公开(公告)号：US20120301881A1

公开(公告)日：2012-11-29

申请号：US13522224

申请日：2011-01-20

申请人： Zhenyu Zhu ,  Yu Zheng ,  Shengxi Guan ,  Hua Wang ,  Aine Quimby ,  Penghua Zhang ,  Lynne Apone

发明人： Zhenyu Zhu ,  Yu Zheng ,  Shengxi Guan ,  Hua Wang ,  Aine Quimby ,  Penghua Zhang ,  Lynne Apone

IPC分类号： C12Q1/68 ,  C12N9/16

CPC分类号： C12N9/22 ,  C12Q1/6827 ,  C12Q2521/331

摘要： Compositions, methods and a kit are described relating to a novel family of enzymes which preferentially bind to a hydroxymethylated cytosine or a glucosylated hydroxymethylated cytosine and cleave double-stranded DNA at a defined distance 3′ of the recognition site to produce a cleavage fragment with a 1-3 base overhang.

摘要翻译： 描述了组合物，方法和试剂盒，其涉及一种新型家族的酶，其优先结合羟甲基化的胞嘧啶或葡糖基化的羟甲基化的胞嘧啶，并在识别位点的3'的限定距离处切割双链DNA以产生具有 1-3基础突出。

公开(公告)号：US06723546B2

公开(公告)日：2004-04-20

申请号：US10106275

申请日：2002-03-26

申请人： Zhenyu Zhu ,  Shuang-Yong Xu

发明人： Zhenyu Zhu ,  Shuang-Yong Xu

IPC分类号： C12N922

CPC分类号： C12N9/1007 ,  C12N9/22

摘要： The present invention relates to recombinant DNA which encodes the BsaI restriction endonuclease as well as BsaI methylase, expression of BsaI restriction endonuclease and BsaI methylase in E. coli cells containing the recombinant DNA, and purification of BsaI restriction endonuclease to near homogeneity.

摘要翻译： 本发明涉及编码BsaI限制性内切核酸酶以及BsaI甲基化酶的重组DNA，BsaI限制性内切核酸酶和BsaI甲基化酶在含有重组DNA的大肠杆菌细胞中的表达，并将BsaI限制性内切核酸酶纯化至接近同质性。

公开(公告)号：US20190227753A1

公开(公告)日：2019-07-25

申请号：US16367608

申请日：2019-03-28

申请人： Zhenyu Zhu ,  Mikal Hunsaker ,  Karthi R. Vadivelu ,  Rahul Bhatt ,  Kenneth P. Foust ,  Rajesh Bhaskar ,  Amit Kumar Srivastava

发明人： Zhenyu Zhu ,  Mikal Hunsaker ,  Karthi R. Vadivelu ,  Rahul Bhatt ,  Kenneth P. Foust ,  Rajesh Bhaskar ,  Amit Kumar Srivastava

IPC分类号： G06F3/06 ,  G06F13/38 ,  G06F9/54

摘要： In one embodiment, a flash sharing controller is to enable a plurality of components of a platform to share a flash memory. The flash sharing controller may include: a flash sharing class layer including a configuration controller to configure the plurality of components to be flash master devices and configure a flash sharing slave device for the flash memory; and a physical layer coupled to the flash sharing class layer to communicate with the plurality of components via a bus. Other embodiments are described and claimed.

公开(公告)号：US08969061B2

公开(公告)日：2015-03-03

申请号：US13522224

申请日：2011-01-20

申请人： Zhenyu Zhu ,  Yu Zheng ,  Shengxi Guan ,  Hua Wang ,  Aine Quimby ,  Penghua Zhang ,  Lynne Apone

发明人： Zhenyu Zhu ,  Yu Zheng ,  Shengxi Guan ,  Hua Wang ,  Aine Quimby ,  Penghua Zhang ,  Lynne Apone

IPC分类号： C12Q1/00 ,  C12Q1/68 ,  C12Q1/34 ,  C12N9/00 ,  C12N9/22

CPC分类号： C12N9/22 ,  C12Q1/6827 ,  C12Q2521/331

摘要： Compositions, methods and a kit are described relating to a novel family of enzymes which preferentially bind to a hydroxymethylated cytosine or a glucosylated hydroxymethylated cytosine and cleave double-stranded DNA at a defined distance 3′ of the recognition site to produce a cleavage fragment with a 1-3 base overhang.

摘要翻译： 描述了组合物，方法和试剂盒，其涉及一种新型家族的酶，其优先结合羟甲基化的胞嘧啶或葡糖基化的羟甲基化的胞嘧啶，并在识别位点的3'的限定距离处切割双链DNA以产生具有 1-3基础突出。

公开(公告)号：US20130130293A1

公开(公告)日：2013-05-23

申请号：US13346798

申请日：2012-01-10

申请人： Valakunja Nagaraja ,  Matheshwaran Saravanan ,  Zhenyu Zhu

发明人： Valakunja Nagaraja ,  Matheshwaran Saravanan ,  Zhenyu Zhu

IPC分类号： C12N9/16

CPC分类号： C12N9/16 ,  C12N9/22

摘要： Methods are provided for making restriction endonucleases with reduced star activity by one or more targeted mutations to a catalytic site within the restriction endonuclease. Examples of modifications to restriction endonucleases with significant sequence identity with KpnI are provided and reduced star activity demonstrated.

