公开(公告)号：US20160136544A1

公开(公告)日：2016-05-19

申请号：US14898014

申请日：2014-06-12

申请人： WATERS TECHNOLOGIES CORPORATION

发明人： Christopher Hudalla

IPC分类号： B01D15/40 ,  G01N30/32 ,  B01D15/16

CPC分类号： B01D15/40 ,  B01D15/16 ,  B01D15/163 ,  B01J3/02 ,  G01N30/32 ,  G01N30/8658 ,  G01N2030/326

摘要： A methodology scales supercritical fluid chromatography (SFC) and/or carbon dioxide based chromatography methods between different system and/or column configurations. The methodology includes measuring an average mobile phase density during a first separation utilizing CO2 as a mo bile phase component and substantially duplicating the average density profile for a second separation. Substantial duplication of the average mobile phase density (e.g., within about 10%, 5%, 2.5%, 1%, 0.5%, 0.1 %, 0.05%) results in chromatography for both system and/or column configurations having similar selectivity and retention factors. Average mobile phase density may be, either measured directly, calculated, or approximated using average pressure or density measurements. The average pressure profile may be used as a close approximation to duplicate average density profiles between separations.

摘要翻译： A方法在不同系统和/或柱构型之间进行超临界流体色谱（SFC）和/或基于二氧化碳的色谱法的比较。 该方法包括在利用CO 2作为微量相分量的第一分离期间测量平均流动相密度，并基本上复制用于第二分离的平均密度分布。 平均流动相密度的显着重复（例如，在约10％，5％，2.5％，1％，0.5％，0.1％，0.05％之内）导致具有相似选择性和保留性的系统和/或柱构型的色谱法 因素 可以使用平均压力或密度测量直接测量，计算或近似平均流动相密度。 平均压力分布可以用于近似分离之间的重复平均密度分布。