Variant of KpnI restriction endonuclease
    1.
    发明授权
    Variant of KpnI restriction endonuclease 有权
    KpnI限制性内切核酸酶的变体

    公开(公告)号:US08119382B2

    公开(公告)日:2012-02-21

    申请号:US12065384

    申请日:2006-08-18

    IPC分类号: C12N9/22

    CPC分类号: C12N9/16 C12N9/22

    摘要: Methods are provided for making restriction endonucleases with reduced star activity by one or more targeted mutations to a catalytic site within the restriction endonuclease. Examples of modifications to restriction endonucleases with significant sequence identity with KpnI are provided and reduced star activity demonstrated.

    摘要翻译: 提供了用于通过对限制性内切核酸酶内的催化位点的一个或多个靶向突变来制备具有降低的星形活性的限制性内切核酸酶的方法。 提供了与KpnI具有显着序列同一性的限制性内切核酸酶的修饰实例,并显示降低的星形活性。

    Method for Reducing Star Activity in Restriction Endonucleases
    3.
    发明申请
    Method for Reducing Star Activity in Restriction Endonucleases 审中-公开
    限制性内切核酸酶降低星形活性的方法

    公开(公告)号:US20130130293A1

    公开(公告)日:2013-05-23

    申请号:US13346798

    申请日:2012-01-10

    IPC分类号: C12N9/16

    CPC分类号: C12N9/16 C12N9/22

    摘要: Methods are provided for making restriction endonucleases with reduced star activity by one or more targeted mutations to a catalytic site within the restriction endonuclease. Examples of modifications to restriction endonucleases with significant sequence identity with KpnI are provided and reduced star activity demonstrated.

    摘要翻译: 提供了用于通过对限制性内切核酸酶内的催化位点的一个或多个靶向突变来制备具有降低的星形活性的限制性内切核酸酶的方法。 提供了与KpnI具有显着序列同一性的限制性内切核酸酶的修饰实例,并显示降低的星形活性。