摘要翻译： 提供了用于通过对限制性内切核酸酶内的催化位点的一个或多个靶向突变来制备具有降低的星形活性的限制性内切核酸酶的方法。 提供了与KpnI具有显着序列同一性的限制性内切核酸酶的修饰实例，并显示降低的星形活性。

公开(公告)号：US20120088237A1

公开(公告)日：2012-04-12

申请号：US13202851

申请日：2010-03-04

申请人： Zhenyu Zhu ,  Shengxi Guan ,  Aine Quimby

发明人： Zhenyu Zhu ,  Shengxi Guan ,  Aine Quimby

IPC分类号： C12Q1/68 ,  C12N9/16 ,  C12N1/21 ,  C12N15/55 ,  C12N15/63

CPC分类号： C12N9/22

摘要： A restriction endonuclease is provided that has been engineered to have a cleavage specificity for a DNA recognition sequence containing a modified nucleotide. Methods for engineering enzymes to cleave DNA containing modified nucleotides at specific sequences are provided.

摘要翻译： 提供了限制性内切核酸酶，其被工程化以具有对含有修饰的核苷酸的DNA识别序列的切割特异性。 提供了用于以特定序列切割含有修饰的核苷酸的DNA的工程酶的方法。

公开(公告)号：US20080268507A1

公开(公告)日：2008-10-30

申请号：US11666148

申请日：2005-10-21

申请人： Shuang-yong Xu ,  Zhenyu Zhu ,  Shi-hong Chan ,  Yan Xu

发明人： Shuang-yong Xu ,  Zhenyu Zhu ,  Shi-hong Chan ,  Yan Xu

IPC分类号： C12P19/34 ,  C07H21/04 ,  C12N9/16 ,  C12N9/10 ,  C12N15/00 ,  C12N1/21

CPC分类号： C12N9/22 ,  C12N9/1241

摘要： Recombinant nicking endonucleases and associated methylases have been obtained and sequenced and their specificity has been defined. A mutant form of the nicking endonuclease has been cloned where the mutation includes deletion of amino acid sequences at the C-terminal end of the protein. The nicking enzymes have been used for a number of purposes including: amplifying DNA from as few cells as can be found in a single bacterial colony in the presence of a strand displacing polymerase; and for removing genomic DNA in a biological preparation where it is deemed to be a contaminant.

摘要翻译： 已经获得重组切口内切核酸酶和相关的甲基化酶并测序并确定了它们的特异性。 已经克隆了缺口内切核酸酶的突变形式，其中突变包括在蛋白质C末端缺失氨基酸序列。 切口酶已经被用于许多目的，包括：在链置换聚合酶存在下，从单个细菌菌落中可以发现的细胞中扩增DNA; 并且用于在被认为是污染物的生物制剂中去除基因组DNA。

公开(公告)号：US20080213860A1

公开(公告)日：2008-09-04

申请号：US11631438

申请日：2005-07-22

申请人： Shuang-Yong Xu ,  Zhenyu Zhu ,  Timothy Meixsell

发明人： Shuang-Yong Xu ,  Zhenyu Zhu ,  Timothy Meixsell

IPC分类号： C12N9/14

CPC分类号： C12N9/22

摘要： A nicking endonuclease is described which has an amino acid sequence with at least 70% identity to SEQ ID NO:6 and comprising a mutation at least one of an arginine or gutamic acid corresponding to position 507 and position 546 respectively in SEQ ID NO:6.

摘要翻译： 描述了一种切口内切核酸酶，其具有与SEQ ID NO：6具有至少70％同一性的氨基酸序列，并且包含分别对应于SEQ ID NO：6中的位置507和位置546的精氨酸或谷氨酸中的至少一种的突变 。

公开(公告)号：US20190258539A1

公开(公告)日：2019-08-22

申请号：US16398076

申请日：2019-04-29

申请人： Zhenyu Zhu ,  William A. Stevens, JR. ,  Michael T. Klinglesmith ,  Mikal Hunsaker

发明人： Zhenyu Zhu ,  William A. Stevens, JR. ,  Michael T. Klinglesmith ,  Mikal Hunsaker

IPC分类号： G06F11/10 ,  G06F13/42 ,  G06F13/16

摘要： Embodiments include a serial bus controller that may be coupled to an in band serial peripheral interface (SPI) link, to request a write of data and a subsequent read of the data from a slave device and in response to the request to read the data, receive a bit error report and optionally correct the bit error over the in band SPI link. Embodiments include a slave device, e.g., a flash memory device, to detect and report the bit error over the in band SPI link, where the flash memory device, in response to a request to write and/or erase data, calculates or determines an error correction code (ECC) and stores corresponding parity data. In embodiments, after receiving a subsequent request to read the data, the flash memory device accesses the stored parity data to check the ECC for a bit error and if a bit error is detected, reports the detected bit error over the in band SPI link. Other embodiments may be described and claimed.

公开(公告)号：US09026712B2

公开(公告)日：2015-05-05

申请号：US13532730

申请日：2012-06-25

申请人： Piotr Kwidzinski ,  Zhenyu Zhu

发明人： Piotr Kwidzinski ,  Zhenyu Zhu

IPC分类号： G06F21/85

CPC分类号： G06F21/85 ,  G06F13/126 ,  G06F13/426

摘要： Described herein are embodiments of USB device control using endpoint type detection during enumeration. An apparatus configured for USB device control using endpoint type detection during enumeration may include a host controller configured to selectively disable enumeration of a USB device based at least in part on an endpoint type of the USB device. The apparatus may include a management engine configured to store in the host controller a USB lock policy defining endpoint types disallowed to be enumerated by the apparatus. Other embodiments may be described and/or claimed.

摘要翻译： 这里描述的是在枚举期间使用端点类型检测的USB设备控制的实施例。 配置用于在枚举期间使用端点类型检测的USB设备控制的设备可以包括被配置为至少部分地基于USB设备的端点类型来选择性地禁用USB设备的枚举的主机控制器。 该设备可以包括管理引擎，其被配置为在主机控制器中存储定义不允许被该设备枚举的端点类型的USB锁定策略。 可以描述和/或要求保护其他实施例。