公开(公告)号：US07279340B2

公开(公告)日：2007-10-09

申请号：US10819831

申请日：2004-04-07

申请人： Victor P. Chu ,  Zhu Teng ,  Steve P. A. Goss ,  Ronald L. Edwards ,  Kelle M. Garvey ,  Timothy J. Gorzynski ,  William D. Bedzyk

发明人： Victor P. Chu ,  Zhu Teng ,  Steve P. A. Goss ,  Ronald L. Edwards ,  Kelle M. Garvey ,  Timothy J. Gorzynski ,  William D. Bedzyk

IPC分类号： G01N33/546 ,  G01N33/543 ,  C07C237/28 ,  G01N37/00

CPC分类号： C07C237/34 ,  C07B2200/11 ,  C07C231/02 ,  Y10S436/815 ,  Y10T436/13

摘要： The present invention relates to immobilized procainamide analogs, as well as to a method of making immobilized procainamide analogs. These immobilized analogs are prepared by activating the carboxyl group on a substituted p-benzoic acid derivative toward nucleophilic attack; reacting the activated benzoic acid derivative with a polyamine to produce the benzoic acid derivative of Formula 3: and binding the benzoic acid derivative of Formula 3 to a latex polymer having functional groups that react with aliphatic amino groups. A method of conducting an immunoassay using the immobilized procainamide analog Formula 3 is described, comprising the steps of preparing a solution comprising said immobilized procainamide analog; adding a sample suspected of containing procainamide to said solution; adding an anti-procainamide antibody to said solution and observing the rate of increase in solution turbidity following antibody addition.

摘要翻译： 本发明涉及固定化的普鲁卡因酰胺类似物，以及制备固定的普鲁卡酰胺类似物的方法。 这些固定化的类似物通过将取代的对苯甲酸衍生物上的羧基活化成亲核攻击来制备; 使活化的苯甲酸衍生物与多胺反应以制备式3的苯甲酸衍生物：将式3的苯甲酸衍生物与具有与脂族氨基反应的官能团的胶乳聚合物结合。 描述了使用固定的普鲁卡酰胺类似物式3进行免疫测定的方法，包括以下步骤：制备包含所述固定的普鲁卡因酰胺类似物的溶液; 在所述溶液中加入怀疑含有普鲁卡因酰胺的样品; 向所述溶液中加入抗普鲁卡因酰胺抗体，并观察抗体加入后溶液浊度的增加速率。

公开(公告)号：US07858399B2

公开(公告)日：2010-12-28

申请号：US11846327

申请日：2007-08-28

申请人： Victor P. Chu ,  Zhu Teng ,  Steve P. A. Goss ,  Ronald A. Edwards ,  Kelle M. Garvey ,  Timothy J. Gorzynski ,  William D. Bedzyk

发明人： Victor P. Chu ,  Zhu Teng ,  Steve P. A. Goss ,  Ronald A. Edwards ,  Kelle M. Garvey ,  Timothy J. Gorzynski ,  William D. Bedzyk

IPC分类号： G01N33/546 ,  G01N33/543 ,  C07C237/28

CPC分类号： C07C237/34 ,  C07B2200/11 ,  C07C231/02 ,  Y10S436/815 ,  Y10T436/13

摘要： The present invention relates to immobilized procainamide analogs, as well as to a method of making immobilized procainamide analogs. These immobilized analogs are prepared by activating the carboxyl group on a substituted p-benzoic acid derivative toward nucleophilic attack; reacting the activated benzoic acid derivative with a polyamine to produce the benzoic acid derivative of Formula 3: and binding the benzoic acid derivative of Formula 3 to a latex polymer having functional groups that react with aliphatic amino groups. A method of conducting an immunoassay using the immobilized procainamide analog Formula 3 is described, comprising the steps of preparing a solution comprising said immobilized procainamide analog; adding a sample suspected of containing procainamide to said solution; adding an anti-procainamide antibody to said solution and observing the rate of increase in solution turbidity following antibody addition.

摘要翻译： 本发明涉及固定化的普鲁卡因酰胺类似物，以及制备固定的普鲁卡酰胺类似物的方法。 这些固定化的类似物通过将取代的对苯甲酸衍生物上的羧基活化成亲核攻击来制备; 使活化的苯甲酸衍生物与多胺反应以制备式3的苯甲酸衍生物：将式3的苯甲酸衍生物与具有与脂族氨基反应的官能团的胶乳聚合物结合。 描述了使用固定的普鲁卡酰胺类似物式3进行免疫测定的方法，包括以下步骤：制备包含所述固定的普鲁卡因酰胺类似物的溶液; 在所述溶液中加入怀疑含有普鲁卡因酰胺的样品; 向所述溶液中加入抗普鲁卡因酰胺抗体，并观察抗体加入后溶液浊度的增加速率。

公开(公告)号：US10088490B2

公开(公告)日：2018-10-02

申请号：US12860600

申请日：2010-08-20

申请人： Sandra A. Lewisch ,  Lynn M. Schiavoni ,  William D. Bedzyk

发明人： Sandra A. Lewisch ,  Lynn M. Schiavoni ,  William D. Bedzyk

IPC分类号： G01N33/74 ,  G01N33/536 ,  G01N33/53 ,  G01N33/94

摘要： A method for determining an analyte in a sample suspected of containing the analyte comprises providing in combination a medium, the sample, and two or more different receptors. Each different receptor binds to at least two different epitopic sites. One of the epitopic sites is a common binding site and one of the epitopic sites is non-common binding site. The non-common epitopic sites are different for each different receptor. The receptors exhibit mono-molecular binding. The medium is incubated under conditions for binding of the receptors to the epitopic sites. The medium is examined for the presence and/or amount of complexes comprising the epitopic sites and the receptors. The presence and/or amount of the complexes indicate the presence and/or amount of the analyte in the sample.

公开(公告)号：US20120045847A1

公开(公告)日：2012-02-23

申请号：US12860600

申请日：2010-08-20

申请人： Sandra A. Lewisch ,  Lynn M. Schiavoni ,  William D. Bedzyk

发明人： Sandra A. Lewisch ,  Lynn M. Schiavoni ,  William D. Bedzyk

IPC分类号： G01N33/566

CPC分类号： G01N33/743 ,  G01N33/5306 ,  G01N33/536 ,  G01N33/9493

摘要： A method for determining an analyte in a sample suspected of containing the analyte comprises providing in combination a medium, the sample, and two or more different receptors. Each different receptor binds to at least two different epitopic sites. One of the epitopic sites is a common binding site and one of the epitopic sites is non-common binding site. The non-common epitopic sites are different for each different receptor. The receptors exhibit mono-molecular binding. The medium is incubated under conditions for binding of the receptors to the epitopic sites. The medium is examined for the presence and/or amount of complexes comprising the epitopic sites and the receptors. The presence and/or amount of the complexes indicate the presence and/or amount of the analyte in the sample.

摘要翻译： 用于测定怀疑含有分析物的样品中的分析物的方法包括组合提供培养基，样品和两种或更多种不同的受体。 每个不同的受体结合至少两个不同的表位位点。 其中一个表位位点是常见的结合位点，其中一个表位位点是非常见的结合位点。 非常见的表位位点对于每种不同的受体是不同的。 受体表现出单分子结合。 将培养基在受体与表位位点结合的条件下孵育。 检查培养基中包含表位位点和受体的复合物的存在和/或量。 复合物的存在和/或量表示样品中分析物的存在和/或量。