公开(公告)号：US06987091B2

公开(公告)日：2006-01-17

申请号：US10301514

申请日：2002-11-21

申请人： Vern L. Schramm ,  Jef D. Boeke ,  Anthony Sauvé ,  Ivana Celic

发明人： Vern L. Schramm ,  Jef D. Boeke ,  Anthony Sauvé ,  Ivana Celic

IPC分类号： C07H19/04 ,  C12P19/36 ,  C07F9/6512

CPC分类号： C12N9/1077 ,  C07H19/04 ,  C07H19/20 ,  C12N9/99 ,  C12P19/32

摘要： A novel compound, 2′/3′-O-acetyl-ADP-ribose, is provided. The compound is a mixture of the 2′ and 3′ regioisomers of O-acetyl-ADP ribose, and is formed nonenzymatically from 2′-O-acetyl-ADP-ribose, which is the newly discovered product of the reaction of Sir2 enzymes with acetylated peptides and NAD+. Analogs of 2′/3′-O-acetyl-ADP-ribose are also provided. Additionally, methods of preparing 2′/3′-O-acetyl-ADP-ribose, methods of determining whether a test compound is an inhibitor of a Sir2 enzyme, methods of detecting Sir2 activity in a composition, methods of deacetylating an acetylated peptide, and methods of inhibiting the deacetylation of an acetylated peptide are provided. Prodrugs of 2′/3′-O-acetyl-ADP-ribose are also provided.

公开(公告)号：US07741295B2

公开(公告)日：2010-06-22

申请号：US12231880

申请日：2008-09-05

申请人： Vern L. Schramm ,  Jef D. Boeke ,  Anthony Sauvé ,  Ivana Celic

发明人： Vern L. Schramm ,  Jef D. Boeke ,  Anthony Sauvé ,  Ivana Celic

IPC分类号： A61K31/70 ,  A01N43/04

CPC分类号： C12N9/1077 ,  C07H19/04 ,  C07H19/20 ,  C12N9/99 ,  C12P19/32

摘要： A novel compound, 2′/3′-O-acetyl-ADP-ribose, is provided. The compound is a mixture of the 2′ and 3′ regioisomers of O-acetyl-ADP ribose, and is formed nonenzymatically from 2′-O-acetyl-ADP-ribose, which is the newly discovered product of the reaction of Sir2 enzymes with acetylated peptides and NAD+. Analogs of 2′/3′-O-acetyl-ADP-ribose are also provided. Additionally, methods of preparing 2′/3′-O-acetyl-ADP-ribose, methods of determining whether a test compound is an inhibitor of a Sir2 enzyme, methods of detecting Sir2 activity in a composition, methods of deacetylating an acetylated peptide, and methods of inhibiting the deacetylation of an acetylated peptide are provided. Prodrugs of 2′/3′-O-acetyl-ADP-ribose are also provided.

摘要翻译： 提供新的化合物2'/ 3'-O-乙酰基-ADP-核糖。 该化合物是O-乙酰基-ADP核糖的2'和3'区域异构体的混合物，并且由2'-O-乙酰基-ADP-核糖非酶催化形成，其是Sir2酶与 乙酰化肽和NAD +。 还提供了2'/ 3'-O-乙酰基-ADP-核糖的类似物。 另外，制备2'/ 3'-O-乙酰基-ADP-核糖的方法，确定测试化合物是否为Sir2酶抑制剂的方法，组合物中Sir2活性检测方法，乙酰化肽脱乙酰化的方法， 并提供了抑制乙酰化肽脱乙酰化的方法。 还提供了2'/ 3'-O-乙酰基-ADP-核糖的前药。

