公开(公告)号：US20250060342A1

公开(公告)日：2025-02-20

申请号：US18938818

申请日：2024-11-06

Applicant: Waters Technologies Corporation

Inventor： Michael O. Fogwill ,  Fabrice Gritti

IPC: G01N30/30 ,  G01N30/20 ,  G01N30/46 ,  G01N30/72

Abstract: Liquid chromatography systems described herein are smaller in size and with a reduced extra-column volume due to direct connection of a liquid chromatography column and/or detector to an injector valve and due to eliminating the need for a column oven by relying on heating of the liquid chromatography column and detector by a preheater. The size of the liquid chromatography systems are enough that the liquid chromatography systems may be deployed adjacent to automated sample preparation robotics, adjacent to a process stream, or adjacent to the inlet of a mass spectrometer. In addition, the extra-column volume of the liquid chromatography systems may be reduced by eliminating many of the connection tubes and by situating components of the liquid chromatography system in closer proximity.

公开(公告)号：US20250020619A1

公开(公告)日：2025-01-16

申请号：US18767095

申请日：2024-07-09

Applicant: Waters Technologies Corporation

Inventor： Lingyun Chen ,  Jianmin Liu

IPC: G01N30/54 ,  G01N30/02 ,  G01N30/30 ,  G01N33/02

Abstract: The present technology provides a method of eluting an analyte of interest from an immunoaffinity column. The methods of the present technology provide a simplified method for disassociating a bound analyte from the immunoaffinity column and allows for direct downstream processing of the eluate.

公开(公告)号：US20250012766A1

公开(公告)日：2025-01-09

申请号：US18893554

申请日：2024-09-23

Applicant: Waters Technologies Corporation

Inventor： Kerri M. Smith ,  Paul Rainville

IPC: G01N30/72 ,  C23C14/04 ,  C23C14/12 ,  G01N30/02

Abstract: The present disclosure discusses a method of separating a sample (e.g., small organic acid metabolite) including coating a flow path of a chromatographic system. The coating along the flow path is vapor deposited and prevents or severely decreases metal interactions between the metallic chromatographic system and the sample.

公开(公告)号：US12180581B2

公开(公告)日：2024-12-31

申请号：US17166460

申请日：2021-02-03

Applicant: Waters Technologies Corporation

Inventor： Stephen J. Shiner ,  Cheryl Boissel ,  Matthew A. Lauber ,  Kevin Wyndham

IPC: C23C16/02 ,  C09D143/04 ,  C23C14/04 ,  C23C14/12 ,  C23C14/16 ,  C23C14/58 ,  G01N30/56 ,  G01N30/60

Abstract: A chromatographic device including a coated metallic frit is disclosed. The coating is provided over the fluid exposed surfaces of the frit, thereby covering metallic surfaces to prevent interaction with an analyte in the flowstream. This technology relates to the use of vapor deposition coated frit(s) in a liquid flow path for improved chromatography. More specifically, this technology relates to liquid chromatographic devices for separating analytes in a sample having coated frit(s) within an uncoated metallic fluidic flow path (i.e., the column tube or channel is formed of stainless steel, titanium (pure or alloyed), or some mixture of stainless steel and titanium) and does not include the coating applied to the frit.

公开(公告)号：US20240390877A1

公开(公告)日：2024-11-28

申请号：US18796772

申请日：2024-08-07

Applicant: Waters Technologies Corporation

Inventor： Daniel P. Walsh ,  Kevin Daniel Wyndham ,  Jacob Nathan Cairchild ,  Nicole L. Lawrence

IPC: B01J20/283 ,  B01D15/20 ,  B01J20/22 ,  B01J20/28 ,  B01J20/285 ,  B01J20/286 ,  B01J20/32

Abstract: The present invention provides novel chromatographic materials, e.g., for chromatographic separations, processes for their preparation and separations devices containing the chromatographic materials. The chromatographic materials of the invention have controlled porosity and comprise a chromatographic core material and one or more layers of chromatographic surface materials which each independently provide an average pore diameter, an average pore volume, or a specific surface area such that the combined layers form a chromatographic material having a predetermined or desired pattern of porosity from the core material to the outermost surface. The materials are useful for HPLC separations, normal-phase separations, reversed-phase separations, chiral separations, HILIC separations, SFC separations, affinity separations, perfusive separations, partially perfusive separations, and SEC separations.

