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公开(公告)号：US20170226187A1
公开(公告)日：2017-08-10
申请号：US15494551
申请日：2017-04-24
发明人： Jianhua Cai , Jian Shen , Fan Jiang , Na Li , Wentao Wei , Xiuhong Zeng , Xiaoyan Su , Min Han , Delin Ren , Chen Mao
IPC分类号： C07K14/72 , C07K14/435 , C07K14/47
CPC分类号： C07K14/001 , C07K14/43545 , C07K14/4703 , C07K14/4738 , C07K14/723 , C07K2319/00 , C07K2319/35
摘要： The present invention relates to fusion proteins for the expression of G-protein coupled receptor proteins (GPCR) with the fusion partners, as inserted fragments, from mammalian cells. The fusion partners are from a fragment of APJ protein (“the APJ protein fragment”) or a fragment with homology of more than 90% similarity to the APJ protein fragment; or a fragment of RGS16 protein (the “RGS16 protein fragment”) or a fragment with homology of more than 90% similarity to the RGS16 protein fragment; or the fragment of DNJ protein (the “DNJ protein fragment”) or a fragment with homology of more than 90% similarity to DNJ protein fragment. The fusion expression of GPCR with the above mentioned fusion partners can improve the protein yield and stability when purified from cells. Therefore, these fusion protein partners can be widely used for the study of GPCR proteins.
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公开(公告)号：US20150166609A1
公开(公告)日：2015-06-18
申请号：US14633153
申请日：2015-02-27
发明人： Jianhua CAI , Jian SHEN , Fan JIANG , Na LI , Wentao WEI , Xiuhong ZENG , Xiaoyan SU , Min HAN , Delin REN , Chen MAO
CPC分类号： C07K14/001 , C07K14/43545 , C07K14/4703 , C07K14/4738 , C07K14/723 , C07K2319/00 , C07K2319/35
摘要： The present invention relates to fusion proteins for the expression of G-protein coupled receptor proteins (GPCR) with the fusion partners, as inserted fragments, from mammalian cells. The fusion partners are from a fragment of APJ protein (“the APJ protein fragment”) or a fragment with homology of more than 90% similarity to the APJ protein fragment; or a fragment of RGS16 protein (the “RGS16 protein fragment”) or a fragment with homology of more than 90% similarity to the RGS16 protein fragment; or the fragment of DNJ protein (the “DNJ protein fragment”) or a fragment with homology of more than 90% similarity to DNJ protein fragment. The fusion expression of GPCR with the above mentioned fusion partners can improve the protein yield and stability when purified from cells. Therefore, these fusion protein partners can be widely used for the study of GPCR proteins.
摘要翻译： 本发明涉及用于从作为插入片段的哺乳动物细胞中融合配偶体表达G蛋白偶联受体蛋白（GPCR）的融合蛋白。 融合伙伴来自APJ蛋白片段（“APJ蛋白片段”）或与APJ蛋白质片段具有超过90％相似性同源性的片段; 或RGS16蛋白的片段（“RGS16蛋白片段”）或与RGS16蛋白片段具有超过90％相似性的同源性的片段; 或DNJ蛋白片段（“DNJ蛋白片段”）或与DNJ蛋白质片段具有超过90％相似性的同源性的片段。 当从细胞纯化时，GPCR与上述融合伴侣的融合表达可以提高蛋白质产量和稳定性。 因此，这些融合蛋白伴侣可广泛用于研究GPCR蛋白。
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公开(公告)号：US20170226186A1
公开(公告)日：2017-08-10
申请号：US15494547
申请日：2017-04-24
发明人： Jianhua Cai , Jian Shen , Fan Jiang , Na Li , Wentao Wei , Xiuhong Zeng , Xiaoyan Su , Min Han , Delin Ren , Chen Mao
IPC分类号： C07K14/72 , C07K14/435 , C07K14/47
CPC分类号： C07K14/723 , C07K14/001 , C07K14/43545 , C07K14/4703 , C07K14/4738 , C07K2319/00 , C07K2319/35
摘要： The present invention relates to fusion proteins for the expression of G-protein coupled receptor proteins (GPCR) with the fusion partners, as inserted fragments, from mammalian cells. The fusion partners are from a fragment of APJ protein (“the APJ protein fragment”) or a fragment with homology of more than 90% similarity to the APJ protein fragment; or a fragment of RGS16 protein (the “RGS16 protein fragment”) or a fragment with homology of more than 90% similarity to the RGS16 protein fragment; or the fragment of DNJ protein (the “DNJ protein fragment”) or a fragment with homology of more than 90% similarity to DNJ protein fragment. The fusion expression of GPCR with the above mentioned fusion partners can improve the protein yield and stability when purified from cells. Therefore, these fusion protein partners can be widely used for the study of GPCR proteins.
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4.发明申请公开(公告)号：US20170159096A1
公开(公告)日：2017-06-08
申请号：US15362826
申请日：2016-11-29
发明人： Dongming Qian , Jianhua Cai , Liang Hu , Ping Wang , Lirong Wang , Xiaobo Yu , Danjing Wang , Xiuhong Zeng , Chen Mao , Delin Ren
CPC分类号： C12P21/02 , C07K14/47 , C07K14/4702 , C07K14/705 , C07K19/00 , C07K2319/00 , C12N15/62 , C12N15/85 , C12N15/866
摘要： The present invention provides a protein fragment for fusion expression of ion channel protein and transport protein. The protein fragment is a Bril protein fragment or a T4L protein fragment. Also provided is a method for preparing a fusion protein by inserting a Brit protein fragment or T4L protein fragment into the N-terminal, C-terminal or intramembrane loop region of the ion channel protein and transport protein so as to improve its, in-vitro stability and crystallizability.
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公开(公告)号：US10221231B2
公开(公告)日：2019-03-05
申请号：US15494547
申请日：2017-04-24
发明人： Jianhua Cai , Jian Shen , Fan Jiang , Na Li , Wentao Wei , Xiuhong Zeng , Xiaoyan Su , Min Han , Delin Ren , Chen Mao
摘要： The present invention relates to fusion proteins for the expression of G-protein coupled receptor proteins (GPCR) with the fusion partners, as inserted fragments, from mammalian cells. The fusion partners are from a fragment of APJ protein (“the APJ protein fragment”) or a fragment with homology of more than 90% similarity to the APJ protein fragment; or a fragment of RGS16 protein (the “RGS16 protein fragment”) or a fragment with homology of more than 90% similarity to the RGS16 protein fragment; or the fragment of DNJ protein (the “DNJ protein fragment”) or a fragment with homology of more than 90% similarity to DNJ protein fragment. The fusion expression of GPCR with the above mentioned fusion partners can improve the protein yield and stability when purified from cells. Therefore, these fusion protein partners can be widely used for the study of GPCR proteins.
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公开(公告)号：US10106595B2
公开(公告)日：2018-10-23
申请号：US15494551
申请日：2017-04-24
发明人： Jianhua Cai , Jian Shen , Fan Jiang , Na Li , Wentao Wei , Xiuhong Zeng , Xiaoyan Su , Min Han , Delin Ren , Chen Mao
摘要： The present invention relates to fusion proteins for the expression of G-protein coupled receptor proteins (GPCR) with the fusion partners, as inserted fragments, from mammalian cells. The fusion partners are from a fragment of APJ protein (“the APJ protein fragment”) or a fragment with homology of more than 90% similarity to the APJ protein fragment; or a fragment of RGS16 protein (the “RGS16 protein fragment”) or a fragment with homology of more than 90% similarity to the RGS16 protein fragment; or the fragment of DNJ protein (the “DNJ protein fragment”) or a fragment with homology of more than 90% similarity to DNJ protein fragment. The fusion expression of GPCR with the above mentioned fusion partners can improve the protein yield and stability when purified from cells. Therefore, these fusion protein partners can be widely used for the study of GPCR proteins.
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公开(公告)号：US09670252B2
公开(公告)日：2017-06-06
申请号：US14633153
申请日：2015-02-27
发明人： Jianhua Cai , Jian Shen , Fan Jiang , Na Li , Wentao Wei , Xiuhong Zeng , Xiaoyan Su , Min Han , Delin Ren , Chen Mao
IPC分类号： C07K14/705 , C07K14/00 , C07K14/72 , C07K14/435 , C07K14/47
CPC分类号： C07K14/001 , C07K14/43545 , C07K14/4703 , C07K14/4738 , C07K14/723 , C07K2319/00 , C07K2319/35
摘要： The present invention relates to fusion proteins for the expression of G-protein coupled receptor proteins (GPCR) with the fusion partners, as inserted fragments, from mammalian cells. The fusion partners are from a fragment of APJ protein (“the APJ protein fragment”) or a fragment with homology of more than 90% similarity to the APJ protein fragment; or a fragment of RGS16 protein (the “RGS16 protein fragment”) or a fragment with homology of more than 90% similarity to the RGS16 protein fragment; or the fragment of DNJ protein (the “DNJ protein fragment”) or a fragment with homology of more than 90% similarity to DNJ protein fragment. The fusion expression of GPCR with the above mentioned fusion partners can improve the protein yield and stability when purified from cells. Therefore, these fusion protein partners can be widely used for the study of GPCR proteins.
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