公开(公告)号：US07432246B2

公开(公告)日：2008-10-07

申请号：US11248523

申请日：2005-10-12

申请人： Vern L. Schramm ,  Jef D. Boeke ,  Anthony Sauvé ,  Ivana Celic

发明人： Vern L. Schramm ,  Jef D. Boeke ,  Anthony Sauvé ,  Ivana Celic

IPC分类号： C07H19/04 ,  C12P19/36 ,  C07F9/6512 ,  C07H21/02 ,  C07H21/04

CPC分类号： C12N9/1077 ,  C07H19/04 ,  C07H19/20 ,  C12N9/99 ,  C12P19/32

摘要： A novel compound, 2′/3′-O-acetyl-ADP-ribose, is provided. The compound is a mixture of the 2′ and 3′ regioisomers of O-acetyl-ADP ribose, and is formed nonenzymatically from 2′-O-acetyl-ADP-ribose, which is the newly discovered product of the reaction of Sir2 enzymes with acetylated peptides and NAD+. Analogs of 2′/3′-O-acetyl-ADP-ribose are also provided. Additionally, methods of preparing 2′/3′-O-acetyl-ADP-ribose, methods of determining whether a test compound is an inhibitor of a Sir2 enzyme, methods of detecting Sir2 activity in a composition, methods of deacetylating an acetylated peptide, and methods of inhibiting the deacetylation of an acetylated peptide are provided. Prodrugs of 2′/3′-O-acetyl-ADP-ribose are also provided.

摘要翻译： 提供新的化合物2'/ 3'-O-乙酰基-ADP-核糖。 该化合物是O-乙酰基-ADP核糖的2'和3'区域异构体的混合物，并且由2'-O-乙酰基-ADP-核糖非酶催化形成，其是Sir2酶与 乙酰化肽和NAD +。 还提供了2'/ 3'-O-乙酰基-ADP-核糖的类似物。 另外，制备2'/ 3'-O-乙酰基-ADP-核糖的方法，确定测试化合物是否为Sir2酶抑制剂的方法，组合物中Sir2活性检测方法，乙酰化肽脱乙酰化的方法， 并提供了抑制乙酰化肽脱乙酰化的方法。 还提供了2'/ 3'-O-乙酰基-ADP-核糖的前药。

公开(公告)号：US20090068695A1

公开(公告)日：2009-03-12

申请号：US12231880

申请日：2008-09-05

申请人： Vern L. Schramm ,  Jef D. Boeke ,  Anthony Sauve ,  Ivana Celic

发明人： Vern L. Schramm ,  Jef D. Boeke ,  Anthony Sauve ,  Ivana Celic

IPC分类号： C12Q1/34

CPC分类号： C12N9/1077 ,  C07H19/04 ,  C07H19/20 ,  C12N9/99 ,  C12P19/32

摘要： A novel compound, 2′/3′-O-acetyl-ADP-ribose, is provided. The compound is a mixture of the 2′ and 3′ regioisomers of O-acetyl-ADP ribose, and is formed nonenzymatically from 2′-O-acetyl-ADP-ribose, which is the newly discovered product of the reaction of Sir2 enzymes with acetylated peptides and NAD+. Analogs of 2′/3′-O-acetyl-ADP-ribose are also provided. Additionally, methods of preparing 2′/3′-O-acetyl-ADP-ribose, methods of determining whether a test compound is an inhibitor of a Sir2 enzyme, methods of detecting Sir2 activity in a composition, methods of deacetylating an acetylated peptide, and methods of inhibiting the deacetylation of an acetylated peptide are provided. Prodrugs of 2′/3′-O-acetyl-ADP-ribose are also provided.

摘要翻译： 提供新的化合物2'/ 3'-O-乙酰基-ADP-核糖。 该化合物是O-乙酰基-ADP核糖的2'和3'区域异构体的混合物，并且由2'-O-乙酰基-ADP-核糖非酶催化形成，其是Sir2酶与 乙酰化肽和NAD +。 还提供了2'/ 3'-O-乙酰基-ADP-核糖的类似物。 另外，制备2'/ 3'-O-乙酰基-ADP-核糖的方法，确定测试化合物是否为Sir2酶抑制剂的方法，组合物中Sir2活性检测方法，乙酰化肽脱乙酰化的方法， 并提供了抑制乙酰化肽脱乙酰化的方法。 还提供了2'/ 3'-O-乙酰基-ADP-核糖的前药。

