公开(公告)号：US08388960B2

公开(公告)日：2013-03-05

申请号：US13229422

申请日：2011-09-09

申请人： Viktor S. Goldmakher ,  Robert J. Lutz ,  Ravi V. J. Chari ,  Yelena V. Kovtun ,  John M. Lambert ,  Rita Steeves ,  Hans K. Erickson ,  Wayne C. Widdison

发明人： Viktor S. Goldmakher ,  Robert J. Lutz ,  Ravi V. J. Chari ,  Yelena V. Kovtun ,  John M. Lambert ,  Rita Steeves ,  Hans K. Erickson ,  Wayne C. Widdison

IPC分类号： A61K39/00

CPC分类号： C07K16/3046 ,  A61K31/5383 ,  A61K31/58 ,  A61K47/6803 ,  A61K47/6849 ,  A61K2039/505 ,  C07D498/18 ,  C07K2317/24

摘要： Methods of killing or inhibiting tumors comprising of heterogeneous or mixed cell populations is described. The killing or inhibition of tumors is achieved by selectively targeting a unique ligand suspected of being expressed on a particular cell population to also kill a cell population lacking the unique ligand. These conjugates have therapeutic use as they are delivered to a specific cell population to kill these cells and the cytotoxic drug is released to kill non-targeted cells, thereby eliminating the tumor.

摘要翻译： 描述了杀死或抑制包含异源或混合细胞群的肿瘤的方法。 通过选择性地靶向疑似在特定细胞群体上表达的独特配体也杀死缺乏独特配体的细胞群体来实现肿瘤的杀伤或抑制。 这些缀合物具有治疗用途，因为它们被递送到特定细胞群以杀死这些细胞，并释放细胞毒性药物以杀死非靶向细胞，从而消除肿瘤。

公开(公告)号：US08137669B2

公开(公告)日：2012-03-20

申请号：US12186411

申请日：2008-08-05

申请人： Viktor S. Goldmakher ,  Robert J. Lutz ,  Ravi V. J. Chari ,  Yelena V. Kovtun ,  John M. Lambert ,  Rita Steeves ,  Hans K. Erickson

发明人： Viktor S. Goldmakher ,  Robert J. Lutz ,  Ravi V. J. Chari ,  Yelena V. Kovtun ,  John M. Lambert ,  Rita Steeves ,  Hans K. Erickson

IPC分类号： A61K39/00

CPC分类号： C07K16/3046 ,  A61K31/5383 ,  A61K31/58 ,  A61K47/6803 ,  A61K47/6849 ,  A61K2039/505 ,  C07D498/18 ,  C07K2317/24

摘要： Methods of killing or inhibiting tumors comprising of heterogeneous or mixed cell populations is described. The killing or inhibition of tumors is achieved by selectively targeting a unique ligand suspected of being expressed on a particular cell population to also kill a cell population lacking the unique ligand. These conjugates have therapeutic use as they are delivered to a specific cell population to kill these cells and the cytotoxic drug is released to kill non-targeted cells, thereby eliminating the tumor.

摘要翻译： 描述了杀死或抑制包含异源或混合细胞群的肿瘤的方法。 通过选择性地靶向疑似在特定细胞群体上表达的独特配体也杀死缺乏独特配体的细胞群体来实现肿瘤的杀伤或抑制。 这些缀合物具有治疗用途，因为它们被递送到特定细胞群以杀死这些细胞，并释放细胞毒性药物以杀死非靶向细胞，从而消除肿瘤。

公开(公告)号：US20090076263A1

公开(公告)日：2009-03-19

申请号：US12186411

申请日：2008-08-05

申请人： Viktor S. Goldmakher ,  Robert J. Lutz ,  Ravi V.J. Chari ,  Yelena V. Kovtun ,  John M. Lambert ,  Rita Steeves ,  Hans K. Erickson

发明人： Viktor S. Goldmakher ,  Robert J. Lutz ,  Ravi V.J. Chari ,  Yelena V. Kovtun ,  John M. Lambert ,  Rita Steeves ,  Hans K. Erickson

IPC分类号： C07D498/12

CPC分类号： C07K16/3046 ,  A61K31/5383 ,  A61K31/58 ,  A61K47/6803 ,  A61K47/6849 ,  A61K2039/505 ,  C07D498/18 ,  C07K2317/24

摘要： Methods of killing or inhibiting tumors comprising of heterogeneous or mixed cell populations is described. The killing or inhibition of tumors is achieved by selectively targeting a unique ligand suspected of being expressed on a particular cell population to also kill a cell population lacking the unique ligand. These conjugates have therapeutic use as they are delivered to a specific cell population to kill these cells and the cytotoxic drug is released to kill non-targeted cells, thereby eliminating the tumor.

摘要翻译： 描述了杀死或抑制包含异源或混合细胞群的肿瘤的方法。 通过选择性地靶向疑似在特定细胞群体上表达的独特配体也杀死缺乏独特配体的细胞群体来实现肿瘤的杀伤或抑制。 这些缀合物具有治疗用途，因为它们被递送到特定细胞群以杀死这些细胞，并释放细胞毒性药物以杀死非靶向细胞，从而消除肿瘤。

公开(公告)号：US06613959B1

公开(公告)日：2003-09-02

申请号：US09371338

申请日：1999-08-10

申请人： Jen Sheen ,  Yelena V. Kovtun ,  Wan-Ling Chiu

发明人： Jen Sheen ,  Yelena V. Kovtun ,  Wan-Ling Chiu

IPC分类号： C12N1509

CPC分类号： C12N9/1205 ,  C12N9/16 ,  C12N15/8266 ,  C12N15/827 ,  C12N15/8271 ,  C12N15/8287 ,  C12N15/8294

摘要： The invention features a method for increasing stress resistance or tolerance in a plant, the method including the steps of: (a) introducing into plant cells a transgene including DNA encoding a kinase domain of a mitogen-activated protein kinase kinase kinase (MAPKKK) operably linked to a promoter functional in plant cells to yield transformed plant cells; and (b) regenerating a transgenic plant from the transformed cells, wherein the kinase domain of said MAPKKK is expressed in the cells of the transgenic plant, thereby increasing the level of stress resistance or tolerance in the transgenic plant. The invention further features plants including a recombinant transgene capable of expressing a kinase domain of a mitogen-activated protein kinase kinase kinase (MAPKKK) or a kinase domain thereof, wherein the transgene is expressed in said plant under the control of a promoter that is functional in a plant cell.

摘要翻译： 本发明的特征在于增加植物的胁迫抗性或耐受性的方法，所述方法包括以下步骤：（a）可操作地向植物细胞中引入包含编码丝裂原活化蛋白激酶激酶激酶（MAPKKK）的激酶结构域的DNA的转基因 与在植物细胞中起作用的启动子连接以产生转化的植物细胞; 和（b）从转化的细胞再生转基因植物，其中所述MAPKKK的激酶结构域在转基因植物的细胞中表达，由此增加转基因植物的胁迫抗性或耐受性水平。 本发明还涉及包括能够表达丝裂原活化蛋白激酶激酶激酶（MAPKKK）或其激酶结构域的激酶结构域的重组转基因的植物，其中所述植物在所述植物中表达，所述启动子是功能性的 在植物细胞中。