-
公开(公告)号：US09988419B2
公开(公告)日：2018-06-05
申请号：US14766131
申请日：2014-02-05
发明人： Peter Stanley Gagnon
摘要： A method for the purification of a desired protein from a protein preparation includes conditioning the protein preparation by treatment with soluble organic multivalent ions, immobilized organic multivalent ions, or both, optionally in the presence of supersaturated allantoin, thereby removing at least 90% of chromatin, then (1) precipitating the desired protein with a nonionic organic polymer in the presence of non-protein-precipitating salts at greater than physiological concentration to provide a precipitate of the desired protein; or (2) precipitating the desired protein with a nonionic organic polymer in the absence of non-precipitating salts at greater than physiological concentration to provide a precipitate and subsequently washing the precipitate with a nonionic organic polymer in the presence of non-protein-precipitating salts at greater than physiological concentration.
-
2.发明授权公开(公告)号：US09643998B2
公开(公告)日：2017-05-09
申请号：US13315376
申请日：2011-12-09
CPC分类号： C07K1/30 , C07K1/24 , C07K1/303 , C07K1/34 , G01N27/021 , G01R27/00 , Y10T436/115831 , Y10T436/12 , Y10T436/25375
摘要： Methods and systems for monitoring and/or controlling protein separation, purification, extraction, and/or fractionation processes are provided. The impedance of a protein mixture undergoing a protein process is measured and compared to a target reference impedance value or range of reference impedance values. If the measured impedance is not within an acceptable deviation of the target reference impedance value, a parameter of the protein mixture or process is adjusted.
-
公开(公告)号：US20140329991A9
公开(公告)日：2014-11-06
申请号：US13744305
申请日：2013-01-17
IPC分类号： C07K1/30
CPC分类号： C07K1/303
摘要： The invention provides methods and apparatus for the selective isolation of phosphorylated and glycosylated proteins and their fragments. A lanthanide metal cation is used to precipitate proteins or protein fragments containing phospho groups and/or glyco groups. The sample preparation method can be used for many types of biological samples, including HeLa cells, food, and human cerebrospinal fluid. The proteins are isolated, recovered and ready for analysis by mass spectrometry or other analytical methods allowing detection limits down to the femtomole level. The method and apparatus are valuable tools in the field of protein analysis and diagnostics.
-
公开(公告)号：US20110092671A1
公开(公告)日：2011-04-21
申请号：US12999606
申请日：2008-06-17
CPC分类号： C07K1/303 , C07K5/0817 , C07K5/0821 , C07K7/06 , C07K14/4723
摘要： A process for the manufacture of peptide Omiganan in solution phase. Novel intermediates in the process for the manufacture of Omiganan and processes for the manufacture of these intermediates.
摘要翻译： 在溶液相中制造肽Omiganan的方法。 在制造Omiganan过程中的新型中间体以及制造这些中间体的方法。
-
公开(公告)号：US20100047428A1
公开(公告)日：2010-02-25
申请号：US12300495
申请日：2007-05-31
申请人： Alain Lejars , Michel Nogre , Michael Tellier
发明人： Alain Lejars , Michel Nogre , Michael Tellier
IPC分类号： A23C7/04 , C07K14/745 , A23C9/20
CPC分类号： C07K14/745 , C07K1/303 , C07K1/36 , C12N9/644 , C12Y304/21022
摘要： The invention is related to a process for extracting at least one protein present in milk, said protein exhibiting an affinity for the complexed or non-complexed calcium ions of said milk, comprising the following steps consisting of: a) releasing the protein by precipitation of calcium compounds obtained by contacting the milk with a soluble salt, the anion of which is selected for its capability to form said insoluble calcium compounds in such a medium, in order to obtain in this way a protein-enriched liquid phase, b) separating the protein-enriched liquid phase from the precipitate of calcium compounds, said liquid phase being, moreover, separated in a lipidic phase and in a non-lipidic aqueous phase comprising the protein, and c) recovering the non-lipidic aqueous phase comprising the protein.
摘要翻译： 本发明涉及一种用于提取乳中存在的至少一种蛋白质的方法，所述蛋白质对所述乳的络合或非络合钙离子表现出亲和力，包括以下步骤：a）通过沉淀来释放蛋白质 通过使牛奶与可溶性盐接触获得的钙化合物，其可选择的阴离子是为了在这种介质中形成所述不溶性钙化合物的能力，以便以这种方式获得富含蛋白质的液相，b）将 此外，在脂质相和包含蛋白质的非脂质水相中分离所述液相，和c）回收包含蛋白质的非脂质水相。
-
公开(公告)号：US4322275A
公开(公告)日：1982-03-30
申请号：US197394
申请日：1980-10-16
申请人： Surendar M. Jain
发明人： Surendar M. Jain
IPC分类号： A23C9/144 , A23C21/00 , A23J1/00 , A23J1/06 , A23J1/20 , A61K35/14 , A61K38/00 , A61K38/16 , A61K39/395 , A61M1/22 , A61M1/34 , A61M1/36 , B01D61/44 , B01D61/54 , B04B5/10 , C07K1/14 , C07K1/30 , C07K1/34 , C07K1/36 , C07K14/00 , C07K14/435 , C07K14/745 , C07K14/76 , C07K14/765 , C07K16/00 , C07K16/06 , B01D13/02 , A61K37/04
CPC分类号： B01D61/44 , A23C9/144 , A23J1/00 , A23J1/06 , A61M1/3482 , A61M1/3486 , B01D61/422 , C07K1/303 , C07K1/36 , C07K14/745 , C07K14/765 , C07K16/065 , A61K38/00 , B01D2311/18 , B01D2311/2642 , Y10S128/22 , Y10S436/824 , Y10S530/83 , Y10S530/833
摘要： This invention relates to the separation of complex protein mixtures by lowering (desalting) or by raising (salting-out) their electrolytic (ionic) concentration by electrodialysis followed by chilling, pH adjustment, filtration, and/or centrifugation and optionally thereafter restoration of the lost electrolyte or removal of the gained electrolyte and water. This technique is especially adaptable for therapeutic plasma exchange performed in situ for immunepheresis i.e. removal of globulin or immunecomplexes implicated in autoimmune diseases. The techniques are also applicable for antihemophilic factor separation and purification, and separation of proteins from whey.
