公开(公告)号：US07279340B2

公开(公告)日：2007-10-09

申请号：US10819831

申请日：2004-04-07

申请人： Victor P. Chu ,  Zhu Teng ,  Steve P. A. Goss ,  Ronald L. Edwards ,  Kelle M. Garvey ,  Timothy J. Gorzynski ,  William D. Bedzyk

发明人： Victor P. Chu ,  Zhu Teng ,  Steve P. A. Goss ,  Ronald L. Edwards ,  Kelle M. Garvey ,  Timothy J. Gorzynski ,  William D. Bedzyk

IPC分类号： G01N33/546 ,  G01N33/543 ,  C07C237/28 ,  G01N37/00

CPC分类号： C07C237/34 ,  C07B2200/11 ,  C07C231/02 ,  Y10S436/815 ,  Y10T436/13

摘要： The present invention relates to immobilized procainamide analogs, as well as to a method of making immobilized procainamide analogs. These immobilized analogs are prepared by activating the carboxyl group on a substituted p-benzoic acid derivative toward nucleophilic attack; reacting the activated benzoic acid derivative with a polyamine to produce the benzoic acid derivative of Formula 3: and binding the benzoic acid derivative of Formula 3 to a latex polymer having functional groups that react with aliphatic amino groups. A method of conducting an immunoassay using the immobilized procainamide analog Formula 3 is described, comprising the steps of preparing a solution comprising said immobilized procainamide analog; adding a sample suspected of containing procainamide to said solution; adding an anti-procainamide antibody to said solution and observing the rate of increase in solution turbidity following antibody addition.

摘要翻译： 本发明涉及固定化的普鲁卡因酰胺类似物，以及制备固定的普鲁卡酰胺类似物的方法。 这些固定化的类似物通过将取代的对苯甲酸衍生物上的羧基活化成亲核攻击来制备; 使活化的苯甲酸衍生物与多胺反应以制备式3的苯甲酸衍生物：将式3的苯甲酸衍生物与具有与脂族氨基反应的官能团的胶乳聚合物结合。 描述了使用固定的普鲁卡酰胺类似物式3进行免疫测定的方法，包括以下步骤：制备包含所述固定的普鲁卡因酰胺类似物的溶液; 在所述溶液中加入怀疑含有普鲁卡因酰胺的样品; 向所述溶液中加入抗普鲁卡因酰胺抗体，并观察抗体加入后溶液浊度的增加速率。

公开(公告)号：US07858399B2

公开(公告)日：2010-12-28

申请号：US11846327

申请日：2007-08-28

申请人： Victor P. Chu ,  Zhu Teng ,  Steve P. A. Goss ,  Ronald A. Edwards ,  Kelle M. Garvey ,  Timothy J. Gorzynski ,  William D. Bedzyk

发明人： Victor P. Chu ,  Zhu Teng ,  Steve P. A. Goss ,  Ronald A. Edwards ,  Kelle M. Garvey ,  Timothy J. Gorzynski ,  William D. Bedzyk

IPC分类号： G01N33/546 ,  G01N33/543 ,  C07C237/28

CPC分类号： C07C237/34 ,  C07B2200/11 ,  C07C231/02 ,  Y10S436/815 ,  Y10T436/13

摘要： The present invention relates to immobilized procainamide analogs, as well as to a method of making immobilized procainamide analogs. These immobilized analogs are prepared by activating the carboxyl group on a substituted p-benzoic acid derivative toward nucleophilic attack; reacting the activated benzoic acid derivative with a polyamine to produce the benzoic acid derivative of Formula 3: and binding the benzoic acid derivative of Formula 3 to a latex polymer having functional groups that react with aliphatic amino groups. A method of conducting an immunoassay using the immobilized procainamide analog Formula 3 is described, comprising the steps of preparing a solution comprising said immobilized procainamide analog; adding a sample suspected of containing procainamide to said solution; adding an anti-procainamide antibody to said solution and observing the rate of increase in solution turbidity following antibody addition.

摘要翻译： 本发明涉及固定化的普鲁卡因酰胺类似物，以及制备固定的普鲁卡酰胺类似物的方法。 这些固定化的类似物通过将取代的对苯甲酸衍生物上的羧基活化成亲核攻击来制备; 使活化的苯甲酸衍生物与多胺反应以制备式3的苯甲酸衍生物：将式3的苯甲酸衍生物与具有与脂族氨基反应的官能团的胶乳聚合物结合。 描述了使用固定的普鲁卡酰胺类似物式3进行免疫测定的方法，包括以下步骤：制备包含所述固定的普鲁卡因酰胺类似物的溶液; 在所述溶液中加入怀疑含有普鲁卡因酰胺的样品; 向所述溶液中加入抗普鲁卡因酰胺抗体，并观察抗体加入后溶液浊度的增加速率。

公开(公告)号：US07161034B2

公开(公告)日：2007-01-09

申请号：US10828601

申请日：2004-04-20

申请人： Victor P. Chu ,  Zhu Teng ,  Sandra Lewisch ,  Ronald L. Edwards

发明人： Victor P. Chu ,  Zhu Teng ,  Sandra Lewisch ,  Ronald L. Edwards

IPC分类号： C07C233/05 ,  A61K31/165

CPC分类号： C07C237/04 ,  C07C275/40 ,  G01N33/54313 ,  Y02P20/55 ,  Y10S436/811 ,  Y10S436/815

摘要： Lidocaine analogs, latex particle-analog conjugates, and methods of making and using the same. The analogs have the formula: wherein Z comprises a nucleophilic group and optionally a protecting group; L is a linker; R1, R2, R3, R4, R5 are each independently H, a protecting group, or C1 to C6 alkyls, provided that R1 and R3 may form a six membered ring with the nitrogen and carbon atom to which R1 and R3 are attached; R6 and R7 are each independently H or C1 to C20 alkyls; and including salts thereof. The analogs are immobilized on latex particles and prepared for use in assays for lidocaine.

摘要翻译： 利多卡因类似物，胶乳颗粒 - 类似物共轭物，以及制备和使用它们的方法。 类似物具有下式：其中Z包括亲核基团和任选的保护基团; L是连接体; R 1，R 2，R 3，R 4，R 5，...， 各自独立地为H，保护基，或C 1至C 6烷基，条件是R 1和R 3 >可以与R 1和R 3连接的氮原子和碳原子形成六元环; R 6和R 7各自独立地为H或C 1至C 20烷基; 并包括其盐。 类似物固定在胶乳颗粒上并准备用于利多卡因的测定。