公开(公告)号：US20170226187A1

公开(公告)日：2017-08-10

申请号：US15494551

申请日：2017-04-24

申请人： VIVA BIOTECH (SHANGHAI) LTD.

发明人： Jianhua Cai ,  Jian Shen ,  Fan Jiang ,  Na Li ,  Wentao Wei ,  Xiuhong Zeng ,  Xiaoyan Su ,  Min Han ,  Delin Ren ,  Chen Mao

IPC分类号： C07K14/72 ,  C07K14/435 ,  C07K14/47

CPC分类号： C07K14/001 ,  C07K14/43545 ,  C07K14/4703 ,  C07K14/4738 ,  C07K14/723 ,  C07K2319/00 ,  C07K2319/35

摘要： The present invention relates to fusion proteins for the expression of G-protein coupled receptor proteins (GPCR) with the fusion partners, as inserted fragments, from mammalian cells. The fusion partners are from a fragment of APJ protein (“the APJ protein fragment”) or a fragment with homology of more than 90% similarity to the APJ protein fragment; or a fragment of RGS16 protein (the “RGS16 protein fragment”) or a fragment with homology of more than 90% similarity to the RGS16 protein fragment; or the fragment of DNJ protein (the “DNJ protein fragment”) or a fragment with homology of more than 90% similarity to DNJ protein fragment. The fusion expression of GPCR with the above mentioned fusion partners can improve the protein yield and stability when purified from cells. Therefore, these fusion protein partners can be widely used for the study of GPCR proteins.

公开(公告)号：US20170226186A1

公开(公告)日：2017-08-10

申请号：US15494547

申请日：2017-04-24

申请人： VIVA BIOTECH (SHANGHAI) LTD.

发明人： Jianhua Cai ,  Jian Shen ,  Fan Jiang ,  Na Li ,  Wentao Wei ,  Xiuhong Zeng ,  Xiaoyan Su ,  Min Han ,  Delin Ren ,  Chen Mao

IPC分类号： C07K14/72 ,  C07K14/435 ,  C07K14/47

CPC分类号： C07K14/723 ,  C07K14/001 ,  C07K14/43545 ,  C07K14/4703 ,  C07K14/4738 ,  C07K2319/00 ,  C07K2319/35

摘要： The present invention relates to fusion proteins for the expression of G-protein coupled receptor proteins (GPCR) with the fusion partners, as inserted fragments, from mammalian cells. The fusion partners are from a fragment of APJ protein (“the APJ protein fragment”) or a fragment with homology of more than 90% similarity to the APJ protein fragment; or a fragment of RGS16 protein (the “RGS16 protein fragment”) or a fragment with homology of more than 90% similarity to the RGS16 protein fragment; or the fragment of DNJ protein (the “DNJ protein fragment”) or a fragment with homology of more than 90% similarity to DNJ protein fragment. The fusion expression of GPCR with the above mentioned fusion partners can improve the protein yield and stability when purified from cells. Therefore, these fusion protein partners can be widely used for the study of GPCR proteins.

公开(公告)号：US20170159096A1

公开(公告)日：2017-06-08

申请号：US15362826

申请日：2016-11-29

申请人： VIVA Biotech (Shanghai) Ltd.

发明人： Dongming Qian ,  Jianhua Cai ,  Liang Hu ,  Ping Wang ,  Lirong Wang ,  Xiaobo Yu ,  Danjing Wang ,  Xiuhong Zeng ,  Chen Mao ,  Delin Ren

IPC分类号： C12P21/02 ,  C07K14/47

CPC分类号： C12P21/02 ,  C07K14/47 ,  C07K14/4702 ,  C07K14/705 ,  C07K19/00 ,  C07K2319/00 ,  C12N15/62 ,  C12N15/85 ,  C12N15/866

摘要： The present invention provides a protein fragment for fusion expression of ion channel protein and transport protein. The protein fragment is a Bril protein fragment or a T4L protein fragment. Also provided is a method for preparing a fusion protein by inserting a Brit protein fragment or T4L protein fragment into the N-terminal, C-terminal or intramembrane loop region of the ion channel protein and transport protein so as to improve its, in-vitro stability and crystallizability.

