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公开(公告)号：US20210340532A1
公开(公告)日：2021-11-04
申请号：US16469677
申请日：2017-08-16
发明人： John Van Der Oost , Richard Van Kranenburg , Elleke Fenna Bosma , Ioannis Mougiakos , Prarthana Mohanraju
IPC分类号： C12N15/113 , C12N9/22 , C12N15/90 , C12N15/10
摘要： Thermostable Cas9 nucleases. The present invention relates to the field of genetic engineering and more particularly to nucleic acid editing and genome modification. The present invention provides an isolated Cas protein or polypeptide fragment thereof having an amino acid sequence of SEQ ID NO: 1 or a sequence of at least 77% identity therewith. The Cas protein or polypeptide is capable of binding, cleaving, marking or modifying a double stranded target polynucleotide at a temperature in the range 20° C. and 100° C. inclusive. The invention further provides isolated nucleic acid molecules encoding said Cas9 nucleases, expression vectors and host cells. The invention also provides PAM sequences recognized by the Cas protein or polypeptide, The Cas9 nucleases disclosed herein provide novel tools for genetic engineering in general, in particular at elevated temperatures and are of particular value in the genetic manipulation of thermophilic organisms; particularly microorganisms.
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公开(公告)号：US20220213455A1
公开(公告)日：2022-07-07
申请号：US17575952
申请日：2022-01-14
发明人： John Van Der Oost , Richard Van Kranenburg , Elleke Fenna Bosma , Ioannis Mougiakos , Prarthana Mohanraju
IPC分类号： C12N9/22 , C12N15/11 , C12N15/90 , C12N15/10 , C12N15/113
摘要： Thermostable Cas9 nucleases. The present invention relates to the field of genetic engineering and more particularly to nucleic acid editing and genome modification. The present invention provides an isolated Cas protein or polypeptide fragment thereof having an amino acid sequence of SEQ ID NO: 1 or a sequence of at least 77% identity therewith. The Cas protein or polypeptide is capable of binding, cleaving, marking or modifying a double stranded target polynucleotide at a temperature in the range 20° C. and 100° C. inclusive. The invention further provides isolated nucleic acid molecules encoding said Cas9 nucleases, expression vectors and host cells. The invention also provides PAM sequences recognized by the Cas protein or polypeptide, The Cas9 nucleases disclosed herein provide novel tools for genetic engineering in general, in particular at elevated temperatures and are of particular value in the genetic manipulation of thermophilic organisms; particularly microorganisms.
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公开(公告)号：US11326162B2
公开(公告)日：2022-05-10
申请号：US16469677
申请日：2017-08-16
发明人： John Van Der Oost , Richard Van Kranenburg , Elleke Fenna Bosma , Ioannis Mougiakos , Prarthana Mohanraju
IPC分类号： C12N15/113 , C12N9/22 , C12N15/90 , C12N15/10
摘要： Thermostable Cas9 nucleases. The present invention relates to the field of genetic engineering and more particularly to nucleic acid editing and genome modification. The present invention provides an isolated Cas protein or polypeptide fragment thereof having an amino acid sequence of SEQ ID NO: 1 or a sequence of at least 77% identity therewith. The Cas protein or polypeptide is capable of binding, cleaving, marking or modifying a double stranded target polynucleotide at a temperature in the range 20° C. and 100° C. inclusive. The invention further provides isolated nucleic acid molecules encoding the Cas9 nucleases, expression vectors and host cells. The invention also provides PAM sequences recognized by the Cas protein or polypeptide, The Cas9 nucleases disclosed herein provide novel tools for genetic engineering in general, in particular at elevated temperatures and are of particular value in the genetic manipulation of thermophilic organisms; particularly microorganisms.
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公开(公告)号：US11242513B2
公开(公告)日：2022-02-08
申请号：US16469674
申请日：2016-12-14
IPC分类号： C12N9/22 , C12N15/90 , C12N15/11 , C12N15/113 , C12N15/63
摘要： The present invention relates to the field of genetic engineering and more particularly to nucleic acid editing and genome modification. The present invention provides an isolated Cas protein or polypeptide fragment thereof having an amino acid sequence of SEQ ID NO: 1 or a sequence of at least 77% identity therewith. The Cas protein or polypeptide is capable of binding, cleaving, marking or modifying a double stranded target polynucleotide at a temperature in the range 30° C. and 100° C. inclusive. The invention further provides isolated nucleic acid molecules encoding the Cas9 nucleases, expression vectors and host cells. The invention also provides PAM sequences recognized by the Cas protein or polypeptide, The Cas9 nucleases disclosed herein provide novel tools for genetic engineering at elevated temperatures and are of particular value in the genetic manipulation of thermophilic organisms; particularly microorganisms.
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公开(公告)号：US20190322993A1
公开(公告)日：2019-10-24
申请号：US16469683
申请日：2017-08-16
发明人： John Van Der Oost , Richard Van Kranenburg , Elleke Fenna Bosma , Ioannis Mougiakos , Prarthana Mohanraju
摘要： Thermostable Cas9 nucleases. The present invention relates to the field of genetic engineering and more particularly to nucleic acid editing and genome modification. The present invention provides an isolated Cas protein or polypeptide fragment thereof having an amino acid sequence of SEQ ID NO: 1 or a sequence of at least 77% identity therewith. The Cas protein or polypeptide is capable of binding, cleaving, marking or modifying a double stranded target polynucleotide at a temperature in the range 20° C. and 100° C. inclusive. The invention further provides isolated nucleic acid molecules encoding said Cas9 nucleases, expression vectors and host cells. The invention also provides PAM sequences recognized by the Cas protein or polypeptide, The Cas9 nucleases disclosed herein provide novel tools for genetic engineering in general, in particular at elevated temperatures.
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