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公开(公告)号：US20170226187A1
公开(公告)日：2017-08-10
申请号：US15494551
申请日：2017-04-24
发明人： Jianhua Cai , Jian Shen , Fan Jiang , Na Li , Wentao Wei , Xiuhong Zeng , Xiaoyan Su , Min Han , Delin Ren , Chen Mao
IPC分类号： C07K14/72 , C07K14/435 , C07K14/47
CPC分类号： C07K14/001 , C07K14/43545 , C07K14/4703 , C07K14/4738 , C07K14/723 , C07K2319/00 , C07K2319/35
摘要： The present invention relates to fusion proteins for the expression of G-protein coupled receptor proteins (GPCR) with the fusion partners, as inserted fragments, from mammalian cells. The fusion partners are from a fragment of APJ protein (“the APJ protein fragment”) or a fragment with homology of more than 90% similarity to the APJ protein fragment; or a fragment of RGS16 protein (the “RGS16 protein fragment”) or a fragment with homology of more than 90% similarity to the RGS16 protein fragment; or the fragment of DNJ protein (the “DNJ protein fragment”) or a fragment with homology of more than 90% similarity to DNJ protein fragment. The fusion expression of GPCR with the above mentioned fusion partners can improve the protein yield and stability when purified from cells. Therefore, these fusion protein partners can be widely used for the study of GPCR proteins.
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公开(公告)号：US10221231B2
公开(公告)日：2019-03-05
申请号：US15494547
申请日：2017-04-24
发明人： Jianhua Cai , Jian Shen , Fan Jiang , Na Li , Wentao Wei , Xiuhong Zeng , Xiaoyan Su , Min Han , Delin Ren , Chen Mao
摘要： The present invention relates to fusion proteins for the expression of G-protein coupled receptor proteins (GPCR) with the fusion partners, as inserted fragments, from mammalian cells. The fusion partners are from a fragment of APJ protein (“the APJ protein fragment”) or a fragment with homology of more than 90% similarity to the APJ protein fragment; or a fragment of RGS16 protein (the “RGS16 protein fragment”) or a fragment with homology of more than 90% similarity to the RGS16 protein fragment; or the fragment of DNJ protein (the “DNJ protein fragment”) or a fragment with homology of more than 90% similarity to DNJ protein fragment. The fusion expression of GPCR with the above mentioned fusion partners can improve the protein yield and stability when purified from cells. Therefore, these fusion protein partners can be widely used for the study of GPCR proteins.
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公开(公告)号：US10106595B2
公开(公告)日：2018-10-23
申请号：US15494551
申请日：2017-04-24
发明人： Jianhua Cai , Jian Shen , Fan Jiang , Na Li , Wentao Wei , Xiuhong Zeng , Xiaoyan Su , Min Han , Delin Ren , Chen Mao
摘要： The present invention relates to fusion proteins for the expression of G-protein coupled receptor proteins (GPCR) with the fusion partners, as inserted fragments, from mammalian cells. The fusion partners are from a fragment of APJ protein (“the APJ protein fragment”) or a fragment with homology of more than 90% similarity to the APJ protein fragment; or a fragment of RGS16 protein (the “RGS16 protein fragment”) or a fragment with homology of more than 90% similarity to the RGS16 protein fragment; or the fragment of DNJ protein (the “DNJ protein fragment”) or a fragment with homology of more than 90% similarity to DNJ protein fragment. The fusion expression of GPCR with the above mentioned fusion partners can improve the protein yield and stability when purified from cells. Therefore, these fusion protein partners can be widely used for the study of GPCR proteins.
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公开(公告)号：US09670252B2
公开(公告)日：2017-06-06
申请号：US14633153
申请日：2015-02-27
发明人： Jianhua Cai , Jian Shen , Fan Jiang , Na Li , Wentao Wei , Xiuhong Zeng , Xiaoyan Su , Min Han , Delin Ren , Chen Mao
IPC分类号： C07K14/705 , C07K14/00 , C07K14/72 , C07K14/435 , C07K14/47
CPC分类号： C07K14/001 , C07K14/43545 , C07K14/4703 , C07K14/4738 , C07K14/723 , C07K2319/00 , C07K2319/35
摘要： The present invention relates to fusion proteins for the expression of G-protein coupled receptor proteins (GPCR) with the fusion partners, as inserted fragments, from mammalian cells. The fusion partners are from a fragment of APJ protein (“the APJ protein fragment”) or a fragment with homology of more than 90% similarity to the APJ protein fragment; or a fragment of RGS16 protein (the “RGS16 protein fragment”) or a fragment with homology of more than 90% similarity to the RGS16 protein fragment; or the fragment of DNJ protein (the “DNJ protein fragment”) or a fragment with homology of more than 90% similarity to DNJ protein fragment. The fusion expression of GPCR with the above mentioned fusion partners can improve the protein yield and stability when purified from cells. Therefore, these fusion protein partners can be widely used for the study of GPCR proteins.
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公开(公告)号：US20170226186A1
公开(公告)日：2017-08-10
申请号：US15494547
申请日：2017-04-24
发明人： Jianhua Cai , Jian Shen , Fan Jiang , Na Li , Wentao Wei , Xiuhong Zeng , Xiaoyan Su , Min Han , Delin Ren , Chen Mao
IPC分类号： C07K14/72 , C07K14/435 , C07K14/47
CPC分类号： C07K14/723 , C07K14/001 , C07K14/43545 , C07K14/4703 , C07K14/4738 , C07K2319/00 , C07K2319/35
摘要： The present invention relates to fusion proteins for the expression of G-protein coupled receptor proteins (GPCR) with the fusion partners, as inserted fragments, from mammalian cells. The fusion partners are from a fragment of APJ protein (“the APJ protein fragment”) or a fragment with homology of more than 90% similarity to the APJ protein fragment; or a fragment of RGS16 protein (the “RGS16 protein fragment”) or a fragment with homology of more than 90% similarity to the RGS16 protein fragment; or the fragment of DNJ protein (the “DNJ protein fragment”) or a fragment with homology of more than 90% similarity to DNJ protein fragment. The fusion expression of GPCR with the above mentioned fusion partners can improve the protein yield and stability when purified from cells. Therefore, these fusion protein partners can be widely used for the study of GPCR proteins.
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