专利检索 ap:("Walter F Lima" OR "Timothy Vickers" OR "Stanley T Crooke") AND inv:"Walter F Lima" 第 1 页

1.

发明授权
Methods of determining potency of chemically-synthesized oligonucleotides 有权

公开(公告)号：US08093061B2

公开(公告)日：2012-01-10

申请号：US12445854

申请日：2007-10-18

申请人： Walter F Lima , Timothy Vickers , Stanley T Crooke

发明人： Walter F Lima , Timothy Vickers , Stanley T Crooke

IPC分类号： G01N33/53 , G01N33/00

CPC分类号： C12Q1/6876 , C12N15/111 , C12N2310/11 , C12N2310/14 , C12N2320/10 , Y10T436/143333

摘要： Provided herein are methods for determining potency of RNAi agents. Such methods include, but are not limited to, cell-based and cell-free assays that measure binding of an RNAi agent with Ago2 or that measure Ago2 activity in the presence of such RNAi agents. Also provided are assays that determine potency of RNAi agents by assessing their ability to compete with other RNAi agents, including control RNAi agents, for binding and/or activation of Ago2.