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公开(公告)号:US08119382B2
公开(公告)日:2012-02-21
申请号:US12065384
申请日:2006-08-18
IPC分类号: C12N9/22
摘要: Methods are provided for making restriction endonucleases with reduced star activity by one or more targeted mutations to a catalytic site within the restriction endonuclease. Examples of modifications to restriction endonucleases with significant sequence identity with KpnI are provided and reduced star activity demonstrated.
摘要翻译: 提供了用于通过对限制性内切核酸酶内的催化位点的一个或多个靶向突变来制备具有降低的星形活性的限制性内切核酸酶的方法。 提供了与KpnI具有显着序列同一性的限制性内切核酸酶的修饰实例,并显示降低的星形活性。
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公开(公告)号:US20080227133A1
公开(公告)日:2008-09-18
申请号:US12065384
申请日:2006-08-18
摘要: Methods are provided for making restriction endonucleases with reduced star activity by one or more targeted mutations to a catalytic site within the restriction endonuclease. Examples of modifications to restriction endonucleases with significant sequence identity with KpnI are provided and reduced star activity demonstrated.
摘要翻译: 提供了用于通过对限制性内切核酸酶内的催化位点的一个或多个靶向突变来制备具有降低的星形活性的限制性内切核酸酶的方法。 提供了与KpnI具有显着序列同一性的限制性内切核酸酶的修饰实例,并显示降低的星形活性。
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3.
公开(公告)号:US20130130293A1
公开(公告)日:2013-05-23
申请号:US13346798
申请日:2012-01-10
IPC分类号: C12N9/16
摘要: Methods are provided for making restriction endonucleases with reduced star activity by one or more targeted mutations to a catalytic site within the restriction endonuclease. Examples of modifications to restriction endonucleases with significant sequence identity with KpnI are provided and reduced star activity demonstrated.
摘要翻译: 提供了用于通过对限制性内切核酸酶内的催化位点的一个或多个靶向突变来制备具有降低的星形活性的限制性内切核酸酶的方法。 提供了与KpnI具有显着序列同一性的限制性内切核酸酶的修饰实例,并显示降低的星形活性。
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