公开(公告)号：US09242245B2

公开(公告)日：2016-01-26

申请号：US13497871

申请日：2010-09-21

申请人： Alex Leo Kolodkin ,  Terry Harrison Shelley ,  Shih-Hsiu Wang ,  Ivana Celic

发明人： Alex Leo Kolodkin ,  Terry Harrison Shelley ,  Shih-Hsiu Wang ,  Ivana Celic

IPC分类号： G01N1/30 ,  B01L3/00

CPC分类号： B01L3/50255 ,  B01L2300/0851

摘要： A floating chamber set may include a plurality of containers coupled to a frame such that each container translates vertically within a limited vertical range independent of the other containers. Each container may be perforated to allow liquid penetration.

摘要翻译： 浮动室组可以包括耦合到框架的多个容器，使得每个容器在独立于其它容器的有限的垂直范围内垂直地平移。 每个容器可以穿孔以允许液体渗透。

公开(公告)号：US20120208207A1

公开(公告)日：2012-08-16

申请号：US13497871

申请日：2010-09-21

申请人： Alex Leo Kolodkin ,  Terry Harrison Shelley ,  Shih-Hsiu Wang ,  Ivana Celic

发明人： Alex Leo Kolodkin ,  Terry Harrison Shelley ,  Shih-Hsiu Wang ,  Ivana Celic

IPC分类号： G01N33/53 ,  B01L3/00

CPC分类号： B01L3/50255 ,  B01L2300/0851

摘要： A floating chamber set may include a plurality of containers coupled to a frame such that each container translates vertically within a limited vertical range independent of the other containers. Each container may be perforated to allow liquid penetration.

摘要翻译： 浮动室组可以包括耦合到框架的多个容器，使得每个容器在独立于其它容器的有限的垂直范围内垂直地平移。 每个容器可以穿孔以允许液体渗透。

公开(公告)号：US20060058520A1

公开(公告)日：2006-03-16

申请号：US11248523

申请日：2005-10-12

申请人： Vern Schramm ,  Jef Boeke ,  Anthony Sauve ,  Ivana Celic

发明人： Vern Schramm ,  Jef Boeke ,  Anthony Sauve ,  Ivana Celic

IPC分类号： C07H19/20

CPC分类号： C12N9/1077 ,  C07H19/04 ,  C07H19/20 ,  C12N9/99 ,  C12P19/32

摘要： A novel compound, 2′/3′-O-acetyl-ADP-ribose, is provided. The compound is a mixture of the 2′ and 3′ regioisomers of O-acetyl-ADP ribose, and is formed nonenzymatically from 2′-O-acetyl-ADP-ribose, which is the newly discovered product of the reaction of Sir2 enzymes with acetylated peptides and NAD+. Analogs of 2′/3′-O-acetyl-ADP-ribose are also provided. Additionally, methods of preparing 2′/3′-O-acetyl-ADP-ribose, methods of determining whether a test compound is an inhibitor of a Sir2 enzyme, methods of detecting Sir2 activity in a composition, methods of deacetylating an acetylated peptide, and methods of inhibiting the deacetylation of an acetylated peptide are provided. Prodrugs of 2′/3′-O-acetyl-ADP-ribose are also provided.

摘要翻译： 提供新的化合物2'/ 3'-O-乙酰基-ADP-核糖。 该化合物是O-乙酰基-ADP核糖的2'和3'区域异构体的混合物，并且由2'-O-乙酰基-ADP-核糖非酶催化形成，其是Sir2酶与 乙酰化肽和NAD +。 还提供了2'/ 3'-O-乙酰基-ADP-核糖的类似物。 另外，制备2'/ 3'-O-乙酰基-ADP-核糖的方法，确定测试化合物是否为Sir2酶抑制剂的方法，组合物中Sir2活性检测方法，乙酰化肽脱乙酰化的方法， 并提供了抑制乙酰化肽脱乙酰化的方法。 还提供了2'/ 3'-O-乙酰基-ADP-核糖的前药。