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公开(公告)号：US20230203448A1
公开(公告)日：2023-06-29
申请号：US18009231
申请日：2021-06-07
申请人： Erasmus University Medical Center Rotterdam , Academisch Ziekenhuis Leiden h.o.d.n. LUMC , Wageningen Universiteit
发明人： Alessandro IULIANO , Wilhelmus Wenceslaus Matthias PIJNAPPEL , Antje Tjitske VAN DER PLOEG , Vittorio SAGGIOMO , Jessica Christine DE GREEF , Erik VAN DER WAL , Silvere Maria VAN DER MAAREL
CPC分类号： C12N5/0658 , C12M21/08 , C12M23/16 , C12M25/14 , C12N2501/115 , C12N2501/727 , C12N2513/00 , B33Y10/00
摘要： A method of manufacturing a microstructure comprises printing a positive mold structure, filling the positive mold structure with a second material to form an elastically deformable negative mold structure, filling the negative mold structure with a third material to form the microstructure, and releasing the microstructure from the negative mold structure. Advantageously, the negative mold structure can be stretched to facilitate the release of the microstructure. For example, the microstructure comprises a chamber with capped micropillars for the generation and/or analysis of muscle tissue.
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公开(公告)号：US20220408782A1
公开(公告)日：2022-12-29
申请号：US17780244
申请日：2020-11-27
摘要： A cell (100) for making an anisotropic-structured product from a protein starting material, the cell (100) comprising an inner member (110) and an outer member (120) arranged concentrically along a longitudinal axis (180) to define together a chamber (150) for holding the starting material, wherein the inner member (110) and the outer member (120) are rotatable relative each other about the longitudinal axis (180) for providing the shear force to the starting material and wherein both the inner member (110) and the outer member (120) further define for the cell an inner longitudinal surface (114) and an outer longitudinal surface (124) facing away the chamber (150) such that a normal of each longitudinal surface is transverse to the longitudinal axis (180) characterized in that both members are designed to be exposed to the ambient for allowing a heat exchange between both the inner longitudinal surface (114) and the outer longitudinal surface (124) with the ambient to heat the chamber (150).
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公开(公告)号：US20220213455A1
公开(公告)日：2022-07-07
申请号：US17575952
申请日：2022-01-14
发明人： John Van Der Oost , Richard Van Kranenburg , Elleke Fenna Bosma , Ioannis Mougiakos , Prarthana Mohanraju
IPC分类号： C12N9/22 , C12N15/11 , C12N15/90 , C12N15/10 , C12N15/113
摘要： Thermostable Cas9 nucleases. The present invention relates to the field of genetic engineering and more particularly to nucleic acid editing and genome modification. The present invention provides an isolated Cas protein or polypeptide fragment thereof having an amino acid sequence of SEQ ID NO: 1 or a sequence of at least 77% identity therewith. The Cas protein or polypeptide is capable of binding, cleaving, marking or modifying a double stranded target polynucleotide at a temperature in the range 20° C. and 100° C. inclusive. The invention further provides isolated nucleic acid molecules encoding said Cas9 nucleases, expression vectors and host cells. The invention also provides PAM sequences recognized by the Cas protein or polypeptide, The Cas9 nucleases disclosed herein provide novel tools for genetic engineering in general, in particular at elevated temperatures and are of particular value in the genetic manipulation of thermophilic organisms; particularly microorganisms.
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公开(公告)号：US11326162B2
公开(公告)日：2022-05-10
申请号：US16469677
申请日：2017-08-16
发明人： John Van Der Oost , Richard Van Kranenburg , Elleke Fenna Bosma , Ioannis Mougiakos , Prarthana Mohanraju
IPC分类号： C12N15/113 , C12N9/22 , C12N15/90 , C12N15/10
摘要： Thermostable Cas9 nucleases. The present invention relates to the field of genetic engineering and more particularly to nucleic acid editing and genome modification. The present invention provides an isolated Cas protein or polypeptide fragment thereof having an amino acid sequence of SEQ ID NO: 1 or a sequence of at least 77% identity therewith. The Cas protein or polypeptide is capable of binding, cleaving, marking or modifying a double stranded target polynucleotide at a temperature in the range 20° C. and 100° C. inclusive. The invention further provides isolated nucleic acid molecules encoding the Cas9 nucleases, expression vectors and host cells. The invention also provides PAM sequences recognized by the Cas protein or polypeptide, The Cas9 nucleases disclosed herein provide novel tools for genetic engineering in general, in particular at elevated temperatures and are of particular value in the genetic manipulation of thermophilic organisms; particularly microorganisms.