摘要翻译： 本发明涉及通过降低（脱盐）或通过电渗析升高（盐析）其电解（离子）浓度，随后进行冷却，pH调节，过滤和/或离心，并任选地将 失去电解质或去除所获得的电解液和水。 该技术特别适用于用于免疫测定法原位进行的治疗性血浆置换，即去除与自身免疫性疾病有关的球蛋白或免疫复合物。 该技术也适用于抗血友病因子分离和纯化，以及蛋白质与乳清的分离。
-
公开(公告)号：US09409946B2
公开(公告)日：2016-08-09
申请号：US12999606
申请日：2008-06-17
CPC分类号： C07K1/303 , C07K5/0817 , C07K5/0821 , C07K7/06 , C07K14/4723
摘要： A process for the manufacture of peptide Omiganan in solution phase. Novel intermediates in the process for the manufacture of Omiganan and processes for the manufacture of these intermediates.
摘要翻译： 在溶液相中制造肽Omiganan的方法。 在制造Omiganan过程中的新型中间体以及制造这些中间体的方法。
-
公开(公告)号：US08574883B2
公开(公告)日：2013-11-05
申请号：US13744421
申请日：2013-01-18
CPC分类号： C07K1/303 , C07K1/30 , C07K14/77 , C07K14/79 , C07K14/8135 , C12N9/2462 , C12Y302/01017
摘要： A method of co-extracting multiple proteins from chicken egg white is provided. Precipitate proteins step-by-step with polyethylene glycol, then separate the proteins via Q Sepharose Fast Flow anion-exchange chromatography. The method of co-extracting multiple proteins from chicken egg white is capable of obtaining five proteins simultaneously in one extraction process, and the protein products have high purity and high recovery ratio. And the method can be carried out only requiring common-used chromatographic filler, greatly reduces production cost, and provides favorable factors for large-scale industrial production of extracting protein from egg white.
-
9.发明申请Real Time Monitoring and Control of Protein Production Processes Using Impedance Spectroscopy 有权使用阻抗光谱法实时监测和控制蛋白质生产过程公开(公告)号：US20120153221A1
公开(公告)日：2012-06-21
申请号：US13315376
申请日：2011-12-09
CPC分类号： C07K1/30 , C07K1/24 , C07K1/303 , C07K1/34 , G01N27/021 , G01R27/00 , Y10T436/115831 , Y10T436/12 , Y10T436/25375
摘要： Methods and systems for monitoring and/or controlling protein separation, purification, extraction, and/or fractionation processes are provided. The impedance of a protein mixture undergoing a protein process is measured and compared to a target reference impedance value or range of reference impedance values. If the measured impedance is not within an acceptable deviation of the target reference impedance value, a parameter of the protein mixture or process is adjusted.
摘要翻译： 提供了用于监测和/或控制蛋白质分离，纯化，提取和/或分馏过程的方法和系统。 测量经历蛋白质过程的蛋白质混合物的阻抗并将其与目标参考阻抗值或参考阻抗值的范围进行比较。 如果测量的阻抗不在目标参考阻抗值的可接受偏差范围内，则调整蛋白质混合物或过程的参数。
-
公开(公告)号：US20090286967A1
公开(公告)日：2009-11-19
申请号：US12383457
申请日：2009-03-23
申请人： Jeffrey Kitzler , Abdul Ibrahim
发明人： Jeffrey Kitzler , Abdul Ibrahim
CPC分类号： C07K1/303
摘要： A method and kit for precipitating protein from an aqueous solution consists of a quaternary ammonium cationic surfactant and a chaotropic salt, used in combination to reversibly precipitate proteins from the aqueous solution. Precipitation separates proteins that are entrapped, non-specifically, in the quaternary ammonium cationic surfactant:chaotropic salt complex as a pellet. The precipitated protein pellet is purified further by washing with an organic solvent to remove the surfactant:chaotrope complex, leaving behind pure, concentrated proteins that can be washed to purify further the protein and/or re-dissolved in whatever buffer is most suited for any successive procedure, such as analysis.
摘要翻译： 用于从水溶液中沉淀蛋白质的方法和试剂盒由季铵阳离子表面活性剂和离液盐组成，用于从水溶液中可逆地沉淀蛋白质。 沉淀分离在季铵阳离子表面活性剂中非截留的蛋白质：离液盐络合物作为颗粒。 通过用有机溶剂洗涤进一步纯化沉淀的蛋白质沉淀物以除去表面活性剂：离液混合物，留下纯的浓缩蛋白质，其可以进一步洗涤以纯化蛋白质和/或重新溶解在任何最适合于任何 连续的程序，如分析。
-
-
-
-
-
-
-
-
-
搜索结果
40 条记录 (耗时 0.019 秒)