公开(公告)号：US20170184593A1

公开(公告)日：2017-06-29

申请号：US15300236

申请日：2015-12-18

申请人： WENZHOU MEDICAL UNIVERSITY

发明人： Xiaokun Li ,  Guang Liang ,  Xiaojie Wang ,  Shijun Li ,  Peisheng Hu

IPC分类号： G01N33/577

CPC分类号： G01N33/577 ,  G01N33/6872 ,  G01N2333/50 ,  G01N2800/347

摘要： This invention publicizes a chronic renal disease diagnostic kit, comprised of FGF23 monoclonal antibody, biotin labeling reagent Sulfo-NHS-LC-Biotin, PBS buffer solution (pH7.0, 0.1M), casein saline solution, streptomycin avidin and substrate TMB. The labeling condition is the weight ratio of FGF23 antibody and Sulfo-NHS-LC-Biotin, which is 1:5-1:12. The dilution ratio of streptomycin avidin is 1:3000. The lowest testing threshold limit value of FGF23 kit is 10 pg/ml, whose sensitivity is 10 times higher than that of common monoclonal antibody, which is very significant for early diagnose of chronic renal disease.