of disulfide bonds during the recombinant production of disulfide-containing polypeptides. In particular, the invention concerns the prevention of disulfide bond reduction during harvesting of disulfide-containing polypeptides, including antibodies, from recombinant host cell cultures.

公开(公告)号：US11965851B2

公开(公告)日：2024-04-23

申请号：US17075793

申请日：2020-10-21

Applicant: Purdue Research Foundation

Inventor： David H. Thompson ,  Christopher Benjamin ,  Kyle Wright ,  Scott C. Bolton ,  Seok-Hee Hyun

IPC: G01N27/12 ,  C01B32/192 ,  G01N1/30 ,  G01N27/414 ,  H01J37/20 ,  B82Y30/00 ,  B82Y40/00 ,  C07K1/14

CPC classification number: G01N27/127 ,  C01B32/192 ,  G01N1/30 ,  G01N27/4145 ,  H01J37/20 ,  B82Y30/00 ,  B82Y40/00 ,  C07K1/14 ,  G01N2223/418

Abstract: Methods of using grids in connection with suitable microscopy techniques, such as for determining the structure of target compounds including proteins, are disclosed. Said grid comprising a coating modified with one or more capture agents and a deactivating agent.

公开(公告)号：US11904082B2

公开(公告)日：2024-02-20

申请号：US16322686

申请日：2017-07-31

Applicant: KANEKA CORPORATION ,  JICHI MEDICAL UNIVERSITY

Inventor： Ai Yonezawa ,  Kana Watanabe ,  Makoto Kuroo

IPC: B01D15/00 ,  C07K1/14 ,  A61M1/34 ,  A61M1/36 ,  B01J20/24 ,  B01J20/32

CPC classification number: A61M1/3496 ,  A61M1/3687 ,  B01D15/00 ,  B01J20/24 ,  B01J20/3212 ,  B01J20/3219 ,  B01J20/3265 ,  B01J20/3293 ,  C07K1/14

Abstract: The purpose of the present invention is to adsorb calciprotein particles. An adsorbent for calciprotein particles of the present invention is characterized in that the surface of a water-insoluble carrier is covalently bonded, through a hydrocarbon group, to at least one selected from the group consisting of amino group, carboxyl group, phosphate group, phosphono group, phosphino group, and thiol group.

公开(公告)号：US20230340425A1

公开(公告)日：2023-10-26

申请号：US18343861

申请日：2023-06-29

Applicant: Boehringer Ingelheim International GmbH

Inventor： Jonathan COFFMAN ,  Jeff GOBY ,  Scott GODFREY ,  Raquel OROZCO ,  Jens Holger VOGEL

IPC: C12N7/00 ,  A61L2/00 ,  C07K1/14 ,  C07K1/16

CPC classification number: C12N7/00 ,  A61L2/0082 ,  C07K1/14 ,  C07K1/16 ,  C12N2710/00063 ,  A61L2202/21

Abstract: Methods for continuously inactivating virus during manufacture of a biological product are provided. The methods include steps of (1) combining (a) a composition including a biological product, and (b) a composition including a virus-inactivation reagent, to obtain (c) a treatment composition having a predetermined property for inactivation of a virus, (2) confirming that the treatment composition exhibits the predetermined property, (3) transferring the treatment composition to a treatment vessel that includes an inlet, an outlet, and a static mixer, the transferring occurring at the inlet, (4) incubating the treatment composition in the treatment vessel at a predetermined temperature while the treatment composition flows at a predetermined rate and contacts the static mixer, and (5) collecting the treatment composition from the treatment vessel at the outlet, wherein steps (1) to (5) are carried out continuously. Apparatuses and systems including such a treatment vessel are also provided.

公开(公告)号：US20230340023A1

公开(公告)日：2023-10-26

申请号：US18193144

申请日：2023-03-30

Applicant: Bausch Health Ireland Limited

Inventor： Kunwar Shailubhai ,  Stephen Comiskey ,  Rong Feng ,  Juncai Bai ,  Ruoping Zhang ,  Jun Jia ,  Junfeng Zhou ,  Qiao Zhao ,  Guoqing Zhang

IPC: C07K7/08 ,  C07K1/14 ,  C12N9/88 ,  A61K38/10 ,  B01D15/42

CPC classification number: C07K7/08 ,  C07K1/14 ,  C12N9/88 ,  C12Y406/01002 ,  A61K38/10 ,  B01D15/424

Abstract: The invention provides processes of purifying a peptide including a GCC agonist sequence selected from the group consisting of SEQ ID NOs: 1-251 described herein. The processes include a solvent exchange step before a freeze-drying (lyophilization) step.