公开(公告)号：US20220299500A1

公开(公告)日：2022-09-22

申请号：US17597995

申请日：2020-09-30

Applicant: Virginia Tech Intellectual Properties Inc.

Inventor： Aloka B. BANDARA ,  David A. BROWN

IPC: G01N33/50 ,  C12N9/02 ,  C12N15/11 ,  C12N9/22

Abstract: Disclosed herein is a recombinant human cell line that does not express functional endogenous NADH dehydrogenase [ubiquinone] iron-sulfur protein 2 (NDUFS2). This knockout cell line can be used to advance the basic understanding of the NDUFS2 protein subunit in overall complex I assembly and function. Furthermore, this cell line can be used to test therapeutic agents to fix or bypass the dysfunctional complex I.