公开(公告)号：US07279340B2

公开(公告)日：2007-10-09

申请号：US10819831

申请日：2004-04-07

申请人： Victor P. Chu ,  Zhu Teng ,  Steve P. A. Goss ,  Ronald L. Edwards ,  Kelle M. Garvey ,  Timothy J. Gorzynski ,  William D. Bedzyk

发明人： Victor P. Chu ,  Zhu Teng ,  Steve P. A. Goss ,  Ronald L. Edwards ,  Kelle M. Garvey ,  Timothy J. Gorzynski ,  William D. Bedzyk

IPC分类号： G01N33/546 ,  G01N33/543 ,  C07C237/28 ,  G01N37/00

CPC分类号： C07C237/34 ,  C07B2200/11 ,  C07C231/02 ,  Y10S436/815 ,  Y10T436/13

摘要： The present invention relates to immobilized procainamide analogs, as well as to a method of making immobilized procainamide analogs. These immobilized analogs are prepared by activating the carboxyl group on a substituted p-benzoic acid derivative toward nucleophilic attack; reacting the activated benzoic acid derivative with a polyamine to produce the benzoic acid derivative of Formula 3: and binding the benzoic acid derivative of Formula 3 to a latex polymer having functional groups that react with aliphatic amino groups. A method of conducting an immunoassay using the immobilized procainamide analog Formula 3 is described, comprising the steps of preparing a solution comprising said immobilized procainamide analog; adding a sample suspected of containing procainamide to said solution; adding an anti-procainamide antibody to said solution and observing the rate of increase in solution turbidity following antibody addition.

摘要翻译： 本发明涉及固定化的普鲁卡因酰胺类似物，以及制备固定的普鲁卡酰胺类似物的方法。 这些固定化的类似物通过将取代的对苯甲酸衍生物上的羧基活化成亲核攻击来制备; 使活化的苯甲酸衍生物与多胺反应以制备式3的苯甲酸衍生物：将式3的苯甲酸衍生物与具有与脂族氨基反应的官能团的胶乳聚合物结合。 描述了使用固定的普鲁卡酰胺类似物式3进行免疫测定的方法，包括以下步骤：制备包含所述固定的普鲁卡因酰胺类似物的溶液; 在所述溶液中加入怀疑含有普鲁卡因酰胺的样品; 向所述溶液中加入抗普鲁卡因酰胺抗体，并观察抗体加入后溶液浊度的增加速率。

公开(公告)号：US07858399B2

公开(公告)日：2010-12-28

申请号：US11846327

申请日：2007-08-28

申请人： Victor P. Chu ,  Zhu Teng ,  Steve P. A. Goss ,  Ronald A. Edwards ,  Kelle M. Garvey ,  Timothy J. Gorzynski ,  William D. Bedzyk

发明人： Victor P. Chu ,  Zhu Teng ,  Steve P. A. Goss ,  Ronald A. Edwards ,  Kelle M. Garvey ,  Timothy J. Gorzynski ,  William D. Bedzyk

IPC分类号： G01N33/546 ,  G01N33/543 ,  C07C237/28

CPC分类号： C07C237/34 ,  C07B2200/11 ,  C07C231/02 ,  Y10S436/815 ,  Y10T436/13

摘要： The present invention relates to immobilized procainamide analogs, as well as to a method of making immobilized procainamide analogs. These immobilized analogs are prepared by activating the carboxyl group on a substituted p-benzoic acid derivative toward nucleophilic attack; reacting the activated benzoic acid derivative with a polyamine to produce the benzoic acid derivative of Formula 3: and binding the benzoic acid derivative of Formula 3 to a latex polymer having functional groups that react with aliphatic amino groups. A method of conducting an immunoassay using the immobilized procainamide analog Formula 3 is described, comprising the steps of preparing a solution comprising said immobilized procainamide analog; adding a sample suspected of containing procainamide to said solution; adding an anti-procainamide antibody to said solution and observing the rate of increase in solution turbidity following antibody addition.

摘要翻译： 本发明涉及固定化的普鲁卡因酰胺类似物，以及制备固定的普鲁卡酰胺类似物的方法。 这些固定化的类似物通过将取代的对苯甲酸衍生物上的羧基活化成亲核攻击来制备; 使活化的苯甲酸衍生物与多胺反应以制备式3的苯甲酸衍生物：将式3的苯甲酸衍生物与具有与脂族氨基反应的官能团的胶乳聚合物结合。 描述了使用固定的普鲁卡酰胺类似物式3进行免疫测定的方法，包括以下步骤：制备包含所述固定的普鲁卡因酰胺类似物的溶液; 在所述溶液中加入怀疑含有普鲁卡因酰胺的样品; 向所述溶液中加入抗普鲁卡因酰胺抗体，并观察抗体加入后溶液浊度的增加速率。