公开(公告)号：US10221231B2

公开(公告)日：2019-03-05

申请号：US15494547

申请日：2017-04-24

申请人： VIVA BIOTECH (SHANGHAI) LTD.

发明人： Jianhua Cai ,  Jian Shen ,  Fan Jiang ,  Na Li ,  Wentao Wei ,  Xiuhong Zeng ,  Xiaoyan Su ,  Min Han ,  Delin Ren ,  Chen Mao

IPC分类号： C12N5/10 ,  C12N15/63 ,  C07K14/72 ,  C07K14/00 ,  C07K14/435 ,  C07K14/47

摘要： The present invention relates to fusion proteins for the expression of G-protein coupled receptor proteins (GPCR) with the fusion partners, as inserted fragments, from mammalian cells. The fusion partners are from a fragment of APJ protein (“the APJ protein fragment”) or a fragment with homology of more than 90% similarity to the APJ protein fragment; or a fragment of RGS16 protein (the “RGS16 protein fragment”) or a fragment with homology of more than 90% similarity to the RGS16 protein fragment; or the fragment of DNJ protein (the “DNJ protein fragment”) or a fragment with homology of more than 90% similarity to DNJ protein fragment. The fusion expression of GPCR with the above mentioned fusion partners can improve the protein yield and stability when purified from cells. Therefore, these fusion protein partners can be widely used for the study of GPCR proteins.

公开(公告)号：US10106595B2

公开(公告)日：2018-10-23

申请号：US15494551

申请日：2017-04-24

申请人： VIVA BIOTECH (SHANGHAI) LTD.

发明人： Jianhua Cai ,  Jian Shen ,  Fan Jiang ,  Na Li ,  Wentao Wei ,  Xiuhong Zeng ,  Xiaoyan Su ,  Min Han ,  Delin Ren ,  Chen Mao

IPC分类号： C12N15/12 ,  C12N15/62 ,  C12N15/63 ,  C07K14/72 ,  C07K14/47 ,  C07K14/435

摘要： The present invention relates to fusion proteins for the expression of G-protein coupled receptor proteins (GPCR) with the fusion partners, as inserted fragments, from mammalian cells. The fusion partners are from a fragment of APJ protein (“the APJ protein fragment”) or a fragment with homology of more than 90% similarity to the APJ protein fragment; or a fragment of RGS16 protein (the “RGS16 protein fragment”) or a fragment with homology of more than 90% similarity to the RGS16 protein fragment; or the fragment of DNJ protein (the “DNJ protein fragment”) or a fragment with homology of more than 90% similarity to DNJ protein fragment. The fusion expression of GPCR with the above mentioned fusion partners can improve the protein yield and stability when purified from cells. Therefore, these fusion protein partners can be widely used for the study of GPCR proteins.

公开(公告)号：US09670252B2

公开(公告)日：2017-06-06

申请号：US14633153

申请日：2015-02-27

申请人： Viva Biotech (Shanghai) Ltd.

发明人： Jianhua Cai ,  Jian Shen ,  Fan Jiang ,  Na Li ,  Wentao Wei ,  Xiuhong Zeng ,  Xiaoyan Su ,  Min Han ,  Delin Ren ,  Chen Mao

IPC分类号： C07K14/705 ,  C07K14/00 ,  C07K14/72 ,  C07K14/435 ,  C07K14/47

CPC分类号： C07K14/001 ,  C07K14/43545 ,  C07K14/4703 ,  C07K14/4738 ,  C07K14/723 ,  C07K2319/00 ,  C07K2319/35

摘要： The present invention relates to fusion proteins for the expression of G-protein coupled receptor proteins (GPCR) with the fusion partners, as inserted fragments, from mammalian cells. The fusion partners are from a fragment of APJ protein (“the APJ protein fragment”) or a fragment with homology of more than 90% similarity to the APJ protein fragment; or a fragment of RGS16 protein (the “RGS16 protein fragment”) or a fragment with homology of more than 90% similarity to the RGS16 protein fragment; or the fragment of DNJ protein (the “DNJ protein fragment”) or a fragment with homology of more than 90% similarity to DNJ protein fragment. The fusion expression of GPCR with the above mentioned fusion partners can improve the protein yield and stability when purified from cells. Therefore, these fusion protein partners can be widely used for the study of GPCR proteins.