公开(公告)号：US11673913B2

公开(公告)日：2023-06-13

申请号：US16408711

申请日：2019-05-10

Applicant: Virginia Commonwealth University

Inventor： Heather R. Lucas ,  Ricardo D. Fernandez

IPC: C07K1/30 ,  C07K14/47 ,  C07K1/36 ,  G01N33/68 ,  C12N15/70

CPC classification number: C07K1/303 ,  C07K1/36 ,  C07K14/47 ,  C12N15/70 ,  G01N33/68 ,  G01N2800/2835

Abstract: Tetrameric N-terminally acetylated α-synuclein is prepared by transforming an expression system with an expression vector encoding α-synuclein, wherein the expression system expresses a native NatB acetylase complex or ortholog thereof and/or wherein an exogenous NatB acetylase complex or ortholog thereof is co-expressed in the expression system, inducing protein expression in the transformed expression system, lysing cells in the transformed expression system to produce a cell lysate, performing salt precipitation of the cell lysate, recovering tetrameric N-terminally acetylated α-synuclein by centrifugation, and purifying the tetrameric N-terminally acetylated α-synuclein. Compositions comprising the same and methods for identifying compounds that stabilize natively folded tetrameric α-synuclein are also provided.