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公开(公告)号：US20240361318A1
公开(公告)日：2024-10-31
申请号：US18647863
申请日：2024-04-26
IPC分类号： G01N33/569 , G01N33/53 , G01N33/543 , G01N33/74
CPC分类号： G01N33/56983 , G01N33/5308 , G01N33/54313 , G01N33/54386 , G01N33/74 , G01N2333/015 , G01N2333/62 , G01N2469/10
摘要： The present technology is directed to methods of preparing affinity chromatographic columns using streptavidin-conjugated particles and biotinylated affinity agents (i.e., biotinylated oligonucleotides, biotinylated antibodies or biotinylated antigen-binding fragments thereof). The methods of the present technology can be utilized to perform affinity capture assays in a high-throughput, efficient manner. Further, the affinity chromatographic columns disclosed herein are customizable due to the ability to utilize any biotinylated affinity agent. The methods herein utilize an on-line loading (i.e., loading the column with biotinylated affinity agent via a liquid chromatography system).
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公开(公告)号：US12121829B2
公开(公告)日：2024-10-22
申请号：US16800928
申请日：2020-02-25
CPC分类号： B01D15/22 , C08J7/065 , G01N30/32 , G01N30/6026 , G01N30/603 , G01N30/6052 , G01N2030/027 , G01N2030/324
摘要： The present disclosure relates to a filter. The filter includes a porous element, a compression element and a housing. At least a portion of the porous element is coated with an alkylsilyl coating. The compression element is configured to receive the porous element thereby forming an assembly. The housing has an opening formed therein. The opening is configured to receive the assembly. The assembly is retained within the opening when the assembly is received therein.
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3.发明公开公开(公告)号：US20240335830A1
公开(公告)日：2024-10-10
申请号：US18749930
申请日：2024-06-21
CPC分类号： B01L3/5023 , B01D15/3809 , B01D15/424 , B01L2200/0631 , B01L2200/0668 , B01L2300/0681 , B01L2300/069 , B01L2400/0409 , B01L2400/0433
摘要： A sample preparation device is provided for enriching a component of a sample. The sample preparation device includes a surface in fluid communication with the sample, an alkylsilyl coating disposed on the surface, and an affinity ligand or an enzyme covalently bonded to the alkylsilyl coating.
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公开(公告)号：US20240302334A1
公开(公告)日：2024-09-12
申请号：US18599970
申请日：2024-03-08
发明人： Jinchuan Yang , Paul Rainville
CPC分类号： G01N30/56 , G01N30/06 , G01N30/7233 , G01N30/74 , G01N2030/045 , G01N2030/567
摘要： The present disclosure is directed to methods of separating one or more non-modified oligosaccharide(s) with degree of polymerization 6 or higher from a sample using a hydrophilic interaction liquid chromatography (HILIC) column connected to a liquid chromatography system that includes coated flow paths. The methods disclosed herein requires, inter alia, a liquid chromatography system, wherein at least one component of the LC system includes a fluid-contacting alkylsilyl coating. When implementing a standard HILIC method, the methods of the present technology can be used to decrease the analyte loss and/or carry-over of the separation leading to improved analysis.
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公开(公告)号：US12083498B2
公开(公告)日：2024-09-10
申请号：US16926102
申请日：2020-07-10
发明人： Darryl W. Brousmiche , Jacob N. Fairchild , Jason F. Hill , Giorgis Isaac , Michael F. Morris , Kevin D. Wyndham
IPC分类号： B01D15/26 , B01D15/30 , B01D15/32 , B01D15/40 , B01D53/02 , B01J20/22 , B01J20/28 , B01J20/282 , B01J20/283 , B01J20/284 , B01J20/285 , B01J20/288 , B01J20/289 , B01J20/30 , B01J20/32 , C07F7/18
CPC分类号： B01J20/283 , B01D15/26 , B01D15/30 , B01D15/305 , B01D15/322 , B01D15/40 , B01D53/025 , B01J20/22 , B01J20/28004 , B01J20/28016 , B01J20/28042 , B01J20/28057 , B01J20/28059 , B01J20/28061 , B01J20/28064 , B01J20/28066 , B01J20/28069 , B01J20/28071 , B01J20/28073 , B01J20/28076 , B01J20/28078 , B01J20/28083 , B01J20/28085 , B01J20/288 , B01J20/289 , B01J20/3085 , B01J20/3204 , B01J20/3206 , B01J20/3208 , B01J20/3246 , B01J20/3285 , B01J20/3293 , C07F7/1804 , B01J20/282 , B01J20/284 , B01J20/285 , B01J20/32 , B01J20/3234 , B01J2220/54 , B01J2220/80
摘要： The present disclosure relates to a method of separating a compound of interest, particularly unsaturated compound(s) of interest, from a mixture. The compound is separated using a column having a chromatographic stationary phase material for various different modes of chromatography containing a first substituent and a second substituent. The first substituent minimizes compound retention variation over time under chromatographic conditions. The second substituent chromatographically and selectively retains the compound by incorporating one or more aromatic, polyaromatic, heterocyclic aromatic, or polyheterocyclic aromatic hydrocarbon groups, each group being optionally substituted with an aliphatic group. In some examples, the present disclosure can include a chromatographic system having a chromatographic column having a stationary phase with a chromatographic substrate containing silica, metal oxide, an inorganic-organic hybrid material, a group of block copolymers, or a combination thereof.
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公开(公告)号：US12078621B2
公开(公告)日：2024-09-03
申请号：US17699775
申请日：2022-03-21
发明人： Fabrice Gritti
CPC分类号： G01N30/461 , G01N30/82 , G01N2030/027 , G01N2030/3007 , G01N2030/326 , G01N2030/328
摘要： The disclosure relates to a gradient twin column recycling chromatography method that is used to separate a mixture containing closely eluting compounds. In one embodiment, a sample includes a primary organic compound and one or more impurities that closely elute with the primary organic compound. A gradient mobile phase is initially used to remove unwanted early eluting and late eluting impurities from the sample. After the gradient removal of some of the impurities is complete, the remaining mixture of the primary organic compound and the closely eluting impurities are separated using recycle chromatography methodology with an isocratic mobile phase.
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公开(公告)号：US12007370B2
公开(公告)日：2024-06-11
申请号：US17347807
申请日：2021-06-15
CPC分类号： G01N30/30 , G01N2030/025 , G01N2030/027 , G01N2030/3015 , G01N2030/3046
摘要： Exemplary embodiments may compensate for expected frictional heating or Joule-Thomson cooling in chromatography columns. Frictional heating or Joule Thomson cooling are the same thing for a fluid decompressing along a porous material. Either heat is absorbed from or released to the external environment. The exemplary embodiments may cool the mobile phase to a sub-ambient temperature before the mobile phase passes through a chromatography column to compensate for the frictional heating or heat the mobile phase to a super-ambient temperature to compensate for Joule-Thomson cooling. The amount of temperature increase expected from the frictional heating or the amount of temperature decrease expected from the Joule-Thomson cooling may be calculated or estimated. Based on the amount of temperature increase or decrease expected, the set point for the heater/cooler may be determined and applied to the mobile phase. The analyte may be injected solely into a central portion of the chromatography column to further compensate for thermal gradients.
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公开(公告)号：US12000806B2
公开(公告)日：2024-06-04
申请号：US17733218
申请日：2022-04-29
CPC分类号： G01N30/20 , B01D15/08 , G01N30/463 , G01N2030/027 , G01N30/34
摘要： Described are an interface module for two-dimensional chromatography and a method of performing a chromatographic separation that may use the interface module. The interface module includes a valve module, a collection needle, a modifier module and a sample storage element. The valve module has a first port configured to receive an eluent from a first chromatography system, a second port configured to provide a fraction obtained from the eluent, a third port and a fourth port. The collection needle and the modifier module are in fluidic communication with the valve module at the third and fourth ports, respectively. The modifier module includes a source of a modifier solvent. The sample storage element is in fluidic communication with the valve module and is configured to receive a volume of the fraction for injection as a sample into a second chromatography system.
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公开(公告)号：US11994500B2
公开(公告)日：2024-05-28
申请号：US17084998
申请日：2020-10-30
发明人： Mark J. Basile , Jeffrey Musacchio , Michael O. Fogwill , Sebastien Besner , Joseph D. Michienzi , Wade P. Leveille
CPC分类号： G01N30/74 , G01N30/30 , G01N2030/027 , G01N2030/3038 , G01N2030/746
摘要： Techniques and apparatus for thermally controlled interfaces for separation devices and optical flow cell devices with minimized post-column volumes are described. In one embodiment, for example, a column-optical cell assembly may include an insulating device, a chromatography column arranged within the insulating device, and an optical flow cell in fluid communication with the chromatography column, the chromatography column arranged within a minimum distance of the optical flow cell.
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公开(公告)号：US11986751B2
公开(公告)日：2024-05-21
申请号：US17241480
申请日：2021-04-27
发明人： Jonathan L. Belanger , Wade P. Leveille, Sr. , Michael O. Fogwill , Joseph D. Michienzi , Jeffrey Musacchio
CPC分类号： B01D15/22 , B01D15/10 , F16J15/34 , G01N30/60 , G01N30/6004 , G01N30/6026
摘要： Described are apparatuses, devices, and systems implementing seals for separation devices. An apparatus for a separation device may include an end nut shaped to be positioned on one of an inlet side and an outlet side of the separation device. The end nut may be further shaped to mate with a connecting fitting. The apparatus may further include a face seal having a device side and a fitting side. The face seal may be positioned, at least in part, in the end nut on the device side of the face seal. The face seal may be positioned to interface with the connecting fitting on the fitting side of the face seal upon the end nut being mated with the connecting fitting. The apparatus may also include a face seal bore of the face seal being axially alignable with at least one of an inlet and an outlet of the separation device.
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