公开(公告)号：US12138617B2

公开(公告)日：2024-11-12

申请号：US17477347

申请日：2021-09-16

Applicant: Waters Technologies Corporation

Inventor： Nicole L. Lawrence ,  Yeliz Tunc Sarisozen ,  Darryl W. Brousmiche ,  Matthew A. Lauber

IPC: B01J20/289 ,  B01J20/10 ,  B01J20/26 ,  B01J20/28

Abstract: The present disclosure is directed to stationary phase materials for performing size exclusion chromatography. Embodiments of the present disclosure feature hydroxy-terminated polyethylene glycol surface modified silica particle stationary phase materials, which are optionally also methoxy-terminated polyethylene glycol surface modified.

公开(公告)号：US20240367123A1

公开(公告)日：2024-11-07

申请号：US18772812

申请日：2024-07-15

Applicant: Waters Technologies Corporation

Inventor： Neal Almeida

IPC: B01F29/15 ,  B01D15/18 ,  B01F23/43 ,  B01F23/70 ,  B01F23/80 ,  B01F101/00

Abstract: A solvent mixing system includes a mixing tee, a centrifugal mixing path, and a low frequency blending mixer. The mixing tee has at least two solvent input ports and a solvent output port in fluid communication with one another. The centrifugal mixing path has a mixing path inlet in fluid communication with the solvent output port of the mixing tee. The centrifugal mixing path includes at least one coiled segment between the mixing path inlet and a mixing path outlet. The low frequency blending mixer is in fluid communication with the outlet of the centrifugal mixing path.

公开(公告)号：US20240361318A1

公开(公告)日：2024-10-31

申请号：US18647863

申请日：2024-04-26

Applicant: Waters Technologies Corporation

Inventor： Kevin Wyndham ,  Beatrice Muriithi ,  Martin Gilar ,  Yeliz Tunc Sarisozen

IPC: G01N33/569 ,  G01N33/53 ,  G01N33/543 ,  G01N33/74

CPC classification number: G01N33/56983 ,  G01N33/5308 ,  G01N33/54313 ,  G01N33/54386 ,  G01N33/74 ,  G01N2333/015 ,  G01N2333/62 ,  G01N2469/10

Abstract: The present technology is directed to methods of preparing affinity chromatographic columns using streptavidin-conjugated particles and biotinylated affinity agents (i.e., biotinylated oligonucleotides, biotinylated antibodies or biotinylated antigen-binding fragments thereof). The methods of the present technology can be utilized to perform affinity capture assays in a high-throughput, efficient manner. Further, the affinity chromatographic columns disclosed herein are customizable due to the ability to utilize any biotinylated affinity agent. The methods herein utilize an on-line loading (i.e., loading the column with biotinylated affinity agent via a liquid chromatography system).

公开(公告)号：US12121829B2

公开(公告)日：2024-10-22

申请号：US16800928

申请日：2020-02-25

Applicant: Waters Technologies Corporation

Inventor： Mathew H. DeLano ,  Matthew A. Lauber ,  Michael Donegan ,  Robert A. Jencks ,  Stephen J. Shiner

IPC: B01D15/22 ,  C08J7/06 ,  G01N30/02 ,  G01N30/32 ,  G01N30/60

CPC classification number: B01D15/22 ,  C08J7/065 ,  G01N30/32 ,  G01N30/6026 ,  G01N30/603 ,  G01N30/6052 ,  G01N2030/027 ,  G01N2030/324

Abstract: The present disclosure relates to a filter. The filter includes a porous element, a compression element and a housing. At least a portion of the porous element is coated with an alkylsilyl coating. The compression element is configured to receive the porous element thereby forming an assembly. The housing has an opening formed therein. The opening is configured to receive the assembly. The assembly is retained within the opening when the assembly is received therein.

公开(公告)号：US20240335830A1

公开(公告)日：2024-10-10

申请号：US18749930

申请日：2024-06-21

Applicant: Waters Technologies Corporation

Inventor： Matthew A. Lauber ,  Mathew H. DeLano ,  Beatrice Muriithi ,  Anna Boardman

IPC: B01L3/00 ,  B01D15/38 ,  B01D15/42

CPC classification number: B01L3/5023 ,  B01D15/3809 ,  B01D15/424 ,  B01L2200/0631 ,  B01L2200/0668 ,  B01L2300/0681 ,  B01L2300/069 ,  B01L2400/0409 ,  B01L2400/0433

Abstract: A sample preparation device is provided for enriching a component of a sample. The sample preparation device includes a surface in fluid communication with the sample, an alkylsilyl coating disposed on the surface, and an affinity ligand or an enzyme covalently bonded to the alkylsilyl coating.