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公开(公告)号：US11242513B2
公开(公告)日：2022-02-08
申请号：US16469674
申请日：2016-12-14
IPC分类号： C12N9/22 , C12N15/90 , C12N15/11 , C12N15/113 , C12N15/63
摘要： The present invention relates to the field of genetic engineering and more particularly to nucleic acid editing and genome modification. The present invention provides an isolated Cas protein or polypeptide fragment thereof having an amino acid sequence of SEQ ID NO: 1 or a sequence of at least 77% identity therewith. The Cas protein or polypeptide is capable of binding, cleaving, marking or modifying a double stranded target polynucleotide at a temperature in the range 30° C. and 100° C. inclusive. The invention further provides isolated nucleic acid molecules encoding the Cas9 nucleases, expression vectors and host cells. The invention also provides PAM sequences recognized by the Cas protein or polypeptide, The Cas9 nucleases disclosed herein provide novel tools for genetic engineering at elevated temperatures and are of particular value in the genetic manipulation of thermophilic organisms; particularly microorganisms.
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公开(公告)号：US11168335B2
公开(公告)日：2021-11-09
申请号：US16245767
申请日：2019-01-11
IPC分类号： C12N15/82 , A01H5/06 , C07K14/415 , A01H1/02 , C07K16/16
摘要： A R8 phytophthora resistance gene from potato is described that produces a protein capable of inferring oomycete resistance, such as resistance to Phytophthora infestans when expressed in a plant. Also described is a method for providing at least partial resistance or increasing resistance in a plant against an oomycete infection comprising providing a plant or a part thereof with a nucleotide sequence of this gene or a functional fragment thereof, for example wherein said plant is a plant from the Solanaceae family, more preferably Solanum tuberosum.
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公开(公告)号：US20210188923A1
公开(公告)日：2021-06-24
申请号：US17193562
申请日：2021-03-05
IPC分类号： C07K14/195 , C12N1/20 , A61P3/08 , A61P3/06
摘要： A method for cost-effectively and efficiently culturing Akkermansia muciniphila is described. High biomass yields can be obtained on chemically defined media. This allows for large scale production of A. muciniphila suitable for use in humans, such as for pharmaceutical or food applications. The A. muciniphila can be produced free of animal-derived products, thereby allowing a broad-range of applications.
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公开(公告)号：US20210137994A1
公开(公告)日：2021-05-13
申请号：US16976055
申请日：2019-03-01
IPC分类号： A61K35/37 , A61K9/00 , A61K45/06 , A61K35/74 , A61P37/06 , A61P3/10 , A61P5/14 , A61P5/38 , A61P17/06 , A61P17/00 , A61P19/02 , A61P1/00 , A61P11/06
摘要： The use of fecal matter in the treatment of a subject having autoimmune disease, wherein the fecal matter is autologous to the subject, and preferably administered to the small intestine, preferably the duodenum, of the subject. The fecal matter can be one or more constituents of autologous feces, preferably chosen from the group consisting of bacteria, viruses, bacteriophages, fungi, metabolites, microRNAs, proteins, antibodies, and/or antigens.
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公开(公告)号：US10736924B2
公开(公告)日：2020-08-11
申请号：US15759381
申请日：2016-09-09
发明人： Patrice Cani , Amandine Everard , Hubert Plovier , Céline Druart , Willem De Vos , Clara Belzer
IPC分类号： A61K35/741 , A61K9/00 , A23L33/105 , A61Q19/06 , A61K8/99 , A23L33/135 , A61P3/04
摘要： The present invention relates to pasteurized Akkermansia muciniphila or fragments thereof for treating a metabolic disorder in a subject in need thereof. The present invention also relates to a composition, a pharmaceutical composition and a medicament comprising pasteurized Akkermansia muciniphila or fragments thereof for treating a metabolic disorder. The present invention also relates to the use of pasteurized Akkermansia muciniphila or fragments thereof for promoting weight loss in a subject in need thereof.
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公开(公告)号：US20200010817A1
公开(公告)日：2020-01-09
申请号：US16400021
申请日：2019-04-30
发明人： John VAN DER OOST
摘要： The invention relates to the field of genetic engineering tools, methods and techniques for gene or genome editing. Specifically, the invention concerns isolated polypeptides having nuclease activity, host cells and expression vectors comprising nucleic acids encoding said polypeptides as well as methods of cleaving and editing target nucleic acids in a sequence-specific manner. The polypeptides, nucleic acids, expression vectors, host cells and methods of the present invention have application in many fields of biotechnology, including, for example, synthetic biology and gene